Podcast
Questions and Answers
Which culture medium is specifically used for the cultivation of Neisseria and Haemophilus?
Which culture medium is specifically used for the cultivation of Neisseria and Haemophilus?
- Blood agar
- MacConkey's agar
- Chocolate agar (correct)
- Nutrient agar
What is the primary purpose of the triple sugar iron (TSI) test?
What is the primary purpose of the triple sugar iron (TSI) test?
- To identify coagulase-positive staphylococci
- To test for antibiotic sensitivity
- To determine the fermentation abilities of an organism (correct)
- To measure the urease production of bacteria
Which bacterial species is identified using the Elek's Test?
Which bacterial species is identified using the Elek's Test?
- Staphylococcus aureus
- Pseudomonas aeruginosa
- Corynebacterium diphtheriae (correct)
- Escherichia coli
For which organism would you use Lowenstein Jensen (L.J) medium?
For which organism would you use Lowenstein Jensen (L.J) medium?
Which test determines if an organism is sensitive or resistant to bacitracin?
Which test determines if an organism is sensitive or resistant to bacitracin?
What is the significance of identifying antibiotic sensitivity testing results?
What is the significance of identifying antibiotic sensitivity testing results?
Which organism is primarily characterized by its production of acid-fast bacilli?
Which organism is primarily characterized by its production of acid-fast bacilli?
Which serology test is designed to show the prozone phenomenon?
Which serology test is designed to show the prozone phenomenon?
Flashcards
Tube Dilution Method
Tube Dilution Method
A technique used to determine the susceptibility of bacteria to antibiotics using a series of tubes containing different concentrations of the antibiotic.
Disc Diffusion Method
Disc Diffusion Method
A method used to determine the sensitivity of bacteria to a specific antibiotic by observing the growth of the bacteria around a disc impregnated with the antibiotic on a plate.
E-test
E-test
A rapid method for determining the sensitivity of bacteria to antibiotics using a strip impregnated with a gradient of antibiotic.
Nutrient Agar
Nutrient Agar
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Chocolate Agar
Chocolate Agar
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MacConkey Agar
MacConkey Agar
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Lowenstein Jensen Medium
Lowenstein Jensen Medium
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Urease Test
Urease Test
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Study Notes
INF 309 - Practical Exam Stations
- Total of 16 stations
- 8 slides stations
- 5 culture media stations
- 1 blood cultures (BR) station
- 1 serology station
- 1 antibiotic sensitivity station
INF 309 - Slides (8 Slides):
- Staphylococci in culture
- Staphylococci in pus
- Streptococci in culture
- Streptococci in pus
- Pneumococci in tissue
- Neisseria in culture
- Pathogenic Neisseria in pus
- Corynebacterium diphtheriae (Gram stain)
- Diphtheroids
- Bacillus anthracis (Gram stain)
- Bacillus anthracis (Spore stain)
- Clostridium tetani
- Mycobacterium tuberculosis (Acid fast bacilli / Z-N stain)
- Mycobacterium leprae (Acid Fast bacilli / modified Z-N stain)
- Gram negative bacilli
- Klebsiella in tissue
- Gram negative bacilli & staphylococci
- Spirochaetes (Fontana stain)
- Vincent's angina
- Candida
- Candida & Gram -ve bacilli
INF 309 - Culture Media (5):
- Staphylococcus aureus on nutrient agar
- Pseudomonas aeruginosa on nutrient agar
- Proteus on nutrient agar
- Mannitol salt agar with staph aureus or CoNs
- Alpha-haemolytic organism on blood agar
- Beta-haemolytic organism on blood agar
- Non-haemolytic organism on blood agar
- Bacitracin test Sensitive or Resistant
- Optochin test Sensitive or Resistant
- Chocolate agar for Neisseria and Haemophilus
- Loffler's serum for C. diphtheriae
- Lowenstein Jensen (L.J) medium for M. tuberculosis
- Lactose & Non-lactose fermenter on MacConkey's medium
- Vibrio cholerae on T.C.B.S.
- Cooked meat medium for anaerobic cultivation
- Anaerobic Jar
- Tissue culture bottle
INF 309 - BR (1):
- Triple sugar iron (TSI)
- Urease test
- Coagulase Test
- Oxidase test
- Indole test
- Citrate test
- B.R. of E. coli
- B.R. of Klebsiella
- B.R. of Proteus
- B.R. of Salmonella
- B.R. of Shigella
INF 309 - Serology (1):
- Brucella tube agglutination test showing prozone phenomenon
- Widal Test
- Single radial immunodiffusion
- Elek's Test
- ELISA plate
INF 309 - Antibiotic Sensitivity (1):
- Antibiotic sensitivity test (Disc diffusion method)
- Antibiotic sensitivity test (Tube dilution method for MIC determination)
- Antibiotic sensitivity test (E-test for MIC determination)
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Description
This quiz covers the practical exam stations for INF 309, including a total of 16 stations focusing on slides, culture media, blood cultures, serology, and antibiotic sensitivity. Review key topics such as various bacterial cultures and identification techniques to prepare for your practical assessment.
