5 Questions
What is the purpose of the exchange reaction involving PNK?
To label with a radioactive phosphate group
What is the function of PNK in the conversion of 5' dephospho-ribonucleic acid to 5' phospho-ribonucleic acid?
It transfers the phosphate group
What additional activity does PNK have besides phosphorylating nucleic acids?
3' phosphatase activity
What is one major use of PNK in genetic engineering?
Phosphorylation of linkers and adaptors before ligation
Why is PNK used in radio labeling oligonucleotides?
For preparing hybridization probes
Test your knowledge on the composition of recognition sites in genetic engineering, including the specific nucleotide lengths and required components for activity. Learn about the asymmetrical structure and spacer sequences in enzyme recognition sites.
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