Uffelmann's Test PDF
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University of Northern Philippines - College of Medicine
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Summary
This document provides information on Uffelmann's test, a method used to detect the presence of lactic acid, for use in clinical biochemistry. The test procedure, principle, and color development are described.
Full Transcript
UNP - College of Medicine - 1F & 1E UFFELMAN'S TEST Biochemistry FOR LACTIC ACID DETECTION LACTIC ACID FORMATION Cellular FUNCTION OF Respiration LACTIC ACID Glucose Production Regulation of Inflammation Molecular...
UNP - College of Medicine - 1F & 1E UFFELMAN'S TEST Biochemistry FOR LACTIC ACID DETECTION LACTIC ACID FORMATION Cellular FUNCTION OF Respiration LACTIC ACID Glucose Production Regulation of Inflammation Molecular Signaling JULIUS UFFELMANN German physician REAGENT PREPARATION: 10mL of 1% Phenol + 1-2 drops of ferric chloride → IRON PHENOLATE PRINCIPLE UFFELMANN’S REAGENT + LACTIC ACID → IRON LACTATE + PHENOL COLOR DEVELOPMENT: LACTIC ACID: YIELDS TO A LIGHT YELLOW OR YELLOW-GREEN COLOR The test can be used to detect the LACTIC presence of lactic ACIDOSIS: acid, helping to diagnose conditions that lead to lactic acidosis, such as sepsis or certain metabolic diseases. LIMITATIONS It does not determine the concentration of lactic acid. It requires special precautionary measures. THANK YOU
