General Biochem 4 (Pre-learning 4) PDF

Citric Acid Cycle Part 1b Prelearning: Amino acid connections to CAC Dr. Heisel BMS100 Citric Acid Cycle: Entry points Review: Glycolysis and beta oxidation connect to CAC via acetyl CoA How do amino acids feed into CAC? Glucose Ala*, Cys, Gly, Ser, Thr, Trp Fatty acids Pyruvate Acetyl CoA Oxaloacet...

Citric Acid Cycle Part 1b Prelearning: Amino acid connections to CAC Dr. Heisel BMS100 Citric Acid Cycle: Entry points Review: Glycolysis and beta oxidation connect to CAC via acetyl CoA How do amino acids feed into CAC? Glucose Ala*, Cys, Gly, Ser, Thr, Trp Fatty acids Pyruvate Acetyl CoA Oxaloacetate Leu, Phe, Tyr, Trp, Lys, Thr, Ile Asp*, Asn Fumarate CAC Asp*, Phe, Tyr Succinyl CoA Ile, Met, Val Citrate α-ketoglutarate Arg, Gln, His, Pro, Glu* 2 Citric Acid Cycle: Amino Acids Amino acid entry points to know for now: Aspartate to oxaloacetate Glutamate to alpha ketoglutarate Alanine to pyruvate All follow same pattern: the amino group on the amino acid becomes a double-bonded O Also know: Aspartate to fumarate This conversion will also be important in the urea cycle Citric Acid Cycle: Amino Acids Alanine Aspartate Glutamate O = O= = O Pyruvate Oxaloacetate α-Ketoglutarate Citric Acid Cycle Overall reaction Acetyl-CoA + 3NAD+ + FAD + GDP + Pi + 2H2O Ú 2CO2 + 3 H+ + 3NADH + FADH2 + GTP + CoASH Waste Reduced intermediates: Direct Regenerated Indirect energy energy coenzyme (converts to ATP) GTP + ADP ∆Go’ = 0.0 kJ/mol GDP + ATP Citric Acid Cycle 3 1 1 1 2 1 2 Check your knowledge Catabolism of what 3 types of molecules can feed into CAC? Name one amino acid that can connect to CAC via: Pyruvate alpha-ketoglutarate Fumarate Oxaloacetate What is the overall energy yield from 1 round of CAC? (see beta ox prelearning 1b for answer if needed)