Chromatographic and Spectroscopic Techniques PDF

Chromatographic Techniques Chromatography is usually a technique for separation and/or identification of substances in a mixture. This device is powerful in industrail pharmacy. Some types of chromatography: Thin layer chromatography (TLC). High performance liquid chromatography...

Chromatographic Techniques Chromatography is usually a technique for separation and/or identification of substances in a mixture. This device is powerful in industrail pharmacy. Some types of chromatography: Thin layer chromatography (TLC). High performance liquid chromatography (HPLC). Gas chromatography (GC).  Spectroscopic Techniques Ultraviolet and Visible (UV-VIS) Spectroscopy. Atomic spectroscopy. Infrared spectroscopy. Raman spectroscopy. Nuclear magnetic resonance. Mass spectroscopy.  UV-Visible Spectrosscopy A UV-vis spectrophotometer is an analytical instrument that measures the amount of ultraviolet (UV) and visible light that is absorbed by a sample. It is a widely used technique in chemistry, biochemistry, and other fields, to identify and quantify compounds in a variety of samples. The only requirement for a sample to be absorbed in the UV- Vis region, is to have a chromophore. UV-vis spectrophotometers work by passing a beam of light through the sample and measuring the amount of light that is absorbed at each wavelength. The amount of light absorbed is proportional to the concentration of the absorbing compound in the sample.  UV-Visible Spectrosscopy Draw the Calibration curve for the following data: UV-Visible Spectrosscopy