Bacteriology Lab (Finals) PDF

Bacteriology Lab (Finals) Dilution - created by measuring a volume of a sample Lesson 1: Bacterial Transfer and adding it to a volume of sterile water,...

Bacteriology Lab (Finals) Dilution - created by measuring a volume of a sample Lesson 1: Bacterial Transfer and adding it to a volume of sterile water, thus making the resulting solution less 1. Streak Plate Technique concentrated than the original. - is essentially a dilution technique that is done by streaking a loopful of supposedly Serial Dilution mixed culture several times over the surface - repetitive process of dilution for each new of an agar plate. tube, thus making a series of more and more - uses a L-shaped rod dilute samples. SD=_________Amount Transfered_________ New Total Volume (Amount Transferred + Volume of Diluent) Serial Dilution Procedure Numbers of organisms can be graded: 1. Make a Serial Dilution of tubes using a 1ml in 9ml dilution. Ex: B = Limited, or 1+ C = Moderate, or 2+ D = Heavy, or 3+ to 4+ SD =_________Amount Transferred________ New Total Volume (Amount Transferred + Volume of Diluent) = ___1ml__ 1ml + 9ml = _1ml_ 10ml = 1:10 or 1. 2. Spread Plate Method 10 2. Determine the dilution of each tube. 3. Pour Plate Method Total Dilution = Previous x Current Dilution Dilution = 1 x 1. 10 10 = 1 or 1. 100 3. Transfer 0.1ml of the sample onto plates and Other Composition: count the colonies of each using the Colony Counter. Determine which are TNTC and TFTC. Phenol red - pH indicator Ferrous sulfate - indicator for H2S production Other Test Used: Kligler Iron Agar (KIA) - also differential - composed of glucose and lactose only TSI Results >300 Too Numerous/Many To Count (TNTC/TMTC) Fermenter - can make agar environment Acid