Citric Acid Cycle Part 1a Pre-learning 3: General Biochem 4 PDF

Citric Acid Cycle Part 1a Prelearning: Intro to CAC with connections to beta oxidation and glycolysis Dr. Heisel BMS100 Citric Acid Cyle CAC = Kreb’s cycle, TCA (tricarboxylic acid cycle) Energy producing pathway found in mitochondria Indirect: NADH, FADH2 Direct: ATP (GTP) Final common pathway for...

Citric Acid Cycle Part 1a Prelearning: Intro to CAC with connections to beta oxidation and glycolysis Dr. Heisel BMS100 Citric Acid Cyle CAC = Kreb’s cycle, TCA (tricarboxylic acid cycle) Energy producing pathway found in mitochondria Indirect: NADH, FADH2 Direct: ATP (GTP) Final common pathway for catabolism/oxidation of: Glucose (next slide) Fatty acids (next slide) Amino acids (next presentation) CAC Glucose Fatty acid Glycolysis NADH, ATP Pyruvate Fatty acyl Pyruvate Β-ox NADH, FADH2 Acetyl CoA CAC NADH, FADH2, ATP NADH Citric Acid Cycle Creation of acetyl CoA after glycolysis Rxn occurs in the mitochondrial matrix Exergonic and irreversible Enzyme = pyruvate dehydrogenase complex C=O CH3 Citric Acid Cycle Once you have acetyl CoA from glycolysis or beta oxidation, how does it enter CAC? Glucose Fatty acids Pyruvate Acetyl CoA Oxaloacetate Fumarate CAC Succinyl CoA Citrate α-ketoglutarate 5 Citric Acid Cycle Acetyl CoA and oxaloacetate combine to make citrate Enzyme: Citrate synthase CoA helps transfer the acetyl group to oxaloacetate Citrate is the first molecule of CAC Citrate Synthase HO- =O Check your knowledge Both glycolysis and beta ox ultimately connect to CAC via what molecule? What preparatory step (substrate(s), enzyme and product(s)) is needed to connect glycolysis to CAC? Does it make or use indirect energy? What is the first reaction of CAC (substrate(s), enzyme, product(s))?