Instrument Methods of Analysis PDF

Colorimetry spectrophotometer Instrument method of Analysis 1. OPTICAL METHOD OF ANALYSIS ABSORPTION METHOD EMISSION METHOD DISPERSION AND SCATTERING 2. ELECTRICAL METHODS OF ANALYSIS POTENTIOMETRIC ANALYSIS POLOROGRAPHIC ANALYSIS 3. CHROMATOGRAPHIC METHOD OF ANALYSIS  OPTICAL METH...

Colorimetry spectrophotometer Instrument method of Analysis 1. OPTICAL METHOD OF ANALYSIS ABSORPTION METHOD EMISSION METHOD DISPERSION AND SCATTERING 2. ELECTRICAL METHODS OF ANALYSIS POTENTIOMETRIC ANALYSIS POLOROGRAPHIC ANALYSIS 3. CHROMATOGRAPHIC METHOD OF ANALYSIS  OPTICAL METHOD OF ANALYSIS MEASURE INTERACTION BETWEEN RADIANT ENERGY AND MATTER. ABSORPTION  UV spectroscopy-low concentration of organic compound such as benzene ring containing compounds, unsaturated straight chain compounds. Infrared spectroscopy- TOC. EMISSION METHOD ATOMIC EMISSION SPECTROSCOPY Atomic absorption spectrophotometer flourimeter Potentiometric POLOROGRAPHIC  Anodic stripping voltammetry is direct polarographic analysis of water and wastewater. Lead cadmium can be measured.  CHROMATOGRAPHY Set of different procedure used to separate components in a mixture based on their relative affinity towards partitioning between two phase. Two different phases are used: STATIONARY PHASE AND MOBILE PHASE. STATIONARY PHASE IS EITHER LIQUID OR SOLID  WHEN the mobile phase is gas – Gas chromatography. When it is liquid – liquid chromatography Mobile phase is gas and stationary phase is liquid- gas liquid chromatography. Mobile phase is liquid and stationary phase is paper- paper chromatography  Gas chromatography Volatile organic compounds, pesticide analysis  Column: HPLC  ION CHROMATOGRAPHY VARIATION OF HPLC Analytical column consists of ion exchange resin.  Electrophoresis Separation process- charged species migrate at different rates in an electric field. Used to separate variety of materials form drugs food, natural products, biological materials and pesticides. “Standard methods”-analysis of common inorganic ion such as fluoride, chloride, sulfate, nitrate , nitrite, bromide and acetate. Competitive method to ion chromatography  Other instrument Mass spectrometry: Used in conjunction with GC, LC Large number of inorganic and organic compounds present in water and wastewater.