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Uffelmann's Test for Lactic Acid Detection

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Uffelmann's Test for Lactic Acid Detection

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Questions and Answers

What is the primary purpose of Uffelmann's test?

To monitor inflammation levels in tissues

To measure the exact concentration of lactic acid

To quantify glucose levels in the blood

To detect the presence of lactic acid (correct)

What color indicates the presence of lactic acid in Uffelmann's test?

Dark green

Blue

Red

Light yellow or yellow-green (correct)

What is a limitation of Uffelmann's test?

It does not provide information on lactic acid concentration (correct)

It cannot be performed without heating the sample

It is not sensitive to lactic acid levels in healthy individuals

It requires a lengthy incubation period

Which of the following conditions could be diagnosed using Uffelmann's test?

<p>Lactic acidosis</p> Signup and view all the answers

What is the main chemical reaction involved in Uffelmann's reagent testing?

<p>Iron phenolate reacting with lactic acid</p> Signup and view all the answers

Study Notes

Uffelmann's Test

A test used for the detection of Lactic Acid
Developed by Julius Uffelmann, a German physician
Reagent Preparation:
- 10mL of 1% Phenol
- 1-2 drops of Ferric Chloride → Iron Phenolate
Principle:
- Uffelmann's reagent reacts with Lactic Acid forming: Iron Lactate + Phenol
Color Development:
- Lactic Acid yields a light yellow or yellow-green color
Applications:
- Detects presence of Lactic Acid, which is valuable in diagnosing Lactic Acidosis
- Lactic Acidosis is caused by conditions like sepsis or metabolic diseases
Limitations:
- Doesn't determine the concentration of Lactic Acid
- Requires special precautionary measures

Lactic Acid

Formation: Occurs in the body through cellular respiration, particularly during anaerobic conditions
Functions:
- Plays a role in cellular functions like glucose production
- Regulates inflammation
- Participates in molecular signaling

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Description

This quiz covers Uffelmann's Test, a method for detecting lactic acid. Learn about the reagent preparation, the principle behind the test, and its applications in diagnosing lactic acidosis. Additionally, explore the limitations of the test and the role of lactic acid in cellular functions.