Practice Questions for Spectroscopy in Biology PDF
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This document is a collection of practice questions covering various spectroscopy techniques in biology, including mass spectrometry, IR spectroscopy, chromatography, and UV/Vis spectroscopy. The questions assess understanding of key concepts and principles related to these techniques. These practice questions will help solidify knowledge about the different forms of spectroscopy used.
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Practice Questions for Spectroscopy in Biology – Unit 4 (1) Which of the following statements is NOT true regarding Mass Spectrometry? (a) A mass spectrometer detects ions of molecules. (b) A mass spectrometer cannot be used to detect proteins. (c) Ions must be in the gas phase to be...
Practice Questions for Spectroscopy in Biology – Unit 4 (1) Which of the following statements is NOT true regarding Mass Spectrometry? (a) A mass spectrometer detects ions of molecules. (b) A mass spectrometer cannot be used to detect proteins. (c) Ions must be in the gas phase to be detected by a mass spectrometer. (d) A mass spectrometer can detect different isotopes of a molecule. (e) A mass spectrometer can be used to find the molecular mass of a compound. (2) Which of the following statements in NOT true regarding Infra-Red (IR) Spectroscopy? (a) The peaks arise out of electronic transitions in the molecule. (b) The spectrum can tell you what functional groups are in the molecule. (c) The peaks arise out of bond vibrations within in the molecule. (d) IR Spectroscopy can provisionally confirm identity of a molecule. (e) The peaks arise when Infra-Red radiation is absorbed by a molecule. (3) Which of the following statements about chromatography is INCORRECT? a) Chromatography uses a stationary phase and a mobile phase b) Thin Layer Chromatography (TLC) is a qualitative technique c) In Gas Liquid Chromatography (GLC) the mobile phase does not contribute to separation d) Chromatography cannot separate out large biological molecules e) Different components of a mixture are separated due to having different retention times (4) When comparing properties of UV/Vis (Ultraviolet/Visible) Spectroscopy and Fluorescence Spectroscopy, which of the following statements is CORRECT? (a) Fluorescence spectroscopy measures absorbed radiation whereas UV/Vis spectroscopy measures emitted radiation. (b) More compounds are fluorescent than are active in UV / Vis. (c) Both techniques involve changes in electronic energy levels within a molecule. (d) Fluorescence Spectroscopy is a less sensitive technique than UV/Vis Spectroscopy. (e) Only UV/Vis Spectroscopy is commonly used for quantitative analysis. (5) How many carbon signals to you expect to see in the 13C NMR spectrum of 4-methoxy benzaldehyde (see below)? (a) 2 (b) 3 (c) 4 (d) 5 (e) 6
