Immunological Methods - Particle Methods

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12 Questions

In immunochemical methods, what happens when an antigen and a specific antibody come together?

They create a reversible complex

Which technique involves the creation of a complex through antigen-antibody interaction followed by precipitation?

Immunoprecipitation

In immunochemical methods, which technique is primarily used to detect the presence of an antigen or an antibody?

Immunoblotting

Which immunochemical technique involves the use of fluorescence to detect the location of specific antigens in a sample?

Immunofluorescence

Which immunochemical method involves the use of a label attached to either the antigen or antibody for detection?

Immunofluorescence

In which immunochemical method is a precipitate visually detected for analysis?

Immunoelectrophoresis

Which method uses a molecule attached to either the antigen or antibody for detection?

Immunoassays

Which immunochemical method commonly employs immunoglobulins (IgA, IgG, IgM) to bind specifically to antigens or haptens?

Immunoblotting

What type of detector operates on high voltage to measure ionising radiation by producing an avalanche of electrons?

Geiger-Müller (GM) counter detector

In the described method, which type of radiation can the Geiger-Müller (GM) counter detect?

Both alpha and beta radiation

What creates a small ionisation current in the ionising chamber?

Ion pairs migrating due to voltage between electrodes

Which method is suitable for a wide range of energies and preferred for high levels of gamma radiation detection?

Ionising chamber using lower voltage

Study Notes

Immunochemical Methods

  • When an antigen and a specific antibody come together, they form a complex through antigen-antibody interaction, which can lead to precipitation.
  • The technique that involves the creation of a complex through antigen-antibody interaction followed by precipitation is known as precipitation reaction.
  • The primary technique used to detect the presence of an antigen or an antibody is immunoassay.
  • Fluorescence immunoassay involves the use of fluorescence to detect the location of specific antigens in a sample.
  • The technique that involves the use of a label attached to either the antigen or antibody for detection is known as labelled immunoassay.
  • In precipitin reaction, a precipitate is visually detected for analysis.
  • The method that uses a molecule attached to either the antigen or antibody for detection is called labelled immunoassay.
  • The method that commonly employs immunoglobulins (IgA, IgG, IgM) to bind specifically to antigens or haptens is called immunoassay.

Radiation Detection

  • The Geiger-Müller (GM) counter is a type of detector that operates on high voltage to measure ionizing radiation by producing an avalanche of electrons.
  • The Geiger-Müller (GM) counter can detect beta and gamma radiation.
  • Ionizing radiation creates a small ionization current in the ionizing chamber.
  • The Geiger-Müller (GM) counter is suitable for a wide range of energies and is preferred for high levels of gamma radiation detection.

Learn about basic principles of antigen and antibody interaction along with techniques like immunoprecipitation in gel, passive gel immunodiffusion, and immunoelectrophoresis. This quiz is tailored for internal teaching at the Department of Chemical Pathology, NHLS- Wits Medical School.

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