Practical Biochemistry Questions PDF

Practical biochemistry 1-Which of the following is the enzyme responsible to convert chromogen to color complex in glucose oxidase Kit? A -4- Aminoantipyrine B-Quinoneimine c-Peroxidase D-Diazine 2-Which of the following statement is INCORRECT: A-The enzyme glucose oxidase catalyzes the oxidation...

Practical biochemistry 1-Which of the following is the enzyme responsible to convert chromogen to color complex in glucose oxidase Kit? A -4- Aminoantipyrine B-Quinoneimine c-Peroxidase D-Diazine 2-Which of the following statement is INCORRECT: A-The enzyme glucose oxidase catalyzes the oxidation of glucose to gluconic acid and hydrogen peroxide (H202). B-Elevated cholesterol levels may occur with hypothyroidism C-Creatinine is an interfering factor of glucose mesaurment. D-High level of ascorbic acid does not with chromogen for H2O2 in glucose assay. 3- Which of the following increase the serum TG level? A - None B-clofibrate C- Ascorbic acid d-Pregnancy 4-Which of the following is the chemical method of serum urea measurment? A - DiacetylMonoxime (DAM) B-Nessler c- Berthelot D-GLDH (Glutamat dehydrogenase) 5-Which of the following increase the serum phosphate level? A –hypocalcemia B-hyperparathyroidism C-Malnutrition D-Alcoholism 6-Which of the following statement is INCORRECT: A- High amounts of parathyroid hormone leads to high calcium levels and low phosphorus levels. B-Total bilirubin is direct bilirubin + indirect bilirubin C-cholesterol esterase hydrolyse cholesterol ester to cholesterol and free fatty acids. D-Bilirubin is the product of globin. 7-Which of the following can be measured by diazo method? a-Cholesterol B-Uric acid c-Bilirubin D- Urea 8-What is the indirect bilirubin? A - Conjugated bilirubin B- Conjugated bilirubin+ unconjugated bilirubin C- unconjugated bilirubin D - Neonatal bilirubin 9-Which of the following is the acceptable range of serum cholesterol level? A-280 mg/dl B-200 mg/dl C->240 mg/dl D-None of them 10- In which tissue conjugation of bilirubine occur? A-blood B-Kidney C-Liver D-Fat Theoretical biochemistry 1-Which of the following statement is correct? A-Glycolysis occurs when ATP concentration is high B-Glycolysis occurs in mitochondria C-Glycolysis occurs in the presence and absence of oxygen... D-During lactate formation, NAD is reconverted into NADH 2-Which of the following enzyme deficiency leads to hemolytic anemia? A -Pyruvate kinas B-Phosphofructokinase C-Glucokinas D-Muscle phosphorylase 3-Which of the following step is inhibited during arsenate poisoning? A-3-phosphoglycerate mutase B-Enolase C-Pyruvate kinase D-Glyceraldehyde-3-phosphate dehydrogenase 4-Which of the following statement is true about Cori Cycle? A-It involves the transport of lactate from skeletal muscle to the liver for gluconeogenesis B-It involves the transport of lactate from the liver to skeletal tissue for gluconeogenesis C-The Cori cycle involves three tissues muscle, liver, and brain D-It is active during resting stages and in well-fed condition 5-What is the net gain of ATP during the conversion of glucose to pyruvate? A-4 ATP B-2 ATP C-6 ATP D-1 ATP 6-Which of the following hormones is responsible for increasing gluconeogenesis in the liver during prolonged starvation? A-TSH B-Insulin C-Thyroxine D-Glucagon 7-Which of the following enzyme catalyzes the conversion of pyruvate to lactate? A-Lactate dehydrogenase B-Pyruvate reductase C-Pyruvate dehydrogenase D-Lactate reductase 8-Which of the following is not a substrate for gluconeogenesis? A-Acetyl CoA B-Lactate C-Alanine D-Glycerol 9-During the Conversion of Glucose to Pyruvate, Two NADH Molecules are Generated. Which of the Following Steps Generates NADH? A -Conversion of fructose-6-phosphate to fructose-1-6-bisphosphate. B-Conversion of glyceraldehydes-3-phosphate to 1-3-bisphosphoglycerate. C-Conversion of 3-phosphoglycerate to 2-phosphoglycerate. D-Conversion of phosphoenolpyruvate to pyruvate. 10-The lipoprotein lipase is present in the endothelial surfaces of adipose tissues in the heart and it is required for hydrolysis and release of triglycerides from chylomicrons. Which of the apolipoprotein that is present in chylomicron serves as the activator of an enzyme lipoprotein lipase? A-ApoB100 B-ApoE C-ApoCIL, D-ApoB48 11-The key regulatory enzyme of cholesterol synthesis is... A-HMG CoA reductase B-HMG CoA synthase C-HMG CoA lyase D-Mevalonate kinase 12-Which of the following rules regarding the interconversion of fuels are central to the integration of metabolic pathways? A -Fatty acids can be made from and converted to carbohydrates and amino acids. B-Carbohydrates can be made from and converted to amino acids and fatty acids. C-Amino acids can be made from and converted to fatty acids. D-Fatty acids can be made from but not converted to carbohydrates and amino acids. 13-Chylomicron is a type of lipoprotein that transports triglycerides from the intestine to peripheral tissues. Which of the following is an integral apolipoprotein present in a hylomicron? A-Apo B48 B-Apo CII C-ApoE D-Apo B100 14-How many ATPs are formed in case of stearic acid (18-carbon backbone by beta oxidation? A-115 B-110. C-134 D-120 15-The conversion of alpha-ketoglutarate to glutamate and glutamine is catalyzed by. A -Glutamate synthetase and Glutamate dehydrogenase B-Glutamate transaminases C-Glutamate dehydrogenase D-Glutamate dehydrogenase and Glutamine synthetase 16-From where are the two amine groups of urea derived? A-both derived from ammonia B-Both derived from uspartate C-One from ammonia and 1 from aspartate D-both derived from glutamate 17-The long-chain fatty acids get transported through the inner mitochondrial membrane A-as carnitine derivative B-freely C-require sodrum-dependent carrier D-as acyl-CoA derivative 18-Which of the following statement is NOT correct? A-Three ATP molecules are reqired for synthesis uorea B-Carbamoyl phosphate synthetase I is the principal enzyme of urea cycle C-Urea cycle is present in liver D-High concentration of ammonia, stimulates urea cycle, 19-Where are ketone bodies synthesized? A-Adipocytes B-Liver C-Astrocytes D-Brain 20-What is the final product of purine degradation in mammals? A-Guanine B-Inosine C-Uric acid D-Hypoxanthine 21-Name the genetic disorder which is caused by the deficiency of enzyme HGPRT? A-SCID B-Cystic fibrosis C-Down syndrome D-Lesch-Nyhan syndrome 22-The enzyme deficient in Type I Crigler-Najjar Syndrome is... A-UDP glucuronosyl transferase B-delta-aminolevulinic acid synthase I C-Ferrochelatase D-Uroporphyrinogen synthase III 23-Which of the following enzymes of the heme synthesis pathway is found in the cytosol? A-delta-aminolevulinic acid synthase I B-Uroporphyrinogen synthase III C-Protoporphyrinogen oxidase D-Ferrochelatase 24-Familial Hypercholesterolemia is a codominant genetic disorder caused by a mutation of the gene that encodes for: A-Apolipoprotein E B-Apolipoprotein B C-VLDL receptor D-LDL receptor 25-Which lipoprotein transports triglycerides from the intestine to peripheral tissues? A-VLDL B-Chylomicron C-LDI D-HDL 26-Which of the following is not involved in the production of Creatine? A -Glycine B-Arginine C-S-adenosyl methionine D-Alanin 27-Tr graduation of the glucose from the non-carbohydrates precursors called as A-glycolysis B-Glycogenolysis C-Kerb's cycle d-giluconeogenesis