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Questions and Answers
What is the primary purpose of Uffelmann's test?
What is the primary purpose of Uffelmann's test?
- To monitor inflammation levels in tissues
- To measure the exact concentration of lactic acid
- To quantify glucose levels in the blood
- To detect the presence of lactic acid (correct)
What color indicates the presence of lactic acid in Uffelmann's test?
What color indicates the presence of lactic acid in Uffelmann's test?
- Dark green
- Blue
- Red
- Light yellow or yellow-green (correct)
What is a limitation of Uffelmann's test?
What is a limitation of Uffelmann's test?
- It does not provide information on lactic acid concentration (correct)
- It cannot be performed without heating the sample
- It is not sensitive to lactic acid levels in healthy individuals
- It requires a lengthy incubation period
Which of the following conditions could be diagnosed using Uffelmann's test?
Which of the following conditions could be diagnosed using Uffelmann's test?
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What is the main chemical reaction involved in Uffelmann's reagent testing?
What is the main chemical reaction involved in Uffelmann's reagent testing?
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Study Notes
Uffelmann's Test
- A test used for the detection of Lactic Acid
- Developed by Julius Uffelmann, a German physician
- Reagent Preparation:
- 10mL of 1% Phenol
- 1-2 drops of Ferric Chloride → Iron Phenolate
- Principle:
- Uffelmann's reagent reacts with Lactic Acid forming: Iron Lactate + Phenol
- Color Development:
- Lactic Acid yields a light yellow or yellow-green color
- Applications:
- Detects presence of Lactic Acid, which is valuable in diagnosing Lactic Acidosis
- Lactic Acidosis is caused by conditions like sepsis or metabolic diseases
- Limitations:
- Doesn't determine the concentration of Lactic Acid
- Requires special precautionary measures
Lactic Acid
- Formation: Occurs in the body through cellular respiration, particularly during anaerobic conditions
- Functions:
- Plays a role in cellular functions like glucose production
- Regulates inflammation
- Participates in molecular signaling
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