Haematocrit (HCT) or Packed Cell Volume (PCV) Lab Test

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Questions and Answers

What is the principle behind determining the packed cell volume (PCV) or hematocrit (HCT)?

  • The R.B.Cs are counted under a microscope and then calculated as a percentage of total blood volume.
  • The R.B.Cs are separated from plasma by a chemical reaction and then measured.
  • The R.B.Cs are packed towards the bottom of the tube by the centrifuge force and reading of the pack cells in percentage. (correct)
  • The R.B.Cs are weighed and then converted to a percentage of total blood volume.

What is the purpose of performing a hematocrit (HCT) or packed cell volume (PCV) test?

  • To determine the size and shape of red blood cells.
  • To diagnose anaemia and polycythaemia, and it is included in the complete blood count (CBC). (correct)
  • To assess the levels of white blood cells in the blood.
  • To measure the oxygen-carrying capacity of red blood cells.

What is used to seal the capillary tube after collecting the blood for hematocrit (HCT) or packed cell volume (PCV) test?

  • Adhesive tape
  • Rubber stopper
  • Clay (correct)
  • Wax

How is the value determined using a microhematocrit reader?

<p>By holding the tube against the scale so that the bottom of the red cell is matched with the 0 (zero) line of the card. (D)</p> Signup and view all the answers

At what speed and for how long is centrifugation done for a microhematocrit test?

<p>$10000$ rpm for 5 minutes (C)</p> Signup and view all the answers

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Study Notes

Determining Packed Cell Volume (PCV) or Hematocrit (HCT)

  • The principle behind determining PCV or HCT is to measure the proportion of red blood cells in the blood.
  • The purpose of performing a PCV or HCT test is to evaluate the proportion of red blood cells in the blood, which is essential in diagnosing and monitoring anemia, polycythemia, and blood loss.

Sample Preparation

  • A capillary tube is used to collect the blood sample.
  • The capillary tube is sealed with clay or a sealant after collecting the blood.

Measuring PCV or HCT using a Microhematocrit Reader

  • The microhematocrit reader is used to determine the PCV or HCT value.
  • The value is determined by reading the proportion of red blood cells from the separator line in the microhematocrit reader.

Centrifugation

  • Centrifugation is done at a speed of approximately 10,000-12,000 rpm for 5 minutes to separate the blood components.
  • The centrifugation process is necessary to pack the red blood cells at the bottom of the capillary tube, allowing for accurate measurement of PCV or HCT.

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