Exploring Engineered Nucleases and TALENs
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Questions and Answers

Which method of vaccine production requires large-scale cell culture?

  • mRNA vaccines (correct)
  • Vector-based vaccines
  • Protein-based vaccines
  • Whole virus-based vaccines
  • What is the advantage of delivering mRNA vaccines via lipid nanoparticles?

  • They increase protein production in cells
  • They trigger inflammatory reactions
  • They protect RNA from being broken down in the body (correct)
  • They require sophisticated carrier lipid systems
  • What are knockout mice commonly used for in genetic research?

  • Investigating the nature of genetic diseases
  • Testing the efficacy of different types of treatment
  • Developing effective gene therapies
  • All of the above (correct)
  • Which company filed the most 'plant' patents?

    <p>DuPont-Pioneer</p> Signup and view all the answers

    What is the main purpose of gene drives?

    <p>To change genetic characteristics</p> Signup and view all the answers

    What is the potential risk of releasing gene-drive modified organisms into the environment?

    <p>Unintended consequences for public health</p> Signup and view all the answers

    What is the key feature of gene drives?

    <p>Potential to cause irreversible ecological change</p> Signup and view all the answers

    Which type of genetic engineering targets the genes in specific organs and tissues of the body of a single existing person without affecting genes in their eggs or sperm?

    <p>Somatic genetic engineering</p> Signup and view all the answers

    Which type of genetic engineering targets the genes in eggs, sperm, or very early embryos, and the alterations affect every cell in the body of the resulting individual, and are passed on to all future generations?

    <p>Germline genetic engineering</p> Signup and view all the answers

    Which country forbids the use of federal funds for human germline editing research?

    <p>The US</p> Signup and view all the answers

    Which of the following is an application of engineered nuclease (Fokl)?

    <p>Editing tumor-infiltrating lymphocytes</p> Signup and view all the answers

    Which of the following genome editing tools has the highest specificity compared to ZFNs and TALENs?

    <p>Engineered CRISPR</p> Signup and view all the answers

    What is the advantage of using CRISPR/Cas9 expression plasmids with codons optimized for human cells?

    <p>Higher editing efficiency</p> Signup and view all the answers

    Which of the following is NOT a medical application of genome editing?

    <p>Editing plant tissue culture</p> Signup and view all the answers

    Which technique is used to determine the effect of changing DNA sequence on gene or DNA function?

    <p>Site-directed mutagenesis</p> Signup and view all the answers

    What is the purpose of annealing synthetic oligonucleotide with desired mutation to target region in site-directed mutagenesis?

    <p>To introduce desired mutation into the target region</p> Signup and view all the answers

    Which type of mutation can be introduced into a gene of interest using cassette mutagenesis?

    <p>All of the above</p> Signup and view all the answers

    Which genome editing mechanism uses engineered nucleases to insert, delete, or replace DNA in the genome?

    <p>CRISPR-Cas9 system</p> Signup and view all the answers

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