Bacterial Classification PDF
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Liceo de Cagayan University
Coleen M. Rante,R.M.T
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Summary
This document provides a summary of bacteria, listing characteristics, diseases, and diagnostic tests for diverse groups of bacteria. Some bacteria are gram positive cocci, while others are gram-negative bacilli. The information includes virulence factors, toxins, and other relevant details for each bacterial group.
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CATALASE POSITIVE, GRAM- POSITIVE COCCI Organism General Characteristics Diseases Diagnostic Tests Micrococcacea Catalase + Staphylococcus...
CATALASE POSITIVE, GRAM- POSITIVE COCCI Organism General Characteristics Diseases Diagnostic Tests Micrococcacea Catalase + Staphylococcus Gram + cocci in Toxic shock syndrome Catalase + clusters with PMN Scalded skin syndrome Coagulase + cells (Ritter’s disease) B-hemolytic Grape-like clusters Most common cause of PENICILLIN Staphyloxanthin septic arthritis in RESISTANT (yellow or golden on prepubertal children B-lactamase Staphylococcus aureus BA) Common cause of nosocomial pneumonia Acute endocarditis (IV drug user; tricuspid valve) Virulence Factors: Toxins: Polysaccharide capsule Alpha toxin Slime layer or biofilm Beta toxin Peptidoglycan Delta toxin Protein A Gamma toxin Coagulase Heat stable Hyaluronidase enterotoxin Lipase TSST-1 PVL Exfoliative toxin or epidermolytic toxin Gram + cocci in Most common cause of Catalase + clusters prosthetic valve Coagulase – endocarditis Staphylococcus epidermidis Small-medium, Implantation of NOVOBIOCIN opaque, gray white medical devices SENSITIVE colonies, slime- Non-hemolytic producing strains are extremely sticky Virulence Factors: Polysaccharide capsule Slime layer or biofilm Peptidoglycan Delta toxin Gram + cocci in Second most common Catalase + clusters cause of UTI in young Coagulase – Staphylococcus saprophyticus Large, entire very females NOVOBIOCIN glossy, smooth, RESISTANT opaque butyrous, Non-hemolytic covex Virulence Factor: Peptidoglycan Dog bite wound Misidentified as S. Staphylococcus intermedius infections aureus (Coagulase -) Staphylococcus lugdunensis Staphylococcus scheliferi Micrococcus Normal Flora Catalase + Coagulase – Modified oxidase test + BACITRACIN SENSITIVE FURAZOLIDONE RESISTANT Stomatococcus Normal Flora CATALASE NEGATIVE, GRAM- POSITIVE COCCI Streptococcus Catalase - Gram + cocci in chains Scarlet fever (SPEs) Catalase – Round to oval shaped Rheumatic fever (M B-hemolytic occasionally forming protein) BACITRACIN elongated cells Necrotizing fasciitis SENSITIVE resembles Honey-colored crusts PYR + Streptococcus pyogenes (Group pleomorphic (impetigo) A) corynebacterial or Most common lactobacilli bacterial cause Honey-colored crusts pharyngitis Virulence factors Streptlysin O Streptolysin A SPEs M protein Hyaluronidase C5a peptidase Erythrogenic toxin Streptokinase Protein f DNASeB Exotoxin B Capsule (most Pneumonia Catalase – important virulence) 35-37 days of gestation B-hemolytic Grayish-white mucoid tests for GBS BACITRACIN Streptococcus agalactiae colonies surrounded RESISTANT (Group B) PYR – by a small zone of CAMP + βhemolysis Hippurate hydrolysis + SXT R Virulence Factors Capsule (Important) DNASe, hyaluronidase Leukocytosis, lancet- Leading cause of Catalase – shaped in pairs, singly, bacterial meningitis in A-hemolytic Streptococcus pneumoniae or short chains infants, young children, Bile solubility S Round, glistening, wet, and adults ( N. (soluble) mucoid, dome shaped meningitidis, H. Optochin S appearance influenzae) Quellung + Virulence Factors Capsule Pneumolysin Phosphorylcholine Enterococcus (Group D) UTIs PYR + a. Enterococcus faecalis Bacteremia 6.5% NaCl growth Endocarditis LAP + b. Enterococcus faecium Cephalosporins, aminoglycosides R (VRE) Virulence Factors Aggregation substance Capsular polysaccharides Surface carbohydrates Ability to translocate across intact intestinal mucosa Hemolysis Lipoteichoic acid Gelatinase Superoxide production Peptide inhibitors Ability to adhere to extracellular matrix proteins Butterscotch smell on Dental caries (S. Viridans Streptococci CA mutans) Subacute bacterial endocarditis CATALASE POSITIVE, NON- BRANCHING, GRAM-POSITIVE BACILLI Bacillus Aerobic Catalase + Spore-forming rods Bacillus anthracis Large, in singles, pairs Cutaneous anthrax or serpentine chains Gastrointestinal Spore-forming (ingestion) anthrax BA: medium-large, Inhalation anthrax – gray, flat irregular Wool sorter’s disease swirling projections and ragpickers’ Box car-like rods disease “comet tails” or Injectional anthrax Medusa Head” or ground glass colonies Virulence Factors Lethal toxin Edema toxin Large, in singles, pairs Ingestion of fried rice BCYE or serpentine chains Food poisoning: Bacillus cereus diarrhea and abdominal pain Toxins Hemolysin BL Nonhemolytic enterotoxin Cytotoxin K Cereulide LISTERIA, CORYNEBACTERIUM, and Similar Organisms Non-spore forming Catalase – Short rod, occurs Listeriosis VP + Listeria monocytogenes singly or short chains Stillbirth CAMP + (resembles Esculin + streptococci) PCR End-over-end Elek Test tumbling motility Umbrella-shaped pattern Virulence Factors Lesteriolysin O Act A Corynebacterium Not normal microbiota Respiratory diphtheria Ribotyping, pulse-field Arranged singly in (pseudomebrane) gel electrophoresis, palisades of parallel, multilocus sequence or in pairs of cells typing Corynebacterium diptheriae forming V or L shapes Chinese letters Brown-black colonies with a gray-brown halo in TIN Improper care of IV Corynebacterium jeikeium catheters Urological Corynebacterium urealyticum manipulation Red coccus Rhodococcus CATALASE NEGATIVE, NON- BRANCHING, GRAM-POSITIVE BACILLI Short rods and long Cellulitis (erysipeloid) Catalase – Erysipelothrix spp filaments H2S + “bottle brush” Delicate, curved, rods Pharyngitis Arcanobacterium spp with pointed ends, occasional rudimentary branching T. bernerdiae: CAMP - Trueperella spp necrotizing fasciitis Highly pleomorphic, Maintaning pH balance A-hemolytic Lactobacillus spp long chaining rods in Dental caries coccobacilli and spiral forms Small, pleomorphic, Bacterial vaginosis B-hemolytic gray, opaque, gram- variable/gram –, Gardnerella vaginalis coccobacilli and short rods Clue cells Fishy odor BRANCHING OR PARTIALLY ACID-FAST, GRAM-POSITIVE BACILLI Nocardia Strictly aerobic Catalase + Beaded appearance Acid Fast (V) Pleomorphic Pulmonary Acid fast Dry and heaped (immunocompromised) Nocardia asteroides colonies, similar to fungus Soil or musty basement odor Actinomadura Mycetoma Not Acid fast Streptomyces GRAM-NEGATIVE BACILLI AND COCCOBACILLI Greenish metallic Most common cause of IMViC: ++ – – Escherichia coli sheen on EMB or MAC UTI in females Lactose + Pili Oxidase – Watery diarrhea, HE a. ETEC and HS Watery diarrhea (long) b. EPEC Bloody diarrhea, (Triad: c. EHEC hemorrhagic colitis Thrombocytopenia, HUS uremia, hemolytic anemia) Bloody diarrhea d. EIEC Persistent watery e. EAEC diarrhea (w/ HIV) Bacillary dysentery Lactose – Shigella Fever, watery then 100, 000 (high Salmonella Enterocolitis infective dose) H2S + Lysine + Indole – Urea – Proteus “burnt chocolate” or Urea + chocolate cake odor Deaminase + Motile Indole + Proteus vulgaris H2S + “swarming motility” Complicated UTI Indole – Proteus mirabilis (staghorn calculi) Urease + Contaminated medical Motile Enterobacter devices Lactose fermenter Mucoid colony Red pigment Motile Serratia (prodigiosin) Slow lactose fermenter: ONPG + DNAse, gelatinase + VP + Yersinia Rods with bipolar Bubonic plague Yersinia pestis (safety pin) staining Invasive, toxigenic Cold enrichment Yersinia enterocolitica Acute mesenteric Yersinia pseudotuberculosis lymphadenitis and “pseudotubercles” Environmental niche (water or soil) Plump coccobacilli, Non-lactose fermenter resist alcohol Purplish hue (mistaken decolorization for lactose fermenter) BA: Smooth, plaque, raised, creamy, and Acinetobacter spp. smaller than Enterobacteriaceae Water organism, Irrigating and IV solutions Short-medium straight RF: Cystic fibrosis Non-lactose fermenter rods BA: Large, smooth, Stenotrophomonas maltophilia glistening colonies with uneven edges and underneath growth Ammonia smell GRAM-NEGATIVE BACILLI AND COCCOBACILLI Burkholderia spp. Not normal microbiota BCSA Cystic fibrosis or CGD Burkholderia cepacian complex Coccobacillius Severe infections in Burkholderia mallei horses and related animals Bipolar staining Survives in human BA: cream to yellow- macrophages Burkholderia pseudomallei orange, smooth, Melioidosis mucoid MAC: pink BA: Dry and wrinkled Putrid odor Obligate aerobe Pneumonia: ventilator- Oxidase + Pseudomonas spp. Biofilm acquired pneumonia Pyocyanin, pyoverdin, BA: spreading, flat, Third most common cause pyorubrin, serrated edges, of nosocomial UTI pyomelanin on confluent growth, Associated with disrupted Mueller-Hinton often with metallic epidermis, cystic fibrosis sheen; bluish greed, Immunocompromised: red, or brown ecthyma gangrenosum, pigment diabetes, malignant otitis Sweet grape-like media odor or corn tortilla- like odor Pyocyanin (blue phenazine pigment) Virulence factors Exotoxin A Exoenzyme S and C Proteolytic enzymes (Elastases) Hemolysis (Phospholipase C) Pili and adhesins Alginate Pyocyanin catalases Alcaligenes Strict aerobic rods Bacteremia, ocular Catalase + Alcaligenes faecalis Medium-long rods infections, pancreatic Oxidase + BA: feathered edge abscess, bone infection, Motile (1-12 Alcaligenes aquatiis colonies surrounded urine and ear discharge peritrichous flagella) by zone of green Non-glucose utilizer discoloration Fruity odor resembling apples or strawberries Vibrio Straight or slightly Oxidase + curved rods Glucose fermenter Rapid or darting Cary-Blair Medium shooting-star motility Pandemic: V. cholera Cholera, bacteremia. B-hemolytic Vibrio cholera 01 biotype El Tor Septicemia (V. vulnificus), Rice-water stools gastroenteritis and would Vibrio parahaemolyticus: raw infection (V. seafood parahaemolyticus, V. vulnificus) Vibrio vulnificus: raw shellfish Virulence factors/Toxins Cholera toxin Toxin coregulated pili Zona occludens toxin V. cholera O1 V. cholera O139 Straight rods with Gastroenteritis in children Contain no to little Aeromonas spp. rounded ends or Severe watery diarrhea WBC coccobacilli HUS and kidney disease Modified CIN Slightly curved, Chromobacterium violaceum medium to long, with rounded ends Almond-like odor (ammonium cyanide) Violacei (ethaol soluble violet pigment) Facultative anaerobe From human bites or Assacharolytic Eikenella corrodens Slender, medium- clenched fist wounds Oxidase + length, with rounded Dental plaque Catalase – ends Subacute endocarditis Reduces nitrate to Pit or corrode the (HACEK) nitrite agar Hydrolyzes ornithine Chlorine bleach odor and lysine Pasteurella Oxidase + Ferment glucose Short, straight bacilli Respiratory disease BA: convex, smooth, Endocarditis and gray, nonhemolytic, septicemia (RF: liver P. multocida subsp. multocida rough and mucoid cirrhosis) Must or mushroom odor Short, plump Throat of children younger Ferment glucose Kingella kingae coccobacilli with than 4 years old squared-off ends in HACEK pairs or cluster, or form in chains BA: small, with B- hemolysis, may pit GRAM-NEGATIVE BACILLI AND COCCOBACILLI Haemophilus Small, pleomorphic, Non-motile coccobacillary or short rods Smooth, flat, or convex, buff, or slightly yellow colonies Requires protoporphyrin IX: X and V Factor, NAD or NADP Pleomorphic Haemophilus influenza coccobacilli or small rods Typable Nontypable (NTHi) Hib Not part of normal Chancroid, Sexual Haemophilus ducreyi microbiota contact “school of fish” Painful genital ulcer A. small, pleomorphic a. Haemophilus parainfluenza rods or long filamentous forms b. Haemophilus B. small coccobacilli or parahaemolyticus short rods with occasional cells appearing as tangled filaments Bartonella Facultative, Culture-negative Catalase – intracellular fastidious endocarditis Oxidase – organisms Urease – Short, pleomorphic, Nitrate reductase – rod-shaped Multiply and persist in RBC Acute hemolytic Bartonella bacilliformis bacteria (Oroya fever) or chronic vasoproliferative disease (chronic: Carrison disease) Trench fever Bartonella Quintana Bacillary angiomatosis Bacteremia, endocarditis, chronic lymphadenopathy; HIV patients Irregular, dry, white Focal suppurative Bartonella henselae colonies that pit agar reaction (cat scratch Small, circular, tan disease) moist colonies Multicocal “cauliflower-like” angioproliferative lesion (bacillary angiomatosis Bacteremia, endocarditis, rheumatic, bacillary peliosis hepatitis or splenic peliosis Afipia felis CD MICROAEROBIC (5-10% OXYGEN) ATMOSPHERE Campylobacter spp. Slow growing, Febrile systemic Asaccharolytic fastidious disease Catalase + Small, curved, or Periodontal disease Oxidase + seagull-winged, faintly Gastroenteritis Unable to grow 3.5% staining Extraintestinal NaCl Gray to pink or yellow infections gray and slightly Meningitis, mucoid endocarditis, Tailing effect along the septic arthritis streak lines (AIDS) Post: Reactive arthritis and Guillain-Barre Syndrome Acute imflammatory C. jejuni, (& C. coli) enteritis Arcobacter spp. Slow growing, Persistent watery Asaccharolytic fastidious diarrhea Arcobacter cryaerophilus Gastroenteritis Bacteremia, endocarditis, peritonitis Helicobacter spp. Curved, helical or Gastric ulcer Catalase + spiral, or fusiform Peptic ulcer disease Oxidase + Helicobacter pylori microaerophilic with or Gastric cancer Produce urease without periplasmic Atrophic gastritis Motile fibers Urea breath test + Small, translucent, circular colonies Legionella Mesophilic (20-45C) Multiply within amoeba Utilize protein Obligate aerobic Legionnaire’s disease Motile Faintly staining, thin, Pontiac fever fastidious Extrapulmonary Facultative intracellular Small, filamentous Brucella Small coccobacilli that Brucellosis Catalase + resemble fine grains of Urease + sand Most strains are Brucella abortus oxidase + Hydrolyzes urea Brucella melitensis Relative ability to produce H2S Brucella suis Titer of 1:160 Brucella canis Bordotella Minute, faintly staining Pertussis (whooping Direct fluorescent coccobacilli singly or in cough) antibody test Bordotella bronchiseptica pairs Whole cell Small and shiny, agglutination reactions Bordotella pertussis resembling mercury Titer greater than 100- drops 125 IU/mL Bordotella parapertussis Facultative, Human and animal Oxidase + Francisella intracellular organism tularemia Urease – Strict aerobes Rabbit fever, deer fly W Catalase + Transparent, mucoid, fever, market man’s Carbohydrate and easily emulsified disease fermenter “rabbits” Streptobacillus moniliformis Facultative, Nonaerobe Rat-bite fever Indole – Pleomorhic, long, Haverhill fever: Catalse – tangled chains and humans Oxidase – filaments with bulbar rash on the palms, Nitrate – swelling soles of the feet, and Nonmotile “fried egg” appearance other extremities Urea – with a dark center and Complications: Lysine decarboxylase a flattened lacy edge endocarditis, septic – L-phase arthritism, pneumonia, Broth: “fluff balls” or pericarditis, brain ”breadcrumbs” abscess, amnionitis, prostatitis, and pancreatitis Spirillum minus Gram-negative, helical, Rat-bite fever strictly aerobic Asia: sodoku organism Same with arthritis Thick, spiral, with 2 or Swollen lymph nodes 3 coils and polytrichous polar flagella Neisseria gonorrhea Diplococci with Oxidase + adjacent sides flattened Diplococci inside PMNs ”kidney- or coffee bean-shaped” Neisseria gonnorhoeae 2nd most commonly reported STI Sexually transmitted Neisseria meningitides Respiratory droplets Neisseria sicca and Neisseria Infectious endocarditis lactamica or bacteremia Leading cause of fatal Oxidase + bacterial meningitis in Reduces nitrate to children and adults nitrite Young children: otitis Unable to utilize media carbohydrates Moraxella catarrhalis Older adults: chronic Production of DNAse upper respiratory tract infections ANAEROBIC BACTERIOLOGY Strict anaerobes (0% oxygen) Aerotolerant organisms (5% oxygen) Foul odor Sulfur granules Brick red fluorescence under long wavelength UV light GRAM-POSITIVE, SPORE FORMING BACILLI, ANAEROBIC Clostridia Obligately anaerobe Catalase - (or aerotolerant) Produces yellow, Antibiotic-associated Clostridia difficile ground glass colonies diarrhea and on CCFA pseudomembranous chartreuse enterocolitis fluorescence upon exposure to UV light Horse stable odor Botulism Clostridia botulinum Infant botulinum (Floppy Baby Syndrome) Rods with round, Tetanus (lockjaw) Clostridia tetani terminal spores Drumstick or tennis racket appearance C. perfringens: double Clostridia perfringes, Clostridia zone hemolysis on septicum, Clostridia anerobic BA and sporogenes, Clostridia novyi reverse CAMP test positive C. septicum: medusa head appearance Fresh liquid or Suspected C. difficile infection unformed stools (CDI) should be processed GRAM-POSITIVE, NON-SPORE FORMING BACILLI Eggerthella and Periodontal disease Actinomyces Paraeggerthella Gram-positive filaments when crushed “sulfur granules” Gram-variable, curved Bacterial Vaginosis Mobiluncus rods with tapered ends Rod-shaped facultative Actinobaculum spp. anaerobes P. acnes Propionibacterium spp. Anaerobic diphtherioids Pleomorphic Strictly anaerobic or Bifidobacterium spp microaerophilic Rods or are branched or club-shaped Catalase – Lactobacillus spp. Lactic acid from glucose fermentation Eubacterium Normal intestinal and Nitrate + oral flora Catalase – E. lentum: Eggerthella and Intraabdominal and Paraeggerthella periabdominal infections GRAM-NEGATIVE RODS Mucosal surfaces of Saccharolytic Bacteroides fragilis Group the human oral cavity BILE RESISTANT and GIT Nonpigmented Dental biofilms BILE SENSITIVE Nonpigmented Prevotella spp. KANAMYCIN RESISTANT Brick red or produce Asaccharolytic and Pigmented Porphyromonas and brown to black pigmented Prevotella spp pigment Large, grey, and KANAMYCIN Fusobacteriaceae convoluted SENSITIVE Most strains fluoresce chartreuse Resembles D. orale: periodontal Asaccharolytic Proteobacteria Campylobacter disease BILE RESISTANT ureolyticus Desulfomicrobium spp.: motile, spiral- shaped organisms capable of reducing sulfate ANAEROBIC GRAM-POSITVE AND GRAM-NEGATIVE COCCI Gram-negative anaerobic cocci Peptrostreptoccocus Gram-positive cocci: anaerobius and VANCOMYCIN SENSITIVE Veillonela Parvimonas micra: S to COLISTIN RESISTANT sodium polyanethol Gram-negative cocci sulfate (SPS) VANCOMYCIN RESISTANT P. micra: milky halo around the colonies on BA MYCOBACTERIUM Mycobacterium tuberculosis Primary TB Tuberculin skin test or complex Meningeal or military purified protein TB Latent TB derivative test (PPD) Reactivation TB test T-Spot TB test Niacin Test + GIT mucosa or invade Mycobacterium bovis the lymphatic tissue of the oropharynx between M. Mycobacterium africanum tuberculosis and M. bovis 31% of the cases of PYRAZINAMIDE Mycobacterium caprae human TB SENSITIVE TB in both Mycobacterium microti immunocompetent and immunosuppressed patients lymphadenitis and Mycobacterium canetti generalized tuberculosis in immunocompromised individuals granulomatous lesions Mycobacterium pinnipedii in the lymph nodes, lungs, pleura, and spleen banded mongoose Mycobacterium mungi large mammals Mycobacterium orygis (gazelle, antelopes, and oryxes) Nontuberculous Mycobacteria a. Photochromogens Mycobacterium kansasii b. Scotochromogens c. Nonphotochromogens Mycobacterium terrae complex (M. terrae, M. triviale, M. nonchromogenicum) Mycobacterium gastri Mycobacterium avium complex Opaque, glossy, white, Respiratory disease (MAC) colony morphology or clinically similar to TB produce smaller in adults, translucent colony lymphadenitis in morphology children, and disseminated infection in patients with HIV d. Rapid growers Weakly gram-positive medical interventions rods resembling (BM transplantation), Mycobacterium abscessus sbsp. diphtheriods wound infections, and abscessus, Mycobacterium catheter sepsis chelonae, Mycobacterium fortuitum NONCULTIVATABLE NONTUBERCULOUS MYCOBACTERIA slender, poorly Leprosy (Hansen Footpads of armadillo Mycobacterium leprae stained, beaded, gram- disease) and footpads of mice positive bacilli Tuberculoid leprosy: “gram neutral,” or localized form “gram ghosts,” Lepromatous leprosy: ZN: appear red or have disseminated form a red or blue, beaded appearance Niacin M. tuberculosis Nitrate Reduction M. tuberculosis, M. kansasii, M. szulgai, and M. fortuitum Catalase + except f M. tuberculosis complex (some isoniazid-resistant strains) and M. gastri Tween 80 Hydrolysis nonpathogenic, slow-growing scotochromogens and nonphotochromogens Tellurite reduction MAC (3-4 dys) Arylsulfatase 3-day test: rapid growers M. fortuitum and M. chelonae 14-day test: M. marinum and M. szulgai Growth Inhibition by Thiophene-2- only M. bovis is unable to grow Carboxylic Acid Hydrazide OBLIGATE INTRACELLULAR AND NONCULTURABLE BACTERIAL AGENTS Chlamydia Most common sexually Glycogen: Yes Chlamydia trachomatis transmitted bacterial Plasmid: Yes pathogen and a major Sulfonamides S: Yes cause of pelvic inflammatory disease (PID), ectopic pregnancy, and infertility Trachoma Lymphogranuloma Venereum Oculogenital Infections Endemic pathogen of Glycogen: No Chlamydia psittaci all bird species Plasmid: Yes Sulfonamides S: No Single IgM titer of 1:32 Pear-shaped EB Pneumonia, bronchitis, Glycogen: No Chlamydia pneumoniae pharyngitis, sinusitis, Plasmid: No and a flu-like illness Sulfonamides S: No RICKETTSIA, ORIENTA, ANAPLASMA, and EHRLICHIA Rickettsia Fastidious bacteria Triad of fever, Obligate, intracellular headache, and rash parasites Ticks Mediterranean and R. conorii Israeli spotted fecers Ticks Rocky mountain R. ricketsii spotted fever Mild R. parkeri Ticks Lice Epidemic typhus R. prowazekii Fleas Murine typhus R. typhi Chiggers Scrub typhus Orienta tsutsugamushi Human monocytic Ehrlichia chaffeensis ehrlichiosis (HME) Human granulocytic Anaplasma phagocytophilum anaplasmosis (HGA) Q fever Coxiella Whipple disease Trophryma whipplei Encapsulated, Granuloma inguinale Klebsiella granulomatis pleomorphic, gram- or donovanosis negative bacillus form beefy, Donovan body: groups erythematous, of organisms seen granulomatous, within mononuclear painless lesions endothelial cells that bleed easily Safety pin appearance: organism stains as a blue rod with prominent polar granules surrounded by a large, pink capsule CELL WALL-DEFICIENT BACTERIA Mycoplasmas M. pirum, M. fermentans, and M. penetrans: isolated from patients infected with HIV M. genitalium: 15% to 20% of nongonococcal urethritis; females: associated with cervicitis and pelvic inflammatory disease (PID) spherical, grainy, Mycoplasma pneumoniae yellowish forms that are embedded in the agar, with a thin outer layer “fried egg” appearance Urease – Mycoplasma hominis Ureaplasma urealyticum A8 agar; urease Pregnancy-related production with complications; calcium chloride commonly colonizes indicator; infants colonies appear as Ureaplasma and dark brownish Mycoplasma spp.: clumps isolated from the internal organs of stillborn, premature, and spontaneously aborted fetuses SPIROCHETES axial fibrils and an outer sheath slender with tight coils Venereal syphilis Treponema Primary Syphilis: chancre Secondary Syphilis”: “moth- eaten” area Tertiary syphilis: neurosyphilis and gummas Congenital syphilis “saber shin” bowing of the tibia “bull dog” appearance of a deformed maxilla somewhat thicker with Borreliosis: relapsing S+ Hippurate Borrelia, B. burgdorferi fewer and looser coils fever hydrolysis Lyme disease W indole resemble Borrelia Leptospirosis Leptospira except for their hooked ends spinning motility or a rapid back-and-forth movement comma-shaped or Brachyspira helical, with tapered ends with four flagella at each end “false brush border”