Hematocrit and PCV Measurement Methods
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Questions and Answers

The hematocrit and PCV are the same measurement.

True (A)

The hematocrit is measured in units of $mL$

False (B)

A PCV of 45% indicates that 45 mL of blood is made up of red blood cells.

False (B)

A higher PCV is indicative of anemia.

<p>False (B)</p> Signup and view all the answers

EVF is a measurement of the volume of white blood cells within blood.

<p>False (B)</p> Signup and view all the answers

The Microhaematocrit Method requires a large amount of blood.

<p>False (B)</p> Signup and view all the answers

The Microhaematocrit Method utilizes a centrifuge, specifically a Microhaematocrit centrifuge.

<p>True (A)</p> Signup and view all the answers

A lancet is used to collect blood in this method.

<p>True (A)</p> Signup and view all the answers

The Microhaematocrit Method is a commonly used method for determining hematocrit.

<p>True (A)</p> Signup and view all the answers

The Microhaematocrit Method typically requires a heparinized capillary tube with a blue ring at the end.

<p>False (B)</p> Signup and view all the answers

The ESR scale runs from 100 to 0, with higher values representing higher sedimentation rates.

<p>True (A)</p> Signup and view all the answers

The PCV (Packed Cell Volume) scale runs from 0 to 100, with lower values indicating a lower percentage of red blood cells in the blood.

<p>True (A)</p> Signup and view all the answers

The 4-Microhaematocrit reader can produce a relative centrifugal force of 10,000 to 15,000 grams.

<p>True (A)</p> Signup and view all the answers

A microhematocrit reader is used to measure the volume of red blood cells in a sample.

<p>True (A)</p> Signup and view all the answers

The ESR and PCV are both calculated using a centrifuge, which spins the blood at a high speed.

<p>True (A)</p> Signup and view all the answers

The relative centrifugal force (RCF) is measured in units of kilograms.

<p>False (B)</p> Signup and view all the answers

Aseptic conditions are required for ESR and PCV blood draws to prevent the introduction of bacteria into the blood stream.

<p>True (A)</p> Signup and view all the answers

When the ESR is high, the blood will settle faster due to the higher concentration of red blood cells.

<p>False (B)</p> Signup and view all the answers

The 4-Microhaematocrit reader is capable of producing a relative centrifugal force of 10,000 to 15,000 $kg$

<p>False (B)</p> Signup and view all the answers

The 4-Microhaematocrit reader can produce a relative centrifugal force of 10,000 to 15,000 $g$

<p>True (A)</p> Signup and view all the answers

A centrifuge spins at a rate of 3000 revolutions per minute.

<p>True (A)</p> Signup and view all the answers

The centrifuge should be stopped after 30 minutes.

<p>True (A)</p> Signup and view all the answers

A centrifuge is a piece of equipment that uses the force of gravity to separate substances.

<p>False (B)</p> Signup and view all the answers

The time required to centrifuge a sample is dependent on the size and density of the sample.

<p>True (A)</p> Signup and view all the answers

A centrifuge can only be used with blood samples.

<p>False (B)</p> Signup and view all the answers

A PCV of 40% in a male adult would be considered anemic.

<p>True (A)</p> Signup and view all the answers

If a male adult has a PCV of 47%, they are within the normal range for PCV.

<p>True (A)</p> Signup and view all the answers

The normal range for PCV in adult males is 40-52%.

<p>False (B)</p> Signup and view all the answers

If a male adult has a PCV of 50%, they are anemic.

<p>False (B)</p> Signup and view all the answers

A PCV of 45% indicates that 45% of the blood is made up of red blood cells.

<p>True (A)</p> Signup and view all the answers

Flashcards

Hematocrit

The proportion of blood volume occupied by red blood cells.

Packed Cell Volume (PCV)

Another name for hematocrit, indicating the volume of red blood cells.

Erythrocyte Volume Fraction (EVF)

A term synonymous with hematocrit, representing red blood cell fraction.

Measuring PCV

PCV is expressed as the volume of red blood cells per 100mL of blood.

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Importance of Hematocrit

Hematocrit indicates oxygen-carrying capacity and hydration status.

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Microhaematocrit Method

A technique to determine hematocrit using less blood and time.

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Heparinized Capillary Tubes

Tubes containing heparin to prevent blood clotting during hematocrit tests.

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Microhaematocrit Centrifuge

A centrifuge designed specifically for spinning microhaematocrit tubes to separate blood components.

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Microhaematocrit Reader

Device used to measure hematocrit levels after centrifugation of blood samples.

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Modeling Clay Sealant

Material used to seal the ends of microhaematocrit tubes to prevent leakage during centrifugation.

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Centrifugal Force

The force that pushes objects away from the center of rotation.

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Relative Centrifugal Force

Centrifugal force experienced relative to the gravity on Earth, typically measured in g's.

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Centrifugal Force Range

The specific range of centrifugal forces produced, typically between 10,000 to 15,000 g.

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Blood Sample Preparation

The procedure of cleaning and preparing a finger for blood testing.

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ESR Scale

A measurement scale used for Erythrocyte Sedimentation Rate, ranging 100 upwards.

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PCV

Packed Cell Volume, a measure used to determine hematocrit levels in blood.

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Aseptic Procedure

A method to collect blood samples without contamination.

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Venous Blood Sample

A blood sample taken from a vein for testing.

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Centrifuge

A machine that separates substances by spinning them at high speeds.

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3000 rpm

Revolutions per minute; a measure of how fast the centrifuge spins.

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30 minutes

The duration for which the centrifuge operates in this process.

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Stop centrifuge

Cease the operation of the centrifuge after the designated time.

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Separation process

The outcome of using a centrifuge to divide different components in a sample.

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Wintrobe Tube

A device used to measure PCV by separating blood components.

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Normal PCV Range for Men

The normal PCV range in adult men is 44-51%.

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PCV Below Normal

If PCV is less than 44% in males, it indicates anemia.

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Anemia Definition

A condition where the PCV is low, signifying insufficient red blood cells.

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Reading PCV

PCV readings are taken directly off the Wintrobe tube graduation.

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Study Notes

PCV Packed Cell Volume Test

  • PCV measures the volume of red blood cells in 100mL (dL) of blood.
  • Hematocrit is another name for PCV.
  • It's the percentage of red blood cells in a blood sample after centrifugation.
  • PCV is also referred to as erythrocyte volume fraction (EVF).

Low Hematocrit Levels

  • Low hemoglobin levels can be due to anemia.
  • Hemolysis can also cause low hematocrit.
  • Nutritional deficiencies can also result in low hematocrit.
  • Bleeding can cause lower hematocrit levels.
  • Pregnancy also contributes to low hematocrit levels.

High Hematocrit Levels

  • Polycythemia occurs with a high hematocrit.
  • Heart or kidney problems can cause high hematocrit levels.
  • Using anabolic steroids can result in high hematocrit levels.
  • Dehydration also leads to high hematocrit.
  • Diarrhea can contribute to high hematocrit.
  • Lung problems are associated with high hematocrit.
  • Burns can cause high hematocrit values.
  • Smoking and high altitudes are other factors contributing to high hematocrit levels.

Hematocrit Methods

  • A-Microhaematocrit method: Requires less blood and time, commonly used.
    • Materials: Cotton, lancet, heparinized capillary tubes (red label), modeling clay sealant, microhaematocrit centrifuge, microhaematocrit reader.
  • B-Macrohaematocrit or Wintrobe Method: Less commonly used today due to longer time and higher blood requirement.
    • Materials: Wintrobe tube, Wintrobe pipette rack, macrohaematocrit centrifuge, disposable Pasteur pipette, EDTA as anticoagulant.

Procedure for Microhaematocrit Method

  1. Clean the finger with 70% alcohol and let it dry.
  2. Prick the finger with the lancet near the tip. Ensure free blood flow or use wrist pressure if no flow.
  3. Put the tip of a capillary tube onto the blood drop on the finger.
  4. Seal the tube.
  5. Spin the tube in the centrifuge for 5 minutes at 10,000 rpm.

Procedure for Macrohaematocrit Method

  1. Under aseptic conditions, collect venous blood samples.
  2. Mix blood with EDTA vial by inversion.
  3. Fill the Wintrobe tube with the help of a Pasteur pipette up to the 10 cm mark.
  4. Place the Wintrobe tube in the centrifuge, balanced with another tube containing water on the opposite side.
  5. Turn the centrifuge on low speed, then gradually increase speed.
  6. Centrifuge for 30 minutes at 3000 rpm.
  7. Stop the centrifuge.
  8. Take out the Wintrobe tube and read the results directly from the gradation.

PCV Normal Values

  • Adult males: 44-51%
    • Less than 44% indicates anemia.
    • More than 51% indicates polycythemia.
  • Adult females: 38-48%
    • Less than 38% indicates anemia.
    • More than 48% indicates polycythemia.
  • Late pregnancy: 23-37%
  • At birth: 44-62%

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Description

This quiz explores the concepts of hematocrit and packed cell volume (PCV), highlighting their definitions and measurement techniques. Key methods such as the Microhaematocrit Method and relevant tools like centrifuges and lancets are discussed. Test your understanding of these important blood analysis techniques.

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