Haemetology Testing Methods 2024 PDF

MBS 332 HAEMATOLOGY TESTING METHODS MR H HARRIBHAI 2024  Basic Laboratory Techniques Phlebotomy: Blood Collection The first step in blood assessment is phlebotomy, where a healthcare professional collects blood from a patient, typically from a vein in the arm....

MBS 332 HAEMATOLOGY TESTING METHODS MR H HARRIBHAI 2024  Basic Laboratory Techniques Phlebotomy: Blood Collection The first step in blood assessment is phlebotomy, where a healthcare professional collects blood from a patient, typically from a vein in the arm. This process requires sterile techniques to prevent contamination and ensure accurate results. https://www.perplexity.ai/search/what-are-the-basic-laboratory-  Centrifugation Separation of Components: After collection, blood samples are often centrifuged to separate the components of blood into plasma, red blood cells, white blood cells, and platelets. Centrifugation uses high-speed rotation to create a gradient, allowing the different components to settle based on their density. https://www.perplexity.ai/search/what-are-the-basic-laboratory-  Complete Blood Count (CBC)Hematological Analysis: A CBC is one of the most common tests performed on whole blood. It measures various parameters, including:Red blood cell (RBC) count White blood cell (WBC) count Hemoglobin concentration Hematocrit (the proportion of blood volume occupied by red blood cells) Platelet count This test helps diagnose conditions like anemia, infections, and blood disorders by providing a snapshot of the blood's cellular composition https://www.perplexity.ai/search/what-are-the-basic-laboratory-i7Im5rD8QAWE7.yhBJVmIQ  Component Male Female White Blood Cells 4,500 to 11,000 cells/mcL 4,500 to 11,000 cells/mcL Red Blood Cells 4.5 to 5.6 million cells/mcL 3.8 to 5.2 million cells/mcL Hemoglobin 13.5 to 17.5 g/dL 12.0 to 15.5 g/dL Hematocrit 38.8% to 50% 36% to 48% Platelets 135,000 to 450,000/mcL 135,000 to 317,000/mcL https://drlogy.com/test/complete-blood-count-cbc  Biochemical Analysis Serum or Plasma Testing: Following centrifugation, the liquid portion (serum or plasma) can be analyzed for various biochemical markers, including: Electrolytes (sodium, potassium, calcium) Glucose levels Hormones Proteins These tests are crucial for assessing metabolic functions and identifying conditions such as diabetes or kidney disease https://www.perplexity.ai/search/what-are-the-basic-laboratory-i7Im5rD8QAWE7.yhBJVmIQ ps://www.labtestpk.com/plasma-and-serum-difference/  Coagulation Tests Clotting Function Assessment: Coagulation tests evaluate the blood's ability to clot. Common tests include: Prothrombin time (PT) Activated partial thromboplastin time (aPTT) International normalized ratio (INR) These tests are vital for diagnosing bleeding disorders and monitoring patients on anticoagulant therapy tps://www.lecturio.com/concepts/coagulation-studies/ https://www.slideserve.com/wells/coagulation-tests-powerpoint-ppt-  Immunoassays Antibody and Antigen Detection: Immunoassays are used to detect specific proteins, antibodies, or antigens in the blood. Techniques such as enzyme-linked immunosorbent assay (ELISA) and Western blotting are commonly employed to diagnose infections (e.g., HIV, Lyme disease) or autoimmune conditions For more information visit: https://www.news-medical.net/ life-sciences/What-are- Immunoassays.aspx  Flow Cytometry Cell Analysis Flow cytometry is a powerful technique that analyzes the physical and chemical characteristics of blood cells. It is particularly useful for diagnosing hematological malignancies like leukemia and lymphoma by identifying and counting specific cell types Flow cytometry is a standard laser-based technology that is used in the detection and measurement of physical and chemical characteristics of cells or particles in a heterogeneous fluid mixture. (https://microbenotes.com/flow-cytometry/) For more details on flow cytometry visit: (https://microbenotes.com/flow-cytometry/) ttps://microbenotes.com/flow-cytometry/