Biology 232 Microbiology Lab Exam 2 Study Guide PDF
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This document is a study guide for exam 2 in a microbiology laboratory course, titled Biology 232. It contains a list of questions covering various topics such as staining, Gram and acid-fast techniques, endospore staining, motility, and culturing. It references techniques like differential and negative staining, endospore methods like sporulation and germination, and discussions on motility.
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BIOLOGY 232 (Microbiology Laboratory) Study Guide Examination 2 1. Technique used for pure culture isolation? 2. Explain the purpose of staining microorganisms. 3. Differentiate between acidic and basic dyes. 4. Explain why basic dye...
BIOLOGY 232 (Microbiology Laboratory) Study Guide Examination 2 1. Technique used for pure culture isolation? 2. Explain the purpose of staining microorganisms. 3. Differentiate between acidic and basic dyes. 4. Explain why basic dyes are more effective in staining bacteria than acidic dyes 5. Explain why negative staining involves acidic dyes. List two acidic dyes 6. Know the basic steps involved in preparing a smear 7. List the reasons for heat fixing the smear 8. Explain the difference in preparing a smear from a broth culture and a culture on solid media 9. List the differential staining procedures you performed 10. List the Gram stain reagents: a. Primary dye b. Secondary dye c. Mordant d. Decolorizing agent 11. Explain the function of each reagent used in Gram staining 12. Significance of peptidoglycan in Gram reaction 13. Differentiate between Gram positive and Gram negative bacteria 14. Know the Gram staining procedure in detail. 15. What is the difference in cell wall (peptidoglycan) structure between Gram positive and Gram negative bacteria 16. Explain the medical importance of acid-fast staining procedure 17. List the pathogenic species of Mycobacteria 18. Name the bacterial genus that is acid-fast 19. List the reagents used for acid-fast staining: a. Primary dye b. Decolorizer c. secondary dye 20. Explain why heat is used in acid-fast staining 21. What is the difference in color between acid-fast and nonacid fast bacteria after performing the Ziehl-Neelsen procedure 22. List the bacterial genera that produce endospores 23. Function of endospore? 24. Locations of endospores within bacterial cells 25. Name the staining method used for endospore staining 26. Define:Sporulation, Germination 27. List the reagents used for spore staining: a. Primary dye b. Decolorizer c. Counter stain 28. Explain why heat is applied for endospore staining 29. function of flagella. Methods used for motility determination 30. what is the use of agar in culture media preparation? Describe the steps involved in culture media preparation 31. Differentiate between selective media and differential media; complex and defined medium 32. Differentiate: autotrophs and heterotrophs, chemoorganotrophs, chemolithotrophs 33. describe autoclaving
