All-ISEF-Forms (1).pdf

Checklist for Adult Sponsor (1) This completed form is required for ALL projects. To be completed by the Adult Sponsor in collaboration with the student researcher(s): Student’s Name(s): Project Title: 1....

Checklist for Adult Sponsor (1) This completed form is required for ALL projects. To be completed by the Adult Sponsor in collaboration with the student researcher(s): Student’s Name(s): Project Title: 1. o I have reviewed the ISEF Rules and Guidelines, including the science fair ethics statement. 2. o I have reviewed the student’s completed Student Checklist (1A) and Research Plan/Project Summary. 3. o I have worked with the student and we have discussed the possible risks involved in the project. 4. o The project involves one or more of the following and requires prior approval by an SRC, IRB, IACUC or IBC: o Humans Potentially Hazardous Biological Agents o Vertebrate Animals o Microorganisms o rDNA o Tissues 5. o Items to be completed for ALL PROJECTS o Adult Sponsor Checklist (1) o Research Plan/Project Summary o Student Checklist (1A) o Approval Form (1B) o Regulated Research Institutional/Industrial Setting Form (1C) (when applicable; after completed experiment) o Continuation/Research Progression Form (7) (when applicable) Additional forms required if the project includes the use of one or more of the following (check all that apply): o Humans, including student designed inventions/prototypes. (Requires prior approval by an Institutional Review Board (IRB); see full text of the rules.) o Human Participants Form (4) or appropriate Institutional IRB documentation o Sample of Informed Consent Form (when applicable and/or required by the IRB) o Qualified Scientist Form (2) (when applicable and/or required by the IRB) o Vertebrate Animals (Requires prior approval, see full text of the rules.) o Vertebrate Animal Form (5A) ‑ for projects conducted in a school/home/field research site (SRC prior approval required o Vertebrate Animal Form (5B) ‑ for projects conducted at a Regulated Research Institution. (Institutional Animal Care and Use Committee (IACUC) approval required prior experimentation.) o Qualified Scientist Form (2) (Required for all vertebrate animal projects at a regulated research site or when applicable) o Potentially Hazardous Biological Agents (Requires prior approval by SRC, IACUC or IBC, see full text of the rules.) o Potentially Hazardous Biological Agents Risk Assessment Form (6A) o Human and Vertebrate Animal Tissue Form (6B) ‑ to be completed in addition to Form 6A when project involves the use of fresh or frozen tissue, primary cell cultures, blood, blood products and body fluids. o Qualified Scientist Form (2) (when applicable) o The following are exempt from prior review but require a Risk Assessment Form 3: projects involving protists, archae and similar microorganisms, for projects using manure for composting, fuel production or other non-culturing experiments, projects using color change coliform water test kits, microbial fuel cells, and projects involving decomposing vertebrate organisms. o Hazardous Chemicals, Activities and Devices (No SRC prior approval required, see full text of the rules.) o Risk Assessment Form (3) o Qualified Scientist Form (2) (required for projects involving DEA-controlled substances or when applicable) o Other o Risk Assessment Form (3) o I attest to the information checked above and that I have read and agree to abide by the science fair ethics statement. Adult Sponsor’s Printed Name Signature Date of Review (mm/dd/yy) Phone Email International Rules: Guidelines for Science and Engineering Fairs 2024–2025, societyforscience.org/ISEF Page 31  Student Checklist (1A) This form is required for ALL projects. 1. a. Student/Team Leader: Grade: Email: Phone: b. Team Member: c. Team Member: ______________________________________ 2. Title of Project: ___________________________________________________________________________________________________________ 3. School: School Phone: (if multiple schools, list of the team leader or list all schools). School Address: 4. Adult Sponsor: Phone/Email: 5. Does this project need SRC/IRB/IACUC or other pre-approval? o Yes o No Tentative start date: ___________ 6. Is this a continuation/progression from a previous year? o Yes o No If Yes: a. Attach the previous year’s o Abstract and o Research Plan/Project Summary b. Explain how this project is new and different from previous years on o Continuation/Research Progression Form (7) 7. This year’s experimentation/data collection: Actual Start Date: (mm/dd/yy) End Date: (mm/dd/yy) 8. Where will you conduct your experimentation? (check all that apply) o Research Institution o School o Field o Home o Other: ________________________ 9. Source of Data: o Collected self/mentor o Other Describe/url: _____________________________________________  ist the name and address of all non-home and non-school work site(s), whether you worked there 10. L virtually or on-site: Name Address: Phone/email 11. Complete a Research Plan/Project Summary following the Research Plan/Project Summary instructions and attach to this form.  n abstract is required for all projects after experimentation. 12. A Page 32 International Rules: Guidelines for Science and Engineering Fairs 2024–2025, societyforscience.org/ISEF  Research Plan/Project Summary Instructions A complete Research Plan/Project Summary is required for ALL projects and must accompany Student Checklist (1A). All projects must have a Research Plan/Project Summary a. The Research Plan is to be written prior to experimentation following the instructions below to detail the rationale, research question(s), methodology, and risk assessment of the proposed research. b. If changes are made during the research, such changes can be added to the original research plan as an addendum, recognizing that some changes may require returning to the IRB or SRC for appropriate review and approvals. If no additional approvals are required, this addendum serves as a project summary to explain research that was conducted. c. If no changes are made from the original research plan, no project summary is required. Some studies, such as an engineering design or mathematics projects, will be less detailed in the initial project plan and will change through the course of research. If such changes occur, a project summary that explains what was done is required and can be appended to the original research plan. The Research Plan/Project Summary should include the following: a. RATIONALE: Include a brief synopsis of the background that supports your research problem and explain why this research is important and if applicable, explain any societal impact of your research. b. RESEARCH QUESTION(S), HYPOTHESIS(ES), ENGINEERING GOAL(S), EXPECTED OUTCOMES: How is this based on the rationale described above? c. Describe the following in detail: List of materials: Procedures: Detail all procedures and experimental design including methods for data collection, and when applicable, the source of data used. Describe only your project. Do not include work done by mentor or others. Risk and Safety: Identify any potential risks and safety precautions needed. Data Analysis: Describe the procedures you will use to analyze the data/results. d. BIBLIOGRAPHY: List major references (e.g. science journal articles, books, internet sites) from your literature review. If you plan to use vertebrate animals, one of these references must be an animal care reference. Items 1–4 below are subject-specific guidelines for additional items to be included in your research plan/project summary as applicable. 1. Human participants research: a. Participants: Describe age range, gender, racial/ethnic composition of participants. Identify vulnerable populations (minors, pregnant women, prisoners, mentally disabled or economically disadvantaged). b. Recruitment: Where will you find your participants? How will they be invited to participate? c. Methods: What will participants be asked to do? Will you use any surveys, questionnaires or tests? If yes and not your own, how did you obtain? Did it require permissions? If so, explain. What is the frequency and length of time involved for each subject? d. Risk Assessment: What are the risks or potential discomforts (physical, psychological, time involved, social, legal, etc.) to participants? How will you minimize risks? List any benefits to society or participants. e. Protection of Privacy: Will identifiable information (e.g., names, telephone numbers, birth dates, email addresses) be collected? Will data be confidential/anonymous? If anonymous, describe how the data will be collected. If not anonymous, what procedures are in place for safeguarding confidentiality? Where will data be stored? Who will have access to the data? What will you do with the data after the study? f. Informed Consent Process: Describe how you will inform participants about the purpose of the study, what they will be asked to do, that their participation is voluntary and they have the right to stop at any time. 2. Vertebrate animal research: a. Discuss potential ALTERNATIVES to vertebrate animal use and present justification for use of vertebrates. b. Explain potential impact or contribution of this research. c. Detail all procedures to be used, including methods used to minimize potential discomfort, distress, pain and injury to the animals and detailed chemical concentrations and drug dosages. d. Detail animal numbers, species, strain, sex, age, source, etc., include justification of the numbers planned. e. Describe housing and oversight of daily care. f. Discuss disposition of the animals at the end of the study. Potentially hazardous biological agents research: a. Give source of the organism and describe BSL assessment process and BSL determination. b. Detail safety precautions and discuss methods of disposal. 4. Hazardous chemicals, activities & devices: a. Describe Risk Assessment process, supervision, safety precautions and methods of disposal. b. Material Safety Data Sheets are not necessary to submit with paperwork. International Rules: Guidelines for Science and Engineering Fairs 2024–2025, societyforscience.org/ISEF Page 33  Approval Form (1B) A completed form is required for each student, including all team members. 1. To Be Completed by Student and Parent a. Student Acknowledgment: I understand the risks and possible dangers to me of the proposed research plan. I have read the ISEF Rules and Guidelines and will adhere to all International Rules when conducting this research. I have read and will abide by the science fair ethics statement. Student researchers are expected to maintain the highest standards of honesty and integrity. Scientific fraud and misconduct are not condoned at any level of research or competition. Such practices include but are not limited to plagiarism, forgery, use or presentation of other researcher’s work as one’s own, and fabrication of data. Fraudulent projects will fail to qualify for competition in affiliated fairs and ISEF. Student’s Printed Name Signature Date Acknowledged (mm/dd/yy) (Must be prior to experimentation.) b. Parent/Guardian Approval: I have read and understand the risks and possible dangers involved in the Research Plan/Project Summary. I consent to my child participating in this research. Parent/Guardian’s Printed Name Signature Date Acknowledged (mm/dd/yy) (Must be prior to experimentation.) 2. To be completed by the local or affiliated Fair SRC (Required for projects requiring prior SRC/IRB APPROVAL. Sign 2a or 2b as appropriate.) a. Required for projects that need prior SRC/IRB approval b.Required for research conducted at all Regulated BEFORE experimentation (humans, vertebrates or Research Institutions with no prior fair SRC/IRB potentially hazardous biological agents). OR approval. This project was conducted at a regulated research institution The SRC/IRB has carefully studied this project’s Research Plan/ (not home or high school, etc.), was reviewed and approved Project Summary and all the required forms are included. My by the proper institutional board before experimentation and signature indicates approval of the Research Plan/Project complies with the ISEF Rules. Attach (1C) and any required Summary before the student begins experimentation. institutional approvals (e.g. IACUC, IRB). SRC/IRB Chair’s Printed Name SRC Chair’s Printed Name Signature Date of Approval (mm/dd/yy) (Must be prior to experimentation.) Signature Date of Signature (mm/dd/yy) (May be after experimentation) 3. Final ISEF Affiliated Fair SRC Approval (Required for ALL Projects) SRC Approval After Experimentation and Before Competition at Regional/State/National Fair I certify that this project adheres to the approved Research Plan/Project Summary and complies with all ISEF Rules. Regional SRC Chair’s Printed Name Signature Date of Approval (mm/dd/yy) State/National SRC Chair’s Printed Name Signature Date of Approval (mm/dd/yy) (where applicable) Page 34 International Rules: Guidelines for Science and Engineering Fairs 2024–2025, societyforscience.org/ISEF  Revised–Regulated Research Institutional/Industrial Setting Form (1C) This form must be completed AFTER experimentation by the adult supervising the student research either virtually or on site, conducted in a regulated research institution, industrial setting or any work site other than home, school or field. Student’s Name(s) Title of Project To be completed by the Supervising Adult in the Setting (NOT the Student(s)) after experimentation: (Responses must be on the form as it is required to be displayed at student’s project booth; please do not print double-sided.) Research was supported at my work site: 1. Describe the student experience at your work site (check all that apply): Used Equipment o Yes o No Minimal interaction with our group o Yes o No Mentored by me or someone else from our group o Yes o No Worked as a sub-set of our ongoing research o Yes o No Had an independent project from our group o Yes o No 2. Please describe the independent and/or creative work done by the student in any phase of the project, but particularly in developing the hypotheses or engineering goals of the project 3. Detail the student’s role in conducting the research (e.g. data collection, specific procedures performed). Differentiate what the student observed and the student actually did. 4. Did the student(s) work on the project as part of a group? o Yes o No Were there other high school students present? If yes, please list the students names and describe how their work was related or different from the work of this projecct. 5. If this project is under a grant and needs to be acknolwedged, please list the grant statement here. I attest that the student has conducted the work as indicated above and that any required review and approval by institutional regulatory board (IRB/IACUC/IBC) has been obtained. Copies are attached if applicable. I further acknowledge that the student will be presenting this work publicly in competition and I have communicated with the student research regarding any requirements for my review and/or restrictions of what is publicized. Direct Supervisor’s Printed Name Signature Title Institution Date Signed (must be after experimenta- tion) (mm/dd/yy) Address Email/Phone International Rules: Guidelines for Science and Engineering Fairs 2024–2025, societyforscience.org/ISEF Page 35  Qualified Scientist Form (2) May be required for research involving human participants, vertebrate animals, potentially hazardous biological agents, and hazardous substances and devices. Must be completed and signed before the start of student experimentation. Student’s Name(s) Title of Project To be completed by the Qualified Scientist: Scientist Name: Educational Background: Degree(s): Experience/Training as relates to the student’s area of research: Position/Institution: Email/Phone: 1. Have you reviewed the ISEF rules relevant to this project and the science o Yes o No fair ethics statement relevant to this project? 2. Will any of the following be used? a. Human participants o Yes o No b. Vertebrate animals o Yes o No c. Potentially hazardous biological agents (microorganisms, rDNA and o Yes o No tissues, including blood and blood products) d. Hazardous substances and devices o Yes o No 3. Will this study be a sub-set of a larger study? o Yes o No 4. Will you directly supervise the student? o Yes o No To be completed by the Qualified Scientist: To be completed by the Direct Supervisor when the Qualified Scientist cannot directly I certify that I have reviewed and approved the Research Plan/ Project Summary prior to the start of the experimentation. If supervise. the student or Direct Supervisor is not trained in the necessary I certify that I have reviewed the Research Plan/Project procedures, I will ensure her/his training. I will provide Summary and have been trained in the techniques to be used advice and supervision during the research. I have a working by this student, and I will provide direct supervision. knowledge of the techniques to be used by the student in the Research Plan/Project Summary. Direct Supervisor’s Printed Name Qualified Scientist’s Printed Name Experience/Training of Designated Supervisor Signature Date of Approval (mm/dd/yy) Signature Date of Approval (mm/dd/yy) Phone email Page 36 International Rules: Guidelines for Science and Engineering Fairs 2024–2025, societyforscience.org/ISEF  Risk Assessment Form (3) Must be completed before experimentation; recommended for all projects. May be required for projects involving Human Participants, Hazardous Chemicals, Materials or Devices or Potentially Hazardous Biological Agents. Student’s Name(s) Title of Project To be completed by the Student Researcher(s) in collaboration with Direct Supervisor/Qualified Scientist: (All questions must be answered; additional page(s) may be attached.) 1. Identify and assess the risks and hazards involved in this project. 2. a) List all hazardous chemicals, activities or devices to be used; b) identify and list all microorganisms to be used that are exempt from pre-approval (see Potentially Hazardous Biological Agent rules). 3. Describe the safety precautions and procedures that will be used to reduce the risks. 4. Describe the specific disposal procedures that will be used (when applicable). 5. List the source(s) of safety information. To be completed and signed by the Direct Supervisor (or Qualified Scientist, when applicable): I agree with the risk assessment and safety precautions and procedures described above. I certify that I have reviewed the Research Plan/Project Summary and the International Rules, including the science fair ethics statement and will provide direct supervision. Direct Supervisor’s Printed Name Signature Date of Review (mm/dd/yy) Experience/Training as relates to the student’s area of research Position/Institution Phone or email contact information International Rules: Guidelines for Science and Engineering Fairs 2024–2025, societyforscience.org/ISEF Page 37  Human Participants Form (4) Required for all research involving human participants not at a Regulated Research Institution. If at a Regulated Research Institution, use institutional approval forms for documentation of prior review and approval. (IRB approval required before recruitment or data collection.) Student’s Name(s) Title of Project Adult Sponsor Phone/Email MUST BE COMPLETED BY STUDENT RESEARCHER(S) IN COLLABORATION WITH THE ADULT SPONSOR/DIRECT SUPERVISOR/QUALIFIED SCIENTIST: 1. o I have submitted my Research Plan/Project Summary which addresses ALL areas indicated in the Human Participants Section of the Research Plan/Project Summary Instructions. 2. o I have attached any surveys or questionnaires I will be using in my project or other documents provided to human participants. o Any published instrument(s) used was /were legally obtained. 3. o I have attached an informed consent that I would use if required by the IRB. 4. o Yes o No Are you working with a Qualified Scientist? If yes, attach the Qualified Scientist Form 2. BELOW – IRB USE ONLY MUST be completed by Institutional Review Board (IRB) after review of the research plan. All questions must be answered for the approval to be valid. (If not approved, return paperwork to the student with instructions for modifications.) o Approved with Full Committee Review (3 signatures required) and the following conditions: (All 6 must be answered) 1. Risk Level (check one) : o Minimal Risk o More than Minimal Risk (a risk assessment form 3 is required). 2. Qualified Scientist (QS) Required (Form 2): o Yes o No 3. Risk Assessment Required (Form 3): o Yes o No 4. Written Minor Assent required for minor participants: o Yes o No o Not applicable (No minors in this study) 5. Written Parental Permission required for minor participants: o Yes o No o Not applicable (No minors in this study) 6. Written Informed Consent required for participants 18 years or older: o Yes o No o Not applicable (No participants 18 yrs or older in this study) IRB SIGNATURES (All 3 signatures required) None of these individuals may be the adult sponsor, direct supervisor, qualified scientist or related to (e.g., mother, father of) the student (conflict of interest). I attest that I have reviewed the student’s project, that the checkboxes above have been completed to indicate the IRB determination and that I agree with the decisions above. Medical or Mental Health Professional (a psychologist, medical doctor, licensed social worker, licensed clinical professional counselor, physician’s assistant, doctor of pharmacy, or registered nurse) with expertise related to this project. Printed Name Degree/Professional License Signature/Date (prior to experimentation) Email Educator Printed Name Degree/Professional License Signature/Date (prior to experimentation) Email School Administrator Printed Name Degree/Professional License Signature/Date (prior to experimentation) Email Page 38 International Rules: Guidelines for Science and Engineering Fairs 2024–2025, societyforscience.org/ISEF  Human Informed Consent Form Instructions to the Student Researcher(s): An informed consent/assent/permission form should be developed in consultation with the Adult Sponsor, Direct Supervisor or Qualified Scientist. This form is used to provide information to the research participant (or parent/guardian) and to document written informed consent, minor assent, and/or parental permission. When written documentation is required, the researcher keeps the original, signed form. Students may use this sample form or may copy ALL elements of it into a new document. If the form is serving to document parental permission, a copy of any survey or questionnaire must be attached. Student Researcher(s): Title of Project: I am asking for your voluntary participation in my science fair project. Please read the following information about the project. If you would like to participate, please sign in the appropriate area below. Purpose of the project: If you participate, you will be asked to: Time required for participation: Potential Risks of Study: Benefits: How confidentiality will be maintained: If you have any questions about this study, feel free to contact: Adult Sponsor/QS/DS: ____________________________________ Phone/email: _____________________________________________________________ Voluntary Participation: Participation in this study is completely voluntary. If you decide not to participate there will not be negative consequences. Please be aware that if you decide to participate, you may stop participating at any time and you may decide not to answer any specific question. By signing this form I am attesting that I have read and understand the information above and I freely give my consent/ assent to participate or permission for my child to participate. Date Reviewed & Signed: ________________________________________________ Adult Informed Consent or Minor Assent (mm/dd/yy) Research Participant Printed Name: Signature: Parental/Guardian Permission (if applicable) Date Reviewed & Signed: (mm/dd/yy) Parent/Guardian Printed Name: Signature: International Rules: Guidelines for Science and Engineering Fairs 2024–2025, societyforscience.org/ISEF Page 39  Vertebrate Animal Form (5A) Required for all research involving vertebrate animals that is conducted in a school/home/field research site. (SRC approval required before experimentation.) Student’s Name(s) Title of Project To be completed by Student Researcher: 1. Common name (or Genus, species) and number of animals used. 2. Describe completely the housing and husbandry to be provided. Include the cage/pen size, number of animals per cage, environment, bedding, type of food, frequency of food and water, how often animal is observed, etc. Add an additional page as necessary. 3. What will happen to the animals after experimentation? 4. Attach a copy of wildlife licenses or approval forms, as applicable 5. The ISEF Vertebrate Animal Rules require that any death, illness or unexpected weight loss be investigated and documented by a letter from the qualified scientist, direct supervisor or a veterinarian. If applicable, attach this letter with this form when submitting your paperwork to the SRC prior to competition. To be completed by Local or Affiliate Fair Scientific Review Committee (SRC) BEFORE experimentation. Level of Supervision Required for agricultural, behavioral or nutritional studies (select one): o Direct Supervisor REQUIRED. Please have applicable person sign below. o Veterinarian and Direct Supervisor REQUIRED. Please have applicable persons sign below. o Veterinarian, Direct Supervisor and Qualified Scientist REQUIRED. Please have applicable persons sign below and have the Qualified Scientist complete Form (2). The SRC has carefully reviewed this study and finds it is an appropriate study that may be conducted in a non-regulated research site. Local or Affiliate Fair SRC Pre-Approval Signature: SRC Chair Printed Name Signature Date of Approval (must be prior to experimentation) (mm/dd/yy) To be completed by Veterinarian: To be completed by Direct Supervisor or Qualified Scientist when applicable: o I have reviewed this research and animal husbandry with the student before the start of experimentation. o I have reviewed this research and animal husbandry with the student before the start of experimentation and I o I have approved the use and dosages of prescription accept primary responsibility for the care and handling drugs and/or nutritional supplements. of the animals in this project. o I will provide veterinary medical and nursing care in case o I will directly supervise the experiment. of illness or emergency. (Fees may apply.) Printed Name Email/Phone Printed Name Email/Phone Signature Date of Approval (mm/dd/yy) Signature Date of Approval (mm/dd/yy) Page 40 International Rules: Guidelines for Science and Engineering Fairs 2024–2025, societyforscience.org/ISEF  Vertebrate Animal Form (5B) Required for all research involving vertebrate animals that is conducted in at a Regulated Research Institution. (IACUC approval required before experimentation. Form must be completed and signed after experimentation.) Student’s Name(s) Title of Project Title and Protocol Number of IACUC Approved Project: To be completed by Qualified Scientist or Principal Investigator: 1. Species of animals used: Number of animals used: 2. Describe, in detail, the role of the student in this project: animal procedures and related equipment that were involved, oversight provided and safety precautions employed. (Attach extra pages if necessary.) 3. Was there any weight loss or death of any animal? If yes, attach a letter obtained from the qualified scientist, direct supervisor or a veterinarian documenting the situation and the results of the investigation. 4. Did the student’s project also involve the use of tissues? o No o Yes; complete Forms 6A and 6B 5. What laboratory training, including dates, was provided to the student? 6. Attach a copy of the Regulated Research Institution IACUC Approval. A letter from the Qualified Scientist or Principal Investigator is not sufficient. Qualified Scientist/Principal Investigator Printed Name Signature Date (mm/dd/yy) International Rules: Guidelines for Science and Engineering Fairs 2024–2025, societyforscience.org/ISEF Page 41  Potentially Hazardous Biological Agents Risk Assessment Form (6A) Required for research involving microorganisms, rDNA, fresh/frozen tissue (including primary cell lines, human and other primate established cell lines and tissue cultures), blood, blood products and body fluids. SRC/IACUC/IBC approval required before experimentation. Student’s Name(s) Title of Project To be completed by the QUALIFIED SCIENTIST/DIRECT SUPERVISOR in collaboration with the student researcher(s). All questions are applicable and must be answered; additional page(s) may be attached. SECTION 1: PROJECT ASSESSMENT 1. Identify potentially hazardous biological agents to be used in this experiment. Include the strain, source, quantity and the biosafety level risk group of each microorganism. 2. Describe the site of experimentation including the level of biological containment. 3. Describe the procedures that will be used to minimize risk (personal protective equipment, hood type, etc.). 4. What final biosafety level do you recommend for this project given the risk assessment you conducted? 5. Describe the method of disposal of all cultured materials and other potentially hazardous biological agents. If BSL-2 laboratory, include the BSL-2 checklist. SECTION 2: TRAINING 1. What training will the student receive for this project? 2. Experience/training of Direct Supervisor as it relates to the student’s area of research (if applicable). SECTION 3: For ALL CELL LINES, MICROORGANISMS AND TISSUES – To be completed by the QUALIFIED SCIENTIST or Direct Supervisor - Check the appropriate box(es) below: o Experimentation on the microorganisms/cell lines/tissues to be used in this study will NOT be conducted at a Regulated Research Institution, but will be conducted at a (check one) ___BSL-1 or ___BSL-2 laboratory (include a copy of the checklist for BSL-2). [This study has been reviewed by the local SRC and the procedures have been approved prior to experimentation.] o Experimentation on the microorganisms/cell lines/tissues to be used in this study will be conducted at a Regulated Research Institution and was approved by the appropriate institutional board prior to experimentation; institutional approval forms are attached. Origin of cell lines:__________________________________________ Date of IACUC/IBC approval ___________________________ o Experimentation on the microorganisms/cell lines/tissues to be used in this study will be conducted at a Regulated Research Institution, which does not require pre-approval for this type of study. The SRC has seen and approved the research plan and supporting documentation and acknowledges the accuracy of the responses above. CERTIFICATION – To be SIGNED by the QUALIFIED SCIENTIST or Direct Supervisor The QS/DS has seen this project’s research plan and supporting documentation and acknowledges the accuracy of the information provided above. This study has been approved as a (check one) o BSL-1/ o   BSL-2 study, and will be conducted in an appropriate laboratory. QS/DS Printed Name Signature Date of review (mm/dd/yy) SECTION 4: CERTIFICATION – To be completed by the LOCAL or AFFILIATED FAIR SRC The SRC has seen this project’s research plan and supporting documentation and acknowledges the accuracy of the information provided. SRC Printed Name Signature Date of review (mm/dd/yy) Page 42 International Rules: Guidelines for Science and Engineering Fairs 2024–2025, societyforscience.org/ISEF  Human and Vertebrate Animal Tissue Form (6B) Required for research involving fresh/frozen tissue (including primary cell lines, human and other primate established cell lines and tissue cultures), blood, blood products and body fluids. If the research involves living organisms please ensure that the proper human or animal forms are completed. All projects using any tissue listed above must also complete Form 6A. Student’s Name(s) Title of Project To be completed by Student Researcher(s): 1. What vertebrate animal tissue will be used in this study? Check all that apply. o Fresh or frozen tissue sample o Fresh organ or other body part o Blood o Body fluids o Primary cell/tissue cultures o Human or other primate established cell lines 2. Where will the above tissue(s) be obtained? If using an established cell line include source and catalog number. 3. If the tissue will be obtained from a vertebrate animal study conducted at a research institution attach a copy of the IACUC certification with the name of the research institution, the title of the study, the IACUC approval num- ber and a copy of IACUC approval. If human tissues were used, attach a copy of IRB approval. To be completed by the Qualified Scientist or Direct Supervisor: o I verify that the student will work solely with de-identified organs, tissues, cultures or cells that will be supplied to him/her by myself or qualified personnel from the laboratory; and that if vertebrate animals were euthanized they were euthanized for a purpose other than the student’s research. AND/OR o I certify that the blood, blood products, tissues or body fluids in this project will be handled in accordance with the standards and guidance set forth in U.S. Occupational Safety and Health Act, 29CFR, Subpart Z, 1910.1030 - Blood Borne Pathogens. Printed Name Signature Date of Approval (mm/dd/yy) (Must be prior to experimentation.) Title Phone/Email Institution International Rules: Guidelines for Science and Engineering Fairs 2024–2025, societyforscience.org/ISEF Page 43  Continuation/Research Progression Projects Form (7) Required for projects that are a continuation/progression in the same field of study as a previous project. This form must be accompanied by the previous year’s abstract and Research Plan/Project Summary. Student’s Name(s) To be completed by Student Researcher: List all components of the current project that make it new and different from previous research. Components Current Research Project Previous Research Project: Year: ________ 1. Title 2. Change in goal/ purpose/objective 3. Changes in methodology 4. Variable studied 5. Additional changes Attached are: o Abstract and Research Plan/Project Summary, Year _______________ I hereby certify that the above information is correct and that the current year Abstract & Certification and project display board properly reflect work done only in the current year. Student’s Printed Name(s) Signature Date of Signature (mm/dd/yy) Page 44 International Rules: Guidelines for Science and Engineering Fairs 2024–2025, societyforscience.org/ISEF