Lecture 1 - Basics of Biotechnology.pdf

UPSC Prelims Science & Tech Class 1 Orientation, Biotechnology Basics First Class : Science and Technology for UPSC Prelims 2023 Orientation : Basic details about the course What you can expect from me What I expect from you Doubts / Queries and Everything else...

UPSC Prelims Science & Tech Class 1 Orientation, Biotechnology Basics First Class : Science and Technology for UPSC Prelims 2023 Orientation : Basic details about the course What you can expect from me What I expect from you Doubts / Queries and Everything else Academic : Basics of Biotechnology Some PYQs Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Orientation : Teaching methodology and notes Teaching methodology : Slides on the digital board Static First, for conceptual clarity. Current Affairs integration PYQs : 2013 to 2022 Notes : Slides will be shared, without annotation One Pager Notes at the end of the class Both of these can be downloaded from the portal. Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More  Orientation : Class Schedule 23rd March Thursday Space and Défense 1 Date Day Subject 24th March Friday Défense 2 15th March Wednesday Orientation and Biotechnology Basics Emerging Technology and 25th March Saturday Computers 1 16th March Thursday Basic Biology: Cells and Genetics Principles of Biotech and their 27th March Monday Emerging Tech and Computers 2 17th March Friday applications 18th March Saturday Biotechnology Current Affairs 28th March Tuesday Energy : Basics 20th March Monday Health Issues 29th March Wednesday IPR and Role of Scientists 21st March Tuesday Nanotechnology 30th March Thursday Reserve Class 22nd March Wednesday Space Technology 31st March Friday Reserve Class Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Orientation : Class timing Classes will be held from Monday to Saturday. No Class on Sunday : Your time to revise Class timing : 12 NOON Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Orientation : Doubts and Queries Answering doubts is extremely important. Completing the course on time is also very important. In the live class, I will ask you to participate. Put all your comments / queries / suggestions in the discussions board below. Whatever I can address during the class, I will. Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Orientation : Doubts and Queries Remaining questions will be dealt with on the discussion board, and you will get the answers to your questions. Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More What you can expect from me Firstly, Thank you for placing your trust in me. I will give my best here. Coverage of all the static themes in science and technology. Barring any force majeure, the course will be finished on time and as per schedule. Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More  What you can expect from me Coverage of All Current Affairs : You DO NOT NEED TO READ PT 365 : I will cover the PT-365 of past 2 years. Coverage of ALL PYQs from 2013 to 2022 Relevant Content : No mushrooming of Content. Only what is relevant. Our target here is to get a good percentage of the questions right. Our goal is not to become a scientist, but to get 100+ marks in prelims. Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More  Utilizing this course effectively 15th March to 31st March : Science and Technology 3rd April to 20th April : Environment Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Utilizing this course effectively Attend the courses live Spend 2-3 Hours here, after that DO NOT take a break. Spend at least 15 minutes revising what we have learnt from the slides and one pager notes. Next morning : Revise the previous session before coming here. Out of your 8 hours of daily study time, I’m asking for 4 hours. Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More What I expect from you Work hard and listen effectively. Use Active Recall to Revise. This is what your ideal schedule looks like : Revise yesterday’s class in the morning 12pm onwards : Attend the class here After the class : Revise from the one pager for 15-20 minutes. Post lunch : Study a different subject / give test Sunday : Revise whatever has been taught the entire week. Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More What I expect from you Live attendance of class Regularly Proper timely revision, daily and on Sunday Result : From today till 20th April, you will be done with Science and tech and Environment. Your 2 biggest fears will be sorted. If you follow the plan, you will have a set of one pagers which you will have revised from. 50-60 pages with relevant content that you can effectively revise in one day. Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More LET’S GET STARTED! First topic to cover : Biotechnology Basics. Some of this was covered in the demo class, but I will start assuming that you have not seen any of it. So if you have not read any science/ environment before today, don’t worry. Leave it to me. Just stay attentive. First we will study biotechnology. Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Now, I will show you a video of HIV infecting a Cell Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Let’s Solve Some Previous Year Questions! Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Previous Year Questions: 2016 Q. In the context of the developments in bioinformatics, the term ʻtranscriptomeʼ, sometimes seen in the news, refer to - a) a range of enzymes used in genome editing b) the full range of mRNA molecules expressed by an organism c) the description of the mechanism of gene expression d) A mechanism of genetic mutation taking place in cells Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Answer: B The transcriptome is the set of all RNA molecules in one cell or a population of cells. It is sometimes used to refer to all RNAs, or just mRNA, depending on the particular experiment. It differs from the exome in that it includes only those RNA molecules found in a specified cell population, and usually includes the amount or concentration of each RNA molecule in addition to the molecular identities Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Previous Year Questions: 2016 Q.Which of the following statements is/are correct? Viruses can infect - 1. Bacteria 2. Fungi 3. Plants Select the correct answer using the code given below: a) 1 and 2 only b) 3 only c) 1 and 3 only d) 1, 2 and 3 Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Answer: D A virus is a small infectious agent that replicates only inside the living cells of other organisms. Viruses can infect all types of life forms, from animals and plants to microorganisms, including bacteria and fungi. According to the type of the host they infect, viruses are classified mainly into the following four types: a. Plant viruses including algal viruses-RNA DNA b. Animal viruses including human viruses- DNA/RNA c. Fungal viruses(Mycoviruses)-ds RNA d. Bacterial viruses (Bacteriophages) including cyanophages-DNA Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Previous Year Questions: 2021 Q.Consider the following: 1. Bacteria 2. Fungi 3. Virus Which of the above can be cultured in an artificial/synthetic medium? a) 1 and 2 only b) 2 and 3 only c) 1 and 3 only d) 1, 2 and 3 Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Answer: A Bacteria and Fungi can be cultured in an artificial/synthetic medium. Whereas viruses require a living host cell for replication. Infected host cells (eukaryotic or prokaryotic) can be cultured and grown, and then the growth medium can be harvested as a source of the virus. Hence, 1 and 2 are correct and 3 is not correct. Therefore, option (a) is the correct answer Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Previous Year Questions: 2013 Q.Which of the following statements is/are correct? 1. Viruses lack enzymes necessary for the generation of energy. 2. Viruses can be cultured in any synthetic medium. 3. Viruses are transmitted from one organism to another by biological vectors only. Select the correct answer using the codes given below a) 1 only b) 1 and 2 only c) 1 and 3 only d) 1, 2 and 3 Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Answer: A Since Viruses lack organelles and are totally dependent on the host cells metabolic machinery for replication, (hence) they cannot be grown in synthetic media. In the laboratory, animal viruses are grown in animals or animal cell culture and plant viruses are grown in plants or plant cell culture. There are two categories of Vectors: biological and mechanical. As was stated earlier, if the virus increases its number while in association with a vector, then that vector is termed as being biological. Conversely, the vector is termed to be mechanical, if the virus doesnʼt increase its number while in association with that vector. Arthropods such as wasps, which repeatedly can sting multiple animals, could serve as mechanical vectors by transporting viruses on the surface of their stingers. Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Previous Year Questions: 2021 Q. Consider the following statements: 1. Adenoviruses have single-stranded DNA genomes whereas retroviruses have double-stranded DNA genomes. 2. Common cold is sometimes caused by an adenovirus whereas AIDS is caused by a retrovirus. Which of the statements given above is/are correct? a) 1 only b) 2 only c) Both 1 and 2 d) Neither 1 nor 2 Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Answer: B Adenovirus is a type of virus that has no envelope whereas retroviruses are characterized as enveloped viruses. Adenoviruses have double-stranded linear DNA and are associated with two major core proteins. A retrovirus is a virus that uses RNA as its genetic material. When a retrovirus infects a cell, it makes a DNA copy of its genome that is inserted into the DNA of the host cell. Hence, statement 1 is not correct. Adenoviruses are common viruses that cause a range of illnesses. They can cause cold-like symptoms, fever, sore throat, bronchitis, pneumonia, diarrhoea, and pink eye (conjunctivitis). Whereas, retroviruses can cause several human diseases such as some forms of cancer and AIDS. Hence, statement 2 is correct. Therefore, option (b) is the correct answer. Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Previous Year Questions: 2022 Q. Consider the following statements: DNA Barcoding can be a tool to: 1. assess the age of a plant or animal. 2. distinguish among species that look alike. 3. Identify undesirable animal or plant materials in processed foods. Which of the statements given above is/are correct? (a) 1 only (b) 3 only (c) 1 and 2 (d) 2 and 3 Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Answer: D DNA barcoding is a system for species identification focused on the use of a short, standardized genetic region acting as a “barcode” DNA barcoding has got no association with determining the age of an organism. DNA barcoding is a system for fast and accurate species identification that makes ecological system more accessible by using short DNA sequences instead of whole genome and is used for eukaryotes. In recent times, DNA barcoding has emerged as the most potent tool for detection of adulteration of food with unwanted plant and animal material. Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More Science & Tech Module Science and Technology by Dr. Shivin Chaudhary Click Here to Learn More