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Questions and Answers
The hematocrit and PCV are the same measurement.
The hematocrit and PCV are the same measurement.
True (A)
The hematocrit is measured in units of $mL$
The hematocrit is measured in units of $mL$
False (B)
A PCV of 45% indicates that 45 mL of blood is made up of red blood cells.
A PCV of 45% indicates that 45 mL of blood is made up of red blood cells.
False (B)
A higher PCV is indicative of anemia.
A higher PCV is indicative of anemia.
EVF is a measurement of the volume of white blood cells within blood.
EVF is a measurement of the volume of white blood cells within blood.
The Microhaematocrit Method requires a large amount of blood.
The Microhaematocrit Method requires a large amount of blood.
The Microhaematocrit Method utilizes a centrifuge, specifically a Microhaematocrit centrifuge.
The Microhaematocrit Method utilizes a centrifuge, specifically a Microhaematocrit centrifuge.
A lancet is used to collect blood in this method.
A lancet is used to collect blood in this method.
The Microhaematocrit Method is a commonly used method for determining hematocrit.
The Microhaematocrit Method is a commonly used method for determining hematocrit.
The Microhaematocrit Method typically requires a heparinized capillary tube with a blue ring at the end.
The Microhaematocrit Method typically requires a heparinized capillary tube with a blue ring at the end.
The ESR scale runs from 100 to 0, with higher values representing higher sedimentation rates.
The ESR scale runs from 100 to 0, with higher values representing higher sedimentation rates.
The PCV (Packed Cell Volume) scale runs from 0 to 100, with lower values indicating a lower percentage of red blood cells in the blood.
The PCV (Packed Cell Volume) scale runs from 0 to 100, with lower values indicating a lower percentage of red blood cells in the blood.
The 4-Microhaematocrit reader can produce a relative centrifugal force of 10,000 to 15,000 grams.
The 4-Microhaematocrit reader can produce a relative centrifugal force of 10,000 to 15,000 grams.
A microhematocrit reader is used to measure the volume of red blood cells in a sample.
A microhematocrit reader is used to measure the volume of red blood cells in a sample.
The ESR and PCV are both calculated using a centrifuge, which spins the blood at a high speed.
The ESR and PCV are both calculated using a centrifuge, which spins the blood at a high speed.
The relative centrifugal force (RCF) is measured in units of kilograms.
The relative centrifugal force (RCF) is measured in units of kilograms.
Aseptic conditions are required for ESR and PCV blood draws to prevent the introduction of bacteria into the blood stream.
Aseptic conditions are required for ESR and PCV blood draws to prevent the introduction of bacteria into the blood stream.
When the ESR is high, the blood will settle faster due to the higher concentration of red blood cells.
When the ESR is high, the blood will settle faster due to the higher concentration of red blood cells.
The 4-Microhaematocrit reader is capable of producing a relative centrifugal force of 10,000 to 15,000 $kg$
The 4-Microhaematocrit reader is capable of producing a relative centrifugal force of 10,000 to 15,000 $kg$
The 4-Microhaematocrit reader can produce a relative centrifugal force of 10,000 to 15,000 $g$
The 4-Microhaematocrit reader can produce a relative centrifugal force of 10,000 to 15,000 $g$
A centrifuge spins at a rate of 3000 revolutions per minute.
A centrifuge spins at a rate of 3000 revolutions per minute.
The centrifuge should be stopped after 30 minutes.
The centrifuge should be stopped after 30 minutes.
A centrifuge is a piece of equipment that uses the force of gravity to separate substances.
A centrifuge is a piece of equipment that uses the force of gravity to separate substances.
The time required to centrifuge a sample is dependent on the size and density of the sample.
The time required to centrifuge a sample is dependent on the size and density of the sample.
A centrifuge can only be used with blood samples.
A centrifuge can only be used with blood samples.
A PCV of 40% in a male adult would be considered anemic.
A PCV of 40% in a male adult would be considered anemic.
If a male adult has a PCV of 47%, they are within the normal range for PCV.
If a male adult has a PCV of 47%, they are within the normal range for PCV.
The normal range for PCV in adult males is 40-52%.
The normal range for PCV in adult males is 40-52%.
If a male adult has a PCV of 50%, they are anemic.
If a male adult has a PCV of 50%, they are anemic.
A PCV of 45% indicates that 45% of the blood is made up of red blood cells.
A PCV of 45% indicates that 45% of the blood is made up of red blood cells.
Flashcards
Hematocrit
Hematocrit
The proportion of blood volume occupied by red blood cells.
Packed Cell Volume (PCV)
Packed Cell Volume (PCV)
Another name for hematocrit, indicating the volume of red blood cells.
Erythrocyte Volume Fraction (EVF)
Erythrocyte Volume Fraction (EVF)
A term synonymous with hematocrit, representing red blood cell fraction.
Measuring PCV
Measuring PCV
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Importance of Hematocrit
Importance of Hematocrit
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Microhaematocrit Method
Microhaematocrit Method
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Heparinized Capillary Tubes
Heparinized Capillary Tubes
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Microhaematocrit Centrifuge
Microhaematocrit Centrifuge
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Microhaematocrit Reader
Microhaematocrit Reader
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Modeling Clay Sealant
Modeling Clay Sealant
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Centrifugal Force
Centrifugal Force
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Relative Centrifugal Force
Relative Centrifugal Force
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Centrifugal Force Range
Centrifugal Force Range
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Blood Sample Preparation
Blood Sample Preparation
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ESR Scale
ESR Scale
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PCV
PCV
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Aseptic Procedure
Aseptic Procedure
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Venous Blood Sample
Venous Blood Sample
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Centrifuge
Centrifuge
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3000 rpm
3000 rpm
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30 minutes
30 minutes
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Stop centrifuge
Stop centrifuge
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Separation process
Separation process
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Wintrobe Tube
Wintrobe Tube
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Normal PCV Range for Men
Normal PCV Range for Men
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PCV Below Normal
PCV Below Normal
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Anemia Definition
Anemia Definition
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Reading PCV
Reading PCV
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Study Notes
PCV Packed Cell Volume Test
- PCV measures the volume of red blood cells in 100mL (dL) of blood.
- Hematocrit is another name for PCV.
- It's the percentage of red blood cells in a blood sample after centrifugation.
- PCV is also referred to as erythrocyte volume fraction (EVF).
Low Hematocrit Levels
- Low hemoglobin levels can be due to anemia.
- Hemolysis can also cause low hematocrit.
- Nutritional deficiencies can also result in low hematocrit.
- Bleeding can cause lower hematocrit levels.
- Pregnancy also contributes to low hematocrit levels.
High Hematocrit Levels
- Polycythemia occurs with a high hematocrit.
- Heart or kidney problems can cause high hematocrit levels.
- Using anabolic steroids can result in high hematocrit levels.
- Dehydration also leads to high hematocrit.
- Diarrhea can contribute to high hematocrit.
- Lung problems are associated with high hematocrit.
- Burns can cause high hematocrit values.
- Smoking and high altitudes are other factors contributing to high hematocrit levels.
Hematocrit Methods
- A-Microhaematocrit method: Requires less blood and time, commonly used.
- Materials: Cotton, lancet, heparinized capillary tubes (red label), modeling clay sealant, microhaematocrit centrifuge, microhaematocrit reader.
- B-Macrohaematocrit or Wintrobe Method: Less commonly used today due to longer time and higher blood requirement.
- Materials: Wintrobe tube, Wintrobe pipette rack, macrohaematocrit centrifuge, disposable Pasteur pipette, EDTA as anticoagulant.
Procedure for Microhaematocrit Method
- Clean the finger with 70% alcohol and let it dry.
- Prick the finger with the lancet near the tip. Ensure free blood flow or use wrist pressure if no flow.
- Put the tip of a capillary tube onto the blood drop on the finger.
- Seal the tube.
- Spin the tube in the centrifuge for 5 minutes at 10,000 rpm.
Procedure for Macrohaematocrit Method
- Under aseptic conditions, collect venous blood samples.
- Mix blood with EDTA vial by inversion.
- Fill the Wintrobe tube with the help of a Pasteur pipette up to the 10 cm mark.
- Place the Wintrobe tube in the centrifuge, balanced with another tube containing water on the opposite side.
- Turn the centrifuge on low speed, then gradually increase speed.
- Centrifuge for 30 minutes at 3000 rpm.
- Stop the centrifuge.
- Take out the Wintrobe tube and read the results directly from the gradation.
PCV Normal Values
- Adult males: 44-51%
- Less than 44% indicates anemia.
- More than 51% indicates polycythemia.
- Adult females: 38-48%
- Less than 38% indicates anemia.
- More than 48% indicates polycythemia.
- Late pregnancy: 23-37%
- At birth: 44-62%
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