Lect. 9
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What is a disadvantage of DNA microarrays?

  • They have a small dynamic range for gene expression
  • They can only measure the relative quantities of a few mRNAs
  • They lack sensitivity for genes expressed at low levels (correct)
  • They are unable to detect the presence of specific mRNAs
  • What is a strength of microarrays in gene expression profiling?

  • They allow measurement of the relative quantities of specific mRNAs in multiple samples simultaneously (correct)
  • They can detect all types of gene mutations
  • They provide accurate measurements of protein levels
  • They are cost-effective for large-scale experiments
  • What is the principle of microarray based on?

  • Transcription
  • Translation
  • Hybridization (correct)
  • Replication
  • Microarray technology initially involved spotting random cDNA clones to detect genes with differential expression levels in organisms with extensive DNA sequence information

    <p>False</p> Signup and view all the answers

    SAGE and MPSS are used for the discovery of new mRNA species in organisms where genome information is unavailable

    <p>False</p> Signup and view all the answers

    The amount of target sample binding to the probe present on the spot is not related to the total strength of the signal from a spot in microarray technology

    <p>False</p> Signup and view all the answers

    Microarrays allow the researcher to measure the absolute quantities of specific mRNAs in two or more samples for hundreds or thousands of genes simultaneously

    <p>False</p> Signup and view all the answers

    The production of too many results at a time requires a short time for analysis and is not very complex

    <p>False</p> Signup and view all the answers

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