What is the kinetics of immobilised enzyme?
Understand the Problem
The question is asking about the kinetics of immobilized enzymes, which involves understanding how these enzymes behave and operate when they are attached to a solid support. This includes exploring their reaction rates, substrate concentration impacts, and comparison to free enzymes.
Answer
Immobilization alters enzyme kinetics due to structural changes and restricted active site access.
The kinetics of immobilized enzymes differs from free enzymes, as kinetic constants like Km and Vmax may change due to structural changes and altered access to the active site. Immobilization can reduce enzyme activity due to these factors.
Answer for screen readers
The kinetics of immobilized enzymes differs from free enzymes, as kinetic constants like Km and Vmax may change due to structural changes and altered access to the active site. Immobilization can reduce enzyme activity due to these factors.
More Information
Immobilization can make enzymes more stable and allow them to be reused, which are big benefits in industrial settings despite possible decreases in activity.
Tips
A common mistake is assuming immobilized enzymes will always perform reactions at the same rate as their free counterparts. Rates are often reduced.
Sources
- Kinetics of immobilised enzymes - Water structure and science - water.lsbu.ac.uk
- [PDF] Reaction and Kinetic Studies of Immobilized Enzyme Systems - electrochemsci.org
