INF 309 Practical Exam Outline (Jan 2025) PDF
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Uploaded by HalcyonFaith8570
Cairo University Medicine
2025
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Summary
This document provides an outline of a practical microbiology exam, INF 309, covering various topics like bacterial culture media, blood agar, antibiotic sensitivity, serology, and Gram staining. The exam is expected to be held in January 2025. It is a great resource for students preparing for the practical examination.
Full Transcript
INF 309 – Practical Exam stations Total 16 stations: 8 slides 5 culture media 1 BR 1 serology 1 antibiotic sensitivity INF 309 – slides (8 slides) 1. Staphylococci in culture 12. Clostridium tetani 2. Staphylococci in pus 13.Mycobacterium tuberculosis 3. Streptoc...
INF 309 – Practical Exam stations Total 16 stations: 8 slides 5 culture media 1 BR 1 serology 1 antibiotic sensitivity INF 309 – slides (8 slides) 1. Staphylococci in culture 12. Clostridium tetani 2. Staphylococci in pus 13.Mycobacterium tuberculosis 3. Streptococci in culture (Acid fast bacilli / Z-N stain) 4. Streptococci in pus 14.Mycobacterium leprae 5. Pneumococci in tissue (Acid Fast bacilli / modified Z-N stain) 6. Neisseria in culture 15.Gram negative bacilli 7. Pathogenic Neisseria in pus 16.Klebsiella in tissue 8. Corynebacterium diphtheriae 17.Gram negative bacilli & staphylococci (Gram stain) 18.Spirochaetes (Fontana stain) 9. Diphtheroids 19.Vincent’s angina 10.Bacillus anthracis 20.Candida (Gram stain) 21.Candida & Gram –ve bacilli 11.Bacillus anthracis (Spore stain) INF 309 – culture media (5) 1. Staphylococcus aureus on nutrient agar 10. Chocolate agar for Neisseria and Haemophilus 2. Pseudomonas aeruginosa on nutrient 11.Loffler’s serum for C. diphtheriae agar 12.Lowenstein Jensen (L.J) medium for M. 3. Proteus on nutrient agar tuberculosis 4. Mannitol salt agar with staph aureus or 13.Lactose & Non-lactose fermenter on MacConkey’s CoNs medium 5. Alpha-haemolytic organism on blood 14.Vibrio cholerae on T.C.B.S. agar 15.Cooked meat medium 6. Beta-haemolytic organism on blood agar for anaerobic cultivation 7. Non-haemolytic organism on blood agar 16.Anaerobic Jar 8. Bacitracin test Sensitive or Resistant 17.Tissue culture bottle 9. Optochin test Sensitive or Resistant INF 309 – BR (1) 1. Triple sugar iron( TSI ) 2. Urease test 3. Coagulase Test 4. Oxidase test 5. Indole test 6. Citrate test 7. B.R. of E. coli 8. B.R. of Klebsiella 9. B.R. of Proteus 10. B.R. of Salmonella 11. B.R. of Shigella INF 309 – Serology (1) 1. Brucella tube agglutination test showing prozone phenomenon 2. Widal Test 3. Single radial immunodiffusion 4. Elek’s Test 5. ELISA plate INF 309 – Antibiotic sensitivity (1) 1. Antibiotic sensitivity test (Disc diffusion method) 2. Antibiotic sensitivity test (Tube dilution method for MIC determination) 3. Antibiotic sensitivity test (E-test for MIC determination)
