Hematocrit Determination Quiz

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Questions and Answers

What is another term for hematocrit?

Packed cell volume (PCV) (correct)

Erythrocyte volume fraction (EVF)

Microhematocrit reader

Red blood cell indices

In which unit is hematocrit reported?

Grams (g)

Percent (%) (correct)

Liters (L)

Milliliters (mL)

What is the main advantage of hematocrit determination over RBC count?

Less prone to errors

Provides information on white blood cell count

More useful in detecting cases of anemia (correct)

Faster to perform

Which of the following materials is NOT used in the Adam's microhematocrit method?

Microhematocrit reader Signup and view all the answers

What is the purpose of sealing one end of the capillary tube with sealing clay in the microhematocrit method?

To prevent blood spillage Signup and view all the answers

At what speed is the blood centrifuged in the microhematocrit method?

10,000 rpm Signup and view all the answers

When is a heparinized (red) capillary tube used in the Adam's microhematocrit method?

If the blood is from a skin puncture Signup and view all the answers

What is the volume of blood filled in the capillary tube in the Adam's microhematocrit method?

Around 3/4 of the capillary tube Signup and view all the answers

Which of the following is an objective of hematocrit determination?

Correlate hematocrit levels with diseases Signup and view all the answers

What can be computed manually from the values of RBC count, hemoglobin level, and hematocrit level?

Red blood cell indices Signup and view all the answers

What is the alternative name for cell volume percent (CV%)?

Volume percent (vol%) Signup and view all the answers

Which of the following is a material used in the Adam's microhematocrit method?

Microhematocrit centrifuge Signup and view all the answers

What is the purpose of preparing a blood smear in the laboratory?

To create a thin, monolayer of cells for microscopic examination Signup and view all the answers

What is the ideal thickness of a blood smear, and how is it achieved?

The ideal thickness is achieved by spreading the blood evenly to create a monolayer Signup and view all the answers

What are the common errors of artifacts that can occur during blood smear preparation, and how can they be minimized or corrected?

Common errors include uneven spreading and overlapping cells; they can be minimized by practicing proper technique Signup and view all the answers

How does the choice of anticoagulant affect the quality of a blood smear, and why is this important to consider?

The choice of anticoagulant can affect cell morphology and staining quality; it is important for accurate microscopic examination Signup and view all the answers

Describe the quality control measures that should be in place to ensure the accuracy and consistency of blood smear preparation.

Regular calibration of equipment and standardization of procedures Signup and view all the answers

What is the most appropriate specimen for smear preparation?

Anticoagulated blood Signup and view all the answers

Which method of smear preparation employs one slide for the smear and another slide as the spreader?

Two-slide or Wedge method Signup and view all the answers

What angle should the spreader slide be positioned at in the Two-slide or Wedge method?

About 30°-45° Signup and view all the answers

What is a characteristic of a good smear?

It must have a smooth and an even surface, free from ridges, waves, or holes. Signup and view all the answers

What is the name of the method that utilizes two cover glasses for smear preparation?

Two-Cover Slip or Ehrlich's Two-Coverglass Method Signup and view all the answers

What is the main advantage of the Two-Cover Slip method over the Two-slide method?

It allows for a thinner and more uniform smear Signup and view all the answers

What should be the coverage of the smear on the slide according to the criteria of a good smear?

⅔ or ¾ of the slide Signup and view all the answers

What is the purpose of using anticoagulated blood for smear preparation?

To prevent blood from clotting Signup and view all the answers

What is the main objective of smear preparation?

To execute the proper way of preparing blood smears Signup and view all the answers

What is the characteristic of a good smear according to the criteria?

It must have a feathery edge Signup and view all the answers

What is the most popular method of smear preparation?

Two-slide or Wedge method Signup and view all the answers

Study Notes

Hematocrit Overview

Another term for hematocrit is packed cell volume (PCV).
Hematocrit is reported in percentage (%).
Main advantage of hematocrit determination over RBC count is providing a quick assessment of blood's capacity to carry oxygen, which reflects the overall blood volume.

Adam's Microhematocrit Method

Materials NOT used in the Adam's microhematocrit method include sodium citrate as an anticoagulant.
Sealing one end of the capillary tube with sealing clay prevents blood leakage and minimizes sample contamination.
Blood is centrifuged at a speed of around 10,000 to 12,000 RPM in the microhematocrit method.
A heparinized (red) capillary tube is used when the blood sample is not mixed with other anticoagulants or when a heparin anticoagulation is preferred.
The volume of blood filled in the capillary tube in the Adam's microhematocrit method is typically approximately 75-100 mm.

Objectives and Calculations

Objective of hematocrit determination includes analyzing anemia and assessing overall red blood cell mass.
From the values of RBC count, hemoglobin level, and hematocrit level, mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC) can be computed.

Blood Smear Preparation

Alternative name for cell volume percent (CV%) is hematocrit.
Purpose of preparing a blood smear is to examine cell morphology and diagnose conditions such as infections and anemia.
Ideal thickness of a blood smear is thin enough to see individual cells; achieved by rapid spreading with a slide.
Common errors during blood smear preparation include thick smears or insufficient spreading; minimize by ensuring proper technique and angle.

Anticoagulant Influence

Choice of anticoagulant affects blood smear quality; EDTA is most commonly used as it preserves morphology, important for accurate diagnosis.

Quality Control Measures

Quality control measures for blood smear preparation include calibration of equipment, using the same anticoagulant consistently, and training personnel.
The most appropriate specimen for smear preparation is fresh, anticoagulated capillary blood.

Methods of Preparation

The Two-slide or Wedge method involves one slide for the smear and another as the spreader slide.
The spreader slide should be positioned at an angle of approximately 30-45° in the Two-slide method.
A characteristic of a good smear includes a feathered edge allowing for optimal cell evaluation.
The method utilizing two cover glasses for smear preparation is called the Two-Cover Slip method.
Main advantage of the Two-Cover Slip method over the Two-slide method is that it provides consistent thickness and minimizes air exposure.
The coverage of the smear on the slide should be about one-half to two-thirds of the slide according to good smear criteria.
Purpose of using anticoagulated blood for smear preparation is to prevent clotting and ensure uniform cell distribution throughout the smear.
Main objective of smear preparation is to visualize blood cells effectively for diagnostic purposes.
Good smear characteristics include being uniform, covering the slide adequately, and showing a clear separation of cells.
The most popular method of smear preparation is the Wedge method.

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Description

Test your knowledge of hematocrit determination with this laboratory manual quiz. Learn about packed red blood cells, erythrocyte volume fraction, and how to calculate and interpret hematocrit results.