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Questions and Answers
What is the principle behind radioimmunoassay (RIA)?
What is the principle behind radioimmunoassay (RIA)?
- Indirect detection of radiolabeled antigens
- Measurement of enzyme activity in antigen-antibody complexes
- Direct binding of radiolabeled antigens to antibodies
- Competitive binding of radiolabeled and unlabeled antigens to antibodies (correct)
What does RIA measure with very high sensitivity?
What does RIA measure with very high sensitivity?
- Presence of an antibody
- Presence of an antigen (correct)
- Radio emission from the antigen-antibody complex
- Enzyme activity in antigen-antibody complexes
What does the competitive binding in RIA provide?
What does the competitive binding in RIA provide?
- Specificity (correct)
- Enzyme activity measurement
- Direct binding of antigens and antibodies
- Sensitivity
What does RIA involve in terms of immune reaction?
What does RIA involve in terms of immune reaction?
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How is the amount of free radiolabeled antigen determined in RIA?
How is the amount of free radiolabeled antigen determined in RIA?
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Study Notes
Radioimmunoassay (RIA)
- RIA is based on the principle of competitive binding between a radiolabeled antigen and a non-radiolabeled antigen for a limited number of antibody binding sites.
- RIA measures the concentration of an antigen (substance) in a sample with very high sensitivity, typically in the picomolar range.
- The competitive binding in RIA provides a quantitative measurement of the antigen concentration.
- RIA involves an immune reaction between an antigen and its corresponding antibody, where the antibody is immobilized on a solid support (e.g., beads or tubes).
- The amount of free radiolabeled antigen is determined in RIA by separating it from the antibody-bound radiolabeled antigen, and then measuring the radioactivity of the free fraction.
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