Cell Biology Notes PDF

RER EM - Cisternae With LM: Its place appears as localized basophilia due to the attached ribosomes. ▪ Site: abundant in protein synthesizing AL THERAPY cells ▪ With EM: they appear as: - Flattened interconnected membranous cisternae. - Their membranes are s...

RER EM - Cisternae With LM: Its place appears as localized basophilia due to the attached ribosomes. ▪ Site: abundant in protein synthesizing AL THERAPY cells ▪ With EM: they appear as: - Flattened interconnected membranous cisternae. - Their membranes are studied with ribosomes. FACULTY OF PHYSICAL THERAPY BAS1103  RER EM - Cisternae FACULTY OF PHYSICAL THERAPY BAS1103  SER EM - Tubules With LM: Not seen by LM ▪ With EM: they appear as: - Branching & anastomosing membranous tubules. - They have smooth membrane surface. - They have no ribosomes. ▪ FACULTY OF PHYSICAL THERAPY BAS1103  SER EM - Tubules FACULTY OF PHYSICAL THERAPY BAS1103  Golgi Apparatus It is a membranous organelle. With LM: not seen by LM. Size: variable; large Golgi leave an unstained area in the cytoplasm called -ve Golgi image AL THERAPY Stain: silver stain. With EM: It is formed of: Golgi saccules - Transfer vesicles- Secretory vesicles. FACULTY OF PHYSICAL THERAPY BAS1103  Golgi Apparatus - Silver stain FACULTY OF PHYSICAL THERAPY BAS1103  Golgi Apparatus EM - Saccules FACULTY OF PHYSICAL THERAPY BAS1103  1. Ribosomes Definition: Protein factory → formed of rRNA and protein They are non-membranous organelle. LM: They are too small to be visible. They impart their basophilia on the cytoplasm EM: They are electron dense particles. Free: single Attached: to RER. FACULTY OF PHYSICAL THERAPY BAS1103  Ribosomes -Toluidine blue Spotty basophilia FACULTY OF PHYSICAL THERAPY BAS1103  MICROTUBULES MICROTUBULES ▪ They are non-membranous organelles. ▪ With LM: can't be not seen. ▪ With EM: they appear as: - Straight slender tubes of variable length. - They are formed of protein tubulin 1  Centriole EM - Triplets ▪ It is non membranous organelle. ▪ Number: there are two centrioles per cell "one pair". ▪ With EM: - The two centrioles lie perpendicular to each other. - Each is a hallow cylinder. - Each made up of 27 microtubules - Microtubules are arranged in 9 bundles. - Each bundle is formed of 3 microtubules "Triplets" ▪. 11  Centriole EM - Triplets FACULTY OF PHYSICAL THERAPY 12 BAS1103  Cilia They are non-membranous organelles. ▪ With LM: they appear as hair like processes at the cell surface. Cilia ▪With EM: Each cilium has: Basal body: below the cell surface. Shaft or axoneme: that project from the cell surface. 1- Basal body: Identical to centriole (27 microtubules) 2- Shaft: ▪ It is formed of 20 microtubules arranged as [9 doublets + 2 central microtubules] ▪ ▪ movement over cell surface. FACULTY OF PHYSICAL THERAPY BAS1103  Cilia T.S. EM - Doublets FACULTY OF PHYSICAL THERAPY 14 BAS1103  EM of cilia (LS) Microtubules Shaft Cell membrane 1- Basal body: Basal body Identical to centriole (27 microtubules arranged as 9 triplets + 0) FACULTY OF PHYSICAL THERAPY BAS1103  Cilia L.S. EM FACULTY OF PHYSICAL THERAPY 16 BAS1103  Compare centriole and cilia FACULTY OF PHYSICAL THERAPY BAS1103  Fat FACULTY OF PHYSICAL THERAPY BAS1103  Fat - Sudan III FACULTY OF PHYSICAL THERAPY BAS1103  Glycogen - PAS FACULTY OF PHYSICAL THERAPY BAS1103  Compare mitochondria and glycogen FACULTY OF PHYSICAL THERAPY BAS1103 FACULTY OF PHYSICAL THERAPY BAS1103 FACULTY OF PHYSICAL THERAPY BAS1103 Which of the following would be used to visualize Golgi? a- silver b- toluidine blue. c- Sudan III d- Iron hematoxylin Which of the following would be used to visualize fat? a- Silver b- Trypan blue. c- Sudan III d- Iron Issue 1/hematoxylin 2018 1 Mention the staining character for each of the following: Ribosomes, lipids of fat cells, glycogen of muscles and liver. Which of the following would be used to visualize glycogen? a- PAS b- toluidine blue. c- Sudan III d- Iron hematoxylin 6 In sections stained with H&E, Golgi apparatus appears as: a- Basophilic structures b- Acidophilic structures c- Brown granules D-unstained area Cytoplasmic basophilia indicates presence of: a- Mitochondria b- Golgi c- rER d- sER FACULTY OF PHYSICAL THERAPY BAS1103