Understanding Hematocrit (HCT) and its Measurement
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Questions and Answers

What is the definition of hematocrit (HCT)?

  • The ratio of plasma volume to total blood volume, expressed as a percentage.
  • The percent ratio of red cell volume to total blood volume. (correct)
  • The percentage of white blood cell volume to total blood volume.
  • The concentration of hemoglobin in red blood cells.

Which of the following factors would cause an increased hematocrit (HCT) value?

  • Menstruation
  • Overhydration
  • Anemia
  • Dehydration (correct)

Why is hematocrit typically higher in males than in females?

  • Testosterone increases hematocrit levels, while menstruation decreases it in females. (correct)
  • Males are more prone to overhydration.
  • Males have a lower red blood cell count than females.
  • Females have naturally higher plasma volumes.

A newborn's hematocrit level is found to be 58%. What is the most likely physiological reason for this?

<p>Relative hypoxia (B)</p> Signup and view all the answers

In the procedure for determining hematocrit, what is the purpose of centrifuging the blood sample?

<p>To separate the plasma from the red blood cells. (D)</p> Signup and view all the answers

What is the function of the heparin in the micro-capillary tube used for hematocrit determination?

<p>To prevent the blood from clotting (C)</p> Signup and view all the answers

During hematocrit measurement, if the upper limit of the plasma is set to the 100% line on the Hct scale, what does the zero line represent?

<p>The lower level of red blood cells (B)</p> Signup and view all the answers

A male patient has a hematocrit level of 40%. Based on the normal ranges, how would this be interpreted?

<p>Slightly decreased (D)</p> Signup and view all the answers

What is the primary reason for using a sterilized lancet when collecting a blood sample for hematocrit determination?

<p>To prevent infection (A)</p> Signup and view all the answers

In a patient with polycythemia, what change would you expect to see in their hematocrit level compared to the normal range?

<p>Increased HCT (D)</p> Signup and view all the answers

Flashcards

Hematocrit (HCT)

The percentage of red blood cell volume in relation to the total blood volume.

Packed Cell Volume (PCV)

Another name for Hematocrit, representing the volume of red blood cells after centrifugation.

Normal Hematocrit Values

Male: 44-54%. Female: 37-47%. Newborn: 55-60%.

Factors Affecting HCT

Number of RBCs and volume of plasma.

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Physiological Factors - HCT

Newborns have higher HCT due to relative hypoxia; Males have higher HCT due to testosterone.

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Pathological Factors Affecting HCT

Decreased in Anemia and Overhydration. Increased in Polycythemia and Dehydration.

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HCT Measurement Tools

Alcohol, cotton, sterilized lancet, heparinized micro-capillary tube, crista seal paste, micro-centrifuge, Hct scale/ruler.

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Steps to Determine Hematocrit

Sterilize, prick, fill capillary tube, seal one end, centrifuge, read Hct on scale.

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Appearance After Centrifugation

Packed RBCs at the bottom, plasma above.

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Comment on HCT Result

Compare the result with a reference range

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Study Notes

  • Hematocrit (HCT) is also known as Packed Cell Volume (PCV)
  • It is the percent ratio of red cell volume to total blood volume.
  • Hct = (RBCs volume / Total blood volume) x 100
  • Normal HCT values for males: 44-54%
  • Normal HCT values for females: 37-47%
  • Hct is higher in males than females due to male testosterone and menstruation in females.
  • Normal HCT values for newborns: 55-60%, due to relative hypoxia

Factors Affecting Hematocrit Value:

  • Number of RBCs can affect the hematocrit
  • Volume of plasma is also a factor

Physiological Factors

  • Age: Hct is high in newborns
  • Sex: Hct is more in males
  • High altitude: Due to relative hypoxia

Pathological Factors

  • Anemia and overhydration decrease Hct
  • Polycythemia and dehydration increase Hct

Tools for Measuring Hematocrit

  • Alcohol
  • Cotton
  • Sterilized Lancet
  • Heparinized micro-capillary tube (red circle)
  • Crista seal paste or wax
  • Micro-centrifuge
  • Hct scale or Hct ruler

Steps for Measuring Hematocrit

  • Sterilize the thumb with alcohol, let it dry, and prick it with sterilized lancet
  • Fill the capillary tube (or 3/4 of its length) with oozing blood, which is withdrawn by capillary attraction (capillarity).
  • Seal one end of the tube with provided wax.
  • Centrifuge the tube at 3000 revolutions per minute for 3 minutes.
  • The RBCs will pack together at the bottom of the tube, while plasma remains above
  • Place the lower level of RBCs on the zero line of the Hct scale and the upper limit of clear plasma on the 100% line.
  • Read the Hct directly on the scale at the upper level of RBCs.

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Description

Explore hematocrit (HCT), also known as packed cell volume (PCV), which represents the percentage of red cell volume in total blood volume. Learn about normal HCT values for males, females, and newborns, along with factors affecting HCT levels, including physiological and pathological conditions. Discover the tools and steps involved in accurately measuring hematocrit.

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