Aseptic Technique PDF Past Paper

Aseptic Technique Answer the questions below. 1. Describe the difference between eukaryotic and prokaryotic cells. 2. Describe the function of mitochondria in eukaryotic cells. Do Now 3. Give an example of a eukaryotic and a pr...

Aseptic Technique Answer the questions below. 1. Describe the difference between eukaryotic and prokaryotic cells. 2. Describe the function of mitochondria in eukaryotic cells. Do Now 3. Give an example of a eukaryotic and a prokaryotic cell. 4. Describe the difference between unicellular and multicellular organisms. 5. State an example of a unicellular organism.  22/08/2024 Aseptic Technique B3.1.3 B3.1.1 Prior Knowledge Review B3.1.8 Diffusion in Living Things B3.1.2 Eukaryotic and Prokaryotic Cells B3.1.9 Osmosis B3.1.3 Aseptic Technique B3.1.10 Osmosis Investigation B3.1.4 Growth of Bacteria B3.1.11 Active Transport B3.1.5 Microscopes B3.1.12 Cell Division B3.1.6 Observing Cells B3.1.13 Cancer B3.1.7 Diffusion B3.1.14 Stem Cells Following this lesson, students will be able to: State the stages of aseptic technique when growing bacteria Lesson Objectives Describe the function of the agar medium Explain why the Petri dish is not secured with an airtight seal when growing bacteria Key Words: aseptic antibiotic culture agar medium inoculating loop sterilisation General Definition Scientific Definition Noun: The growing of microorganisms (such as bacteria) The ideas, customs and social for scientific study. behaviours of a group or society. The arts From the Latin cultura Vocabulary Culture ‘Understanding languages and other cultures builds bridges’. Suzy Kassem Synonyms General Examples Social media has become a big part of popular Traditions culture. Customs People can visit places to learn about other cultures. The arts Scientific Examples We can grow a culture of mould on a piece of bread. Aseptic Technique Petri dishes are often used to produce cultures of bacteria and other microorganisms Introduction The aseptic technique must be used to prepare these cultures This method ensures that the equipment is not contaminated and allows one microorganism to be investigated at a time  Aseptic Technique 1. Petri dishes and culture media (agar) must be sterilised before use 2. Agar in Petri dishes must be set Introduction 3. Inoculating loops are used to transfer the bacteria to the agar but must first be sterilised by passing them through a flame 4. The lid of the Petri dish should be secured with tape and stored upside down 5. In school laboratories the culture should be incubated at 25 °C. Answer the following questions: Check for understanding 1. Why is the aseptic technique used? 2. What is an inoculating loop used for? 3. Why is the inoculating loop passed through a flame first? 4. What temperature should cultures be incubated at? Think outside the box! What would happen if the Petri dish was sealed on all sides by adhesive tape? Talk Task  Drill 1. Explain why the aseptic technique used. Check for understanding 2. State what is an inoculating loop used for. 3. Explain why the inoculating loop passed through a flame first. 4. State the temperature at which should cultures be incubated. 5. State the function of the agar in the petri dish. 6. Explain why it is important not to secure the Petri dish with tape on all sides. 7. Explain why the lid of the Petri dish should be secured with tape and stored upside-down. I: Explain scientific method Example question: To ‘explain’ your answer Writing like a scientist Explain why it is important to: should: a. Not place the inoculation loop on the bench to cool Begin with a scientific b. Place the petri dish at 25°C in a school statement. laboratory instead of 35°C, which Use ‘this means that’, would allow bacteria to grow faster ‘because’ or ‘so’ to link your statement to the Model answer: question. We: Explain scientific method Example question: To ‘explain’ your answer Writing like a scientist When growing one type of bacterium should: on a culture medium, explain why it is important to sterilize the culture Begin with a scientific medium and all the apparatus before statement. use. Use ‘this means that’, Model answer: ‘because’ or ‘so’ to link your statement to the question. We: Explain scientific method Example question: To ‘explain’ your answer A student investigated the effectiveness of four Writing like a scientist different antibiotics. The student used the aseptic should: technique to ensure that only one type of bacterium grew on the agar. Explain two aseptic Begin with a scientific techniques the student should have used. statement. Use ‘this means that’, Model answer: ‘because’ or ‘so’ to link your statement to the question. Answer the questions below. 1. Which best explains why the aseptic technique is used? ❑ A. To ensure that there is no contamination when preparing a culture ❑ B. To ensure that the Petri dish is clean ❑ C. To ensure that all microorganisms are allowed to grow Exit ticket 2. Which is not a feature of the aseptic technique? ❑ A. Sterilising the inoculating loop using a flame ❑ B. Securing the Petri dish lid by making an airtight seal ❑ C. Sterilising the agar before use 3. What is the function of the agar in a Petri dish? ❑ A. To provide nutrients ❑ B. To sterilise the petri dish ❑ C. To destroy any microorganisms  Lesson B3.1.3 Feedback What was good about this lesson? What can we do to improve this lesson? Send us your feedback by clicking this link or by emailing [email protected] Thank you!