18 Questions
Which method of vaccine production requires large-scale cell culture?
mRNA vaccines
What is the advantage of delivering mRNA vaccines via lipid nanoparticles?
They protect RNA from being broken down in the body
What are knockout mice commonly used for in genetic research?
All of the above
Which company filed the most 'plant' patents?
DuPont-Pioneer
What is the main purpose of gene drives?
To change genetic characteristics
What is the potential risk of releasing gene-drive modified organisms into the environment?
Unintended consequences for public health
What is the key feature of gene drives?
Potential to cause irreversible ecological change
Which type of genetic engineering targets the genes in specific organs and tissues of the body of a single existing person without affecting genes in their eggs or sperm?
Somatic genetic engineering
Which type of genetic engineering targets the genes in eggs, sperm, or very early embryos, and the alterations affect every cell in the body of the resulting individual, and are passed on to all future generations?
Germline genetic engineering
Which country forbids the use of federal funds for human germline editing research?
The US
Which of the following is an application of engineered nuclease (Fokl)?
Editing tumor-infiltrating lymphocytes
Which of the following genome editing tools has the highest specificity compared to ZFNs and TALENs?
Engineered CRISPR
What is the advantage of using CRISPR/Cas9 expression plasmids with codons optimized for human cells?
Higher editing efficiency
Which of the following is NOT a medical application of genome editing?
Editing plant tissue culture
Which technique is used to determine the effect of changing DNA sequence on gene or DNA function?
Site-directed mutagenesis
What is the purpose of annealing synthetic oligonucleotide with desired mutation to target region in site-directed mutagenesis?
To introduce desired mutation into the target region
Which type of mutation can be introduced into a gene of interest using cassette mutagenesis?
All of the above
Which genome editing mechanism uses engineered nucleases to insert, delete, or replace DNA in the genome?
CRISPR-Cas9 system
Test your knowledge on Engineered Nucleases and TALENs gene editing techniques! Learn about their applications, including disabling HIV receptor on T-cells and editing tumor-infiltrating lymphocytes for melanoma treatment. Explore the components and functions of these powerful tools in DNA manipulation.
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