Glucose Identification Test PDF
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Menoufia National University
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Summary
This document details a series of chemical tests performed on an unknown solution to identify it as glucose. The tests, including Biuret, Molish, Iodine, Benedict, Fehling, Barfoed, Ketose, and Seliwanoff, provide specific observations and results that confirm the presence of reducing sugars and monosaccharides.
Full Transcript
## Physical properties: * **Volume:** random sample * **Aspect:** clear * **Reaction:** neutral * **Taste:** sweety * **Odour:** odourless * **Colour:** colourless | Test | Observation | Result | |---|---|---| | 1. Biuret test | Blue colour | Carbohydrate | | 2. Molish test | Violet ring between 2...
## Physical properties: * **Volume:** random sample * **Aspect:** clear * **Reaction:** neutral * **Taste:** sweety * **Odour:** odourless * **Colour:** colourless | Test | Observation | Result | |---|---|---| | 1. Biuret test | Blue colour | Carbohydrate | | 2. Molish test | Violet ring between 2 solutions | Carbohydrate | | 3. Iodine test | No change of yellow colour of iodine | Simple sugar | | 4. Benedict test | Red ppt | Reducing sugar (all mono and disaccharides except sucrose) | | 5. Fehling test | Red ppt | Reducing sugar (all mono and disaccharides except sucrose) | | 6. Barfoid test | Red mirror ppt before 5 minute | Monosaccharide (Glucose or fructose) | | 7. Ketose test | No Deep red colour | Sugar does not contain ketone group (glucose) | | 8. Seliwanoff test | No Cherry red colour | Sugar does not contain ketone group (glucose) | **Name of Unknown solution** Glucose
