Biology Chapter: Aseptic Technique and Cells

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Questions and Answers

What is the primary purpose of the aseptic technique in culturing microorganisms?

To eliminate the need for proper disposal of cultures

To ensure that only one type of microorganism is studied (correct)

To increase the growth rate of microorganisms

To sterilize the environment in which cultures are prepared

What temperature is recommended for incubating cultures in school laboratories?

35 °C

20 °C

25 °C (correct)

30 °C

Why must inoculating loops be sterilized before use?

To ensure they can transfer larger samples

To kill any existing bacteria that may contaminate cultures (correct)

To protect the agar from drying out

To prevent the loop from melting

What happens if the Petri dish is sealed on all sides with adhesive tape?

It promotes the growth of anaerobic bacteria Signup and view all the answers

What is the role of agar in Petri dishes?

To act as a solid medium for bacterial growth Signup and view all the answers

Why should the lid of the Petri dish be stored upside down?

To reduce condensation on the agar surface Signup and view all the answers

Why is it important to not place the inoculating loop directly on the bench to cool?

It may pick up contaminants from the bench Signup and view all the answers

What is the significance of sterilizing Petri dishes and culture media before use?

To eliminate competition from other microorganisms Signup and view all the answers

What is the primary purpose of using aseptic technique in microbiology?

To ensure that there is no contamination when preparing a culture Signup and view all the answers

Which of the following is NOT a feature of aseptic technique?

Securing the Petri dish lid by making an airtight seal Signup and view all the answers

What is the main function of agar in a Petri dish?

To provide a surface for microorganism growth Signup and view all the answers

Which of the following statements best describes how to effectively link scientific statements?

Including transitional phrases like 'this means that' or 'because' Signup and view all the answers

When setting up experiments involving bacteria, why is it crucial to sterilise all apparatus?

To ensure that only desired microorganisms grow Signup and view all the answers

Which practice would NOT be appropriate in an aseptic technique laboratory?

Allowing the Petri dish to remain uncovered during the experiment Signup and view all the answers

Which of the following best explains the importance of linking statements in scientific writing?

To clarify the relationships between ideas and findings Signup and view all the answers

What is the purpose of sterilising the inoculating loop before transferring bacteria?

To prevent the introduction of unwanted microorganisms Signup and view all the answers

What is a key function of mitochondria in eukaryotic cells?

Energy production Signup and view all the answers

Which of the following correctly distinguishes between prokaryotic and eukaryotic cells?

Eukaryotic cells contain a nucleus, prokaryotic cells do not. Signup and view all the answers

Which of the following is an example of a unicellular organism?

Bacteria Signup and view all the answers

Why is the Petri dish not secured with an airtight seal when growing bacteria?

To allow gas exchange with the environment Signup and view all the answers

What is the role of agar medium in the culture of microorganisms?

To provide a solid surface for bacterial growth Signup and view all the answers

Which of the following statements is true about unicellular and multicellular organisms?

Unicellular organisms can perform all life functions independently. Signup and view all the answers

Which best describes the difference between the bacterial culture and social culture?

Bacterial culture refers only to microbial growth, whereas social culture refers to societal practices. Signup and view all the answers

Which of the following best defines the term 'aseptic technique'?

Techniques used to minimize contamination when culturing bacteria Signup and view all the answers

Study Notes

Aseptic Technique

Aseptic technique is used to prevent contamination when growing bacteria and other microorganisms.
Petri dishes are used to create cultures of bacteria and other microorganisms, using aseptic technique.
Equipment must be sterile to ensure one microorganism can be investigated.

Eukaryotic vs. Prokaryotic Cells

Eukaryotic cells have a nucleus and membrane-bound organelles.
Prokaryotic cells lack a nucleus and membrane-bound organelles.

Mitochondria Function

Mitochondria are organelles in eukaryotic cells that produce energy (ATP) for the cell.

Examples of Cells

Eukaryotic cell example: Animal cell
Prokaryotic cell example: Bacteria

Unicellular vs. Multicellular Organisms

Unicellular organisms are single-celled, while multicellular organisms are made of multiple cells.

Unicellular Organism Example

Bacteria

Agar Function

The agar medium provides nutrients for growing organisms (bacteria).

Inoculating Loops

Inoculating loops are used to transfer bacteria to the agar.
Loops are sterilized (passed through a flame) before usage to prevent unwanted contamination.

Petri Dish Lid

Petri dish lids should be secured with tape and stored upside down.
An airtight seal is not used, allowing oxygen in (for the bacteria to grow).

Petri Dish Incubation

Bacteria cultures should be incubated at 25°C (in school labs).

Aseptic Technique Stages

Sterilize Petri dishes and culture media (agar) first.
Set the agar in the Petri dishes.
Sterilize inoculating loops by passing them through a flame.
Transfer bacteria to the agar.
Secure the Petri dish lid with tape and store it upside down.
Incubate cultures at specific temperatures.

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Description

This quiz covers aseptic techniques used in microbiology, the differences between eukaryotic and prokaryotic cells, and the role of mitochondria in energy production. Additionally, it explores the distinctions between unicellular and multicellular organisms, along with examples of each type. Test your understanding of these fundamental biological concepts!