Brucella Diagnostic Methods
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Brucella Diagnostic Methods

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@WonUvite

Questions and Answers

Which specimen is the specimen of choice for Brucellosis diagnosis?

Blood

What is one of the most common sources of laboratory-acquired Brucellosis?

Inhalation of infectious aerosols

Which method can be used for direct detection of Brucellae from clinical specimens?

Real-time PCR

How can neonatal Brucellosis be acquired?

<p>Via ingestion of contaminated breast milk</p> Signup and view all the answers

What are the main prevention and control measures for Brucellosis?

<p>Proper hand hygiene and protective gear for veterinarians</p> Signup and view all the answers

Which specimen is rarely used to isolate Brucella organisms?

<p>Urinary samples</p> Signup and view all the answers

What is the most definitive method for the diagnosis of brucellosis?

<p>Blood culture</p> Signup and view all the answers

Which method is NOT useful for demonstrating Brucella organism in clinical specimens?

<p>Gram staining</p> Signup and view all the answers

How long should subcultures be made on solid media before declaring a culture as negative?

<p>8 weeks</p> Signup and view all the answers

What is a recommended prevention measure for brucellosis?

<p>Pasteurization of milk</p> Signup and view all the answers

Which strain is used for vaccinating cattle against brucellosis?

<p>Brucella abortus strain 16</p> Signup and view all the answers

What is the optimal temperature for incubating blood cultures for brucellosis diagnosis?

<p>37 degrees C</p> Signup and view all the answers

What is the effect of adding bacitracin, polymyxin, and cycloheximide to certain media used for culturing Brucellae?

<p>They make the media selective</p> Signup and view all the answers

How do colonies of Brucellae typically appear on solid media after several days of incubation?

<p>Small, moist, translucent, and glistening</p> Signup and view all the answers

Which of the following statements about Brucellae's resistance is TRUE?

<p>Survives for months in refrigerated milk</p> Signup and view all the answers

Which of the following is a characteristic feature of Brucellae based on biochemical reactions?

<p>Urease negative</p> Signup and view all the answers

How are Brucellae classified into different species?

<p>By lysis from specific Bacteriophage</p> Signup and view all the answers

What is a key feature of Br.melitensis in terms of biotypes?

<p>3 biotypes</p> Signup and view all the answers

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