LAB_Study_Guide_1_f17.pdf

LAB Study Guide – Practical I f17 version this is a word document – you can add spaces before you print. 1. WEEK 1 Know Lab Syllabus Know Lab Safety contract and location of safety equipment Ex 25 – Handwashing How did we do experiment? What...

LAB Study Guide – Practical I f17 version this is a word document – you can add spaces before you print. 1. WEEK 1 Know Lab Syllabus Know Lab Safety contract and location of safety equipment Ex 25 – Handwashing How did we do experiment? What trends did we observe? C / C transient and resident microbes. Give examples. What are the conditions under which we incubated our plates /cultures? What are standard condition of incubation for bacterial cultures? Review questions on worksheet: 1,2,4,5 / 1,2 / 2,3,4 Ex 4 Microbes are Everywhere What are fomites? How did we do this experiment? Samples were streaked on type of plates: environmental: skin/mucous membranes: What trends did we observe? What was seen on cough plate and air plate? What are alpha, beta and gamma hemolysis? Name at least 4 modes of transmission. Review questions: t / f 1-4 2. Week 2 Know Ex 1 - Microscope Parts and use of the parts Know proper Microscope usage Microscope put away checklist Recognize and correctly name shapes and arrangements of bacteria. Review questions on worksheet: 1,3,4,5,6 / 2,3 / 1,2,3,4 How do you do aseptic technique? How does one flame sterilize a loop – be specific Describe a broth, slant, deep. What is the purpose of flaming the openings of the test tubes? What is a pure culture? Review worksheet questions: 2,4 / 1,2,3 / 3 3. Week 3 Ex 6 Smear: What should a good quality smear look like? Define and give examples of basic stains and acidic stains. What is the purpose of heat fixation? Unstained cells are: Ex 8 Gram Stain How do you do a gram stain? What stains are used? What results did we observe, and how are they interpreted? Review worksheet questions:1,2,5 / 1,2,3,4 / 1,2 Ex 7 Negative Stain How is the negative stain done? What stain is used? What is the charge of this stain? For what is the negative stain useful? What is observed? 4. Week 4 a. Ex 9 Acid Fast Stain b. How is this stain done?  c. What stains are used? d. When and why is steam used? e. What results did we observe, and how are they interpreted? f. Review Worksheet questions: 1,2,3,4 / 1,2,3,4 / 2,4 g. Ex 10 Endospore Stain – endospore – not spore h. How is this stain done? i. What stains are used? j. When and why is steam used? k. What results did we observe, and how are they interpreted? l. Review Worksheet questions: 2,3,4,5 / 1,3,4 / 1,2,3,4 5. Week 5 Ex 2 Fungi What kingdom are the fungi in? Are they eukaryotes or prokaryotes? Can they photosynthesize? Define dimorphic as it applies to fungi. What are hyphae? Mycelium? How can they reproduce? Rhizopus slide: recognize. Recognize sporangia and zygospores. Penicillium slide: recognize. Recognize conidia. Aspergillus slide: recognize. Recognize conidia. Candida slide: recognize. Recognize growth form. (contrast with above) Be able to recognize and name the four plates (living cultures) you observed. Know what diseases are caused by Rhizopus, Penicillium, Aspergillus, Candida. Review Worksheet questions: 1,4 / 1,4 a. Ex 3 (deleted word Phylum) Protozoa b. Be able to recognize and name the 7 slides we viewed. c. Name a disease for each of the 7 above. d. What kingdom are the Protozoa in? e. Can they grow in a multicellular form? f. Eukaryotic or prokaryotic cells? g. Trypanozoma cruzi h. Trypanosoma brucei  i. Trichomonas j. Leishmania k. Giardia l. Balantidium m. Plasmodium n. Toxoplasma o. Review Worksheet questions: 2,5 / 2 / 1 p. Exercise 34 – Fermentation Tests What products can be produced by fermentation? What media did we use? Phenol red turns what color when pH is below 6.8? What is fermentation? Remember Ch 5 in Lecture….What are catabolic reactions? What is the purpose of the Durham tubes? What is the purpose of the phenol red? What sugars were tested? What is homolactic fermentation? What is heterolactic fermentation? What will be seen in the broths set with non fermenters? How did we read the results? Review Worksheet: we only do the phenol red sugar broth test. Questions: 1, 4