Lab Exercise 4: Drug Admin & Blood Collection in Chickens PDF

Group No. ___ Date:___________ Group Members: Exercise IV: Drug Administration and Blood Collection in Chicken Introduction Objectives (at least 3): Materials Appropriate number of chicken per group 1cc and 3cc syringe Alcohol swabs...

Group No. ___ Date:___________ Group Members: Exercise IV: Drug Administration and Blood Collection in Chicken Introduction Objectives (at least 3): Materials Appropriate number of chicken per group 1cc and 3cc syringe Alcohol swabs Cotton swabs Trash bag & disinfectant Procedure Note: Each member is required to conduct drug administration (via IM/SC) and blood collection (via wing vein/jugular vein). Blood collection via brachial vein (wing vein) 1. Ask an assistant to hold the chicken horizontally on its back. The assistant uses one hand to hold the legs and places the other hand under the back to support the chicken. 2. Pull a wing of the chicken out towards you. 3. Note the wing vein, clearly visible running between the biceps and the triceps muscles. The wing vein forms a V (bifurcates). Note the tendon of the pronator muscle that runs across the V. 4. Pluck away any small feathers that obscure the vein. 5. Disinfect the area around the bleeding site by swabbing with 70 percent alcohol. 6. Insert the needle under the tendon. Direct the needle into the wing vein in the direction of the flow of blood. Do not insert the needle too deeply. Keep clear of the ulnar nerve. 7. Once the tip of the needle is in the vein, gently pull the plunger of the syringe. Blood will flow into the syringe. If blood does not flow, release the plunger and make a very slight adjustment to reposition the end of the needle. 8. Be patient and use a gentle suction to withdraw the blood. Chicken veins collapse readily. 9. If a haematoma forms, try bleeding from the other wing. 10. After removing the needle, apply pressure to the vein for a few seconds to discourage further bleeding. 11. Ideally the needle should be removed into a needle disposal container and the cap place on the end of the syringe to prevent leakage of the serum. However in many places these containers are not available and the cap will be placed over the needle. 12. Pull the plunger back approximately 1 cm and place the syringe at an angle with the needle end up in a rack facilitate clotting. Figure 1. Brachial vein blood collection Blood Collection via jugular vein 1. Place the bird on a table, setting it on its side. 2. Stretch out the neck with one hand and part the feathers along the neck. The right jugular vein is usually larger. 3. Place the needle at a slight angle, bevel up, against the vein. 4. Puncture the vein and slowly withdraw blood. 5. Remove the needle and apply pressure to the vein for a few seconds. 6. Fill the appropriate vial 1/3 to 1/2 of its full volume. Drug administration 1. Identify and make a table showing the different routes of drug administration in poultry, briefly discuss the procedure, and give examples of drug/vaccine for each route. (5pts) Table 1. Marking rubrics for blood collection Criteria Excellent Very Good Good Needs Poor (5pts) (4pts) (3pts) Improvement (1pt) (2pts) Blood collection (one-hit only) Blood collection (0.5ml) References 1. Morishita T.Y. (2019). Poultry Blood Collection. Western University of Health Sciences. Available at: https://www.westernu.edu/media/veterinary/poultry- health/2019-BloodCollection.pdf 2. FAO (n.d.) Collection of blood from chickens. FAO. Available at: https://www.fao.org/4/ac802e/ac802e0a.htm