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Radioimmunoassay: Principle, Uses, and Limitations

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Indirect detection of radiolabeled antigens

Measurement of enzyme activity in antigen-antibody complexes

Direct binding of radiolabeled antigens to antibodies

Competitive binding of radiolabeled and unlabeled antigens to antibodies (correct)

Presence of an antibody

Presence of an antigen (correct)

Radio emission from the antigen-antibody complex

Enzyme activity in antigen-antibody complexes

Specificity (correct)

Enzyme activity measurement

Direct binding of antigens and antibodies

Sensitivity

<p>Antigen-antibody binding</p> Signup and view all the answers

<p>Counting radioactivity in the precipitates</p> Signup and view all the answers

RIA is based on the principle of competitive binding between a radiolabeled antigen and a non-radiolabeled antigen for a limited number of antibody binding sites.
RIA measures the concentration of an antigen (substance) in a sample with very high sensitivity, typically in the picomolar range.
The competitive binding in RIA provides a quantitative measurement of the antigen concentration.
RIA involves an immune reaction between an antigen and its corresponding antibody, where the antibody is immobilized on a solid support (e.g., beads or tubes).
The amount of free radiolabeled antigen is determined in RIA by separating it from the antibody-bound radiolabeled antigen, and then measuring the radioactivity of the free fraction.