Genetic Engineering Recognition Site Composition Quiz
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Questions and Answers

What is the purpose of the exchange reaction involving PNK?

To label with a radioactive phosphate group

What is the function of PNK in the conversion of 5' dephospho-ribonucleic acid to 5' phospho-ribonucleic acid?

It transfers the phosphate group

What additional activity does PNK have besides phosphorylating nucleic acids?

3' phosphatase activity

What is one major use of PNK in genetic engineering?

<p>Phosphorylation of linkers and adaptors before ligation</p> Signup and view all the answers

Why is PNK used in radio labeling oligonucleotides?

<p>For preparing hybridization probes</p> Signup and view all the answers

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