Biology Lab Test Bank Mid Exam PDF

Summary

This document is a set of biology lab questions covering topics such as microscopy, cell structure, and biological processes. The document contains multiple choice questions for a mid-exam.

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Experiment 1 To measure the total magnification of compound microscope (Ocular x20) we…: a) Add 20 times to objective lens magnification. b) Multiply objective lens magnification By 20 c) Add 200 to objective lens magnification. d) Multiply objective lens magnification. By 200 Which of the followi...

Experiment 1 To measure the total magnification of compound microscope (Ocular x20) we…: a) Add 20 times to objective lens magnification. b) Multiply objective lens magnification By 20 c) Add 200 to objective lens magnification. d) Multiply objective lens magnification. By 200 Which of the following regulate the light intensity? a) Light source b) Condensor c) Adobe knob d) Iris diaphragm Which part of the compound microscope concentrates and directs the light through the specimen: a) Course focus adjustment knob b) Two stage adjustment knobs (mechanical stage control or adobe knob) c) The condenser lenses d) The iris diaphragms The magnification of Simple and Compound microscopes respectively is: a) 200-300X, 1200X b) 1200X, 200-300X c) 10-50X, 100X d) 100X, 10-50X Magnification of the high-powered lens of a compound microscope is: a) 4X b) 10X c) 40X d) 100X Total magnification of the Low-powered lens is: a) 40X b) 100X c) 400X d) 1000X In amoeba, regulation of the amount of water in the cell and osmotic pressure by removing water is done by: a) Nucleus b) Pseudopodia c) Food vacuole d) Contractile vacuole The characteristic of the image that is produced by light microscope is true inverted: a) True b) False the type of slides that contain fresh, living biological sample is: a) prepared slide b) wet slide Which of the following is correct procedure when you finish using a microscope? a) The stage should be at the highest point b) Put the microscope in the cabinet in such a way that the microscopic arm faced out. c) Leave the electric wire untied d) Leave the microscope on the lab bench One of the following is incorrect when working with chemicals: a) Use a single pipette for all chemicals b) Immediately flush the area of your skin that was exposed to any chemical and notify your instructor c) Check the label on the chemical bottle before using it d) Wear safety goggles Which one of the following concepts is used to describe the ability to separate or distinguish between small adjacent images? A. contrast B. resolution C. magnification D. diameter or field of view Who is the first person to record data from a microscope? A. Chester Carlson B. Ami Argand C. Antoni Van Leeuwenhoek D. Galileo Galilee We put the cover on the slide in 45 degree to: Avoid the air bubbles We use ……………….. to remove excess water from the slide: A. Cotton paper B. Pipette C. Filter paper D. A cloth The part pointed at with the red arrow is called: a) Food Vacuole b) Nucleus c) Endoplasm d) Contractile Vacuole Experiment 2 Which of the following statements is correct? a) Prokaryotic cells are larger than eukaryotic cells b) Cytoskeleton is present in both eukaryotic and prokaryotic cells. c) Prokaryotic cells lack histone. d) eukaryotic cells have 70s ribosomes in their cytoplasm while prokaryotic cells have 80s ribosomes in their cytoplasm. The “A” in the picture points to a) Large vacuole b) Guard cell c) Chloroplast d) Stoma The stain used to stain cheek epithelium is Lugol’s Iodine stain: a) True b) False To prevent trapping of the air bubbles the coverslip should be lowered quickly to not give the air a chance to be trapped. a) True b) False Plasmodesmata is: a) Site of storage of pigments b) A channel connecting two cells c) Filled with cell sap and maintains pressure against cell wall d) Site of photosynthesis Which of the following is not found in animal cell a) Lysosomes b) Plasmodesmata c) Centriole d) Golgi complex One of the following is not found in eukaryotic cells a) Circular chromosome b) Nucleus c) Flagella d) Plastids The “C” in the figure is a a) Epidermal cell b) Chloroplast c) stoma d) Guard cell In this figure these cells are …… and this organism is called ………….. a) Eukaryotic – Bacillus b) Prokaryotic – Paramecium c) Prokaryotic – Bacillus d) Eukaryotic – Paramecium Chloroplast is important in: a) Protein Synthesis b) Photosynthesis c) Cell movement d) Digestion of waste materials Regarding the figure, the structure that is not found in plant cells is: a) 9 b) 6 c) 1 d) 13 All of the following are used in preparation of cheek epithelium EXCEPT: a) Lugol's iodine b) Dissecting needle c) Tooth pick d) Filter paper The cytoplasmic region where the chromosome ____ lies in the prokaryotic cells called a) Nuclease b) Nucleoid c) Nucleus d) Pili A student saw cell under the microscope with definite shape and double-membrane organelles. this cell is. _ __ _ a) Plant and bacterial cells b) Plant cell c) Bacterial cell d) Animal cell Which of the following is a compartment that often takes up much of the volume of a plant cell a) Golgi apparatus b) Lysosome c) Vacuole d) Mitochondrion All of the following is true about the cell wall EXCEPT: a) Maintain the cell shape b) Found in both plant and bacteria c) Protect the internal organelles of the cell d) It has the same composition in both plant and bacteria The function of Ribosomes is a) Maintenance of cell shape b) Lipid production c) Protein synthesis d) Energy production The function of lysosome is: a) Passage to and out of nucleus b) Break down macromolecules c) Secrete material from the cell d) Energy house Experiment 3 Starch is a chain composed of two portions, …………………….. is the straight portion that gives a …………………………. Color in lugol’s test, while ……………………….. Is the branch potion that gives a …………….. Color. a. Amylopectin, yellow/orange – Amylose, blue /black b. Amylopectin, blue /black – Amylose, yellow / orange c. Amylose, blue /black – Amylopectin, yellow / orange d. Amylose, yellow / orange – Amylopectin, blue / black The following juices and their corresponding number of drops were used to turn indophenol into colorless: Juice A (22 drops), Juice B (9 Drops) and Juices C (14 Drops). Arrange the juices in a descending order according to their concentration of vitamin C? A. B>C>A B. C>B>A C. A>B>C Which of the following is a reducing sugar? a. Fructose b. Starch c. trehalose d. Sucrose Biuret test reveals the presence of: a. Covalent bond b. Peptide Bond. c. H-bond d. Any ester bond In ascorbic acid test, the indicator (reagent) used was: a. Indophenol blue b. Benedict blue c. biuret blue d. Lugol's iodine The sample which used as the reference or negative control with all chemical tests is a. Biuret reagent b. Distilled water c. Copper sulfate d. Lugol's iodine The reaction of which a macromolecule breaks down to smaller subunits is called a. Hydrolysis b. Dehydration Benedict reagent consist of a. Copper (II) Sulfate and sodium hydroxide b. Copper (II) Sulfate and sodium citrate and sodium carbonate c. Sodium citrate and sodium carbonate d. Sodium citrate and copper (II) sulfate In benedicts test why did we test D.H2O? a. It was a negative control b. It was a positive control c. To see if it has any reducing sugars What is the color change pattern during the biuret test (initial to final) a. Blue to violet b. Violet to pink to blue c. Violet to blue d. Blue to pink or violet To form a polymer, monomers were linked together in a ……………. Reaction where water is …………….. a. Dehydration, released b. Hydrolysis, added c. Dehydration, added d. Hydrolysis, released in the figure above, violet color indicates the presence of a. Reducing sugar b. Protein c. Starch d. Non reducing sugar Solution (A) contains fructose and water and solution (B) contains trehalose and water, which test is used to differentiate solution (A) from solution (B) a. Benedict b. Biuret c. lugol's d. a and c All of the following are true about the test in the figure except? a. Needs heat b. Galactose give positive result c. The red color result from copper sulfate precipitate d. The reagent for this test contains copper sulfate, sodium carbonate, and sodium citrate the results in the figure are for a test that detect ………….. , and the reagent used in the test was? a. Reducing sugars, Ether b. Reducing sugars, Lugol's c. Any sugar, ether d. Sugars with free aldehyde groups, benedicts b. The product that an enzyme acts on when it catalyzes a chemical reaction c. None of them d. Two answers are correct The optimum temperature of catalase is: a. 37 b. 25 c. 10 If we move away from the optimum pH of the enzyme it might be: a. Denatured b. Deactivated c. Both We can return the function of the enzyme the was in the ice pocket by heating it up. a. True b. False Experiment 5 Which of the following statements is false regarding glycolysis? a. Glycolysis produces a net of 2 ATP, 2NADH, and 2 pyruvate b. Glycolysis takes place in the mitochondria of a cell c. Glycolysis can occur without the presence of Oxygen d. Glycolysis is the first step in aerobic respiration In fermentation, the ATP is produced by: a. Chemiosmosis b. Electron transport chain c. Glycolysis d. Krebs cycle In the lab, exhalation process release____which react with_______ to form____: a. O2/Barium hydroxide/ Barium carbonate, respectively. b.CO2/Calcium carbonate/ Calcium hydroxide, respectively c. CO2/Barium hydroxide/ Barium carbonate, respectively d. CO2/Barium carbonate/Barium hydroxide, respectively What is the function of NADH in cellular respiration? a. NADH carries the oxygen needed to break down the glucose molecules into the cell b. NADH carries excess oxygen out of the cell e. NADH carries excess carbon dioxide out of the cell d. NADH carries and transfers electrons and H ions The carbon source that used in dehydrogenase activity is: a. Yeast b. Sucrose c. Glucose d. Methylene blue One of the followings is NOT end products of alcohol fermentation: a. ATP b. ethanol c. CO2 d. NADH In aerobic respiration, most of ATP is produced by: a. Glycolysis b. Chemiosmosis c. Krebs cycle d. Electron transport chain Dehydrogenase enzyme activity was detected by using: a. Indophenol blue b. Barium hydroxide c. lodine d. Methylene blue In aerobic respiration of yeast, we used methylene blue as: a. Electron donor b. Proton donor c. Oxidizing agent d. Reducing agent The apparatus in the following picture is called: a. Respiratory chamber b. Thermometer c. Dehydrogenase activity set d. Anaerobic respiration set The part of the cell where glycolysis takes place is: a. Cytoplasm (cytosol) b. Nucleus c. Mitochondria d. Ribosome To detect the liberation of CO2 by respiration , the exhale bottle is half filled with : a. CaCO3 b. NaHCO3 c. Ba(OH)2 d. HCO3- The inhale bottle , which one of the following formed after inhalation ? a. Barium carbonate b. Nothing formed c. Barium Hydroxide d. Barium bicarbonate In experiment of dehydrogenase activity, the methylene blue is __ to from___ a. Oxidized, Leukomethylene, respectively b. Lose proton, Leukomethylene, respectively c. Reduced, Leukomethylene, respectively d. Lose 2 electrons, Leukomethylene, respectively Clear my choice In the picture below, tube (2) contains: a. Glucose + fresh yeast b. Sucrose + fresh yeast c. Ba(OH)2+ fresh yeast d. Glucose + dead yeast In dehydrogenase experiment, which of the following shows the highest rate of dehydrogenase activity? a. Glucose + living yeast + methylene blue at 50° b. Glucose + living yeast + methylene blue at 37° c. Glucose + living yeast+ methylene blue at 4° d. Glucose + living yeast methylene blue at Boiling Which of the following experiments indicats that water liberates by aerobic respiration ? a. Dehydrogenase test b. water droplets on cooled glass c. Exhalation / inhalation test d. respiration chamber test In figure 1 A dehydrogenase activity experiment done, tube1"means that: a. The yeast is Iive and methelyne blue turns to leukomethylene b. The yeast is dead and leukomethylene converts to methelyne blue c. The yeast is live and leukomethylene converts to methelyne blue d. The yeast is dead and leukomethylene remains coloriess Good luck everyone!

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