2Nd Year PUC Practical Examination Biology PDF 2023-24

2023-24 2ND YEAR PUC PRACTICAL EXAMINATION SUBJECT: BIOLOGY (36) EXPERIMENTS TO BE CONDUCTED IN PRACTICALS FOR THE YEAR 2023-24 Exercise 01: To study the reproductive parts of commonly available flowers Exercise 02: To calculate percentage of pollen germinat...

2023-24 2ND YEAR PUC PRACTICAL EXAMINATION SUBJECT: BIOLOGY (36) EXPERIMENTS TO BE CONDUCTED IN PRACTICALS FOR THE YEAR 2023-24 Exercise 01: To study the reproductive parts of commonly available flowers Exercise 02: To calculate percentage of pollen germination. Exercise 03: To study pollen tube growth on stigma. Exercise 04: To study the discrete stages of gametogenesis in mammalian testis and ovary Exercise 05: To study and identify various stages of female gametophyte development in the ovary of a flower Exercise 06: Preparation and study of mitosis in onion root tips Exercise 07: Study of stages of meiosis using permanent slides Exercise 08: To study the blastula stage of embryonic development in mammals, with the help of permanent slide, chart, model or photograph Exercise 09: To verify Mendel's Law of Segregation Exercise 10: To verify the Mendel’s Law of Independent Assortment Exercise11: Preparation and analysis of Pedigree Charts Exercise 12: To perform emasculation, bagging and tagging for controlled pollination Exercise 13: Staining of nucleic acid by acetocarmine Exercise 14: To identify common disease-causing organisms and the symptoms of the diseases Exercise 15: To study the texture of soil samples Exercise 16: To determine the water-holding capacity of soils Exercise 17: To study the ecological adaptations in plants living in xeric and hydric conditions Exercise 18: To study the adaptations in animals living in xeric and hydric conditions 1 Exercise 19: To determine the pH of different water and soil samples Exercise 20: To analyse living organisms in water samples Exercise 22: To determine the amount of Suspended Particulate Matter (SPM) in air at different sites in a city Exercise 23: To study plant population density by quadrant method Exercise 24: To study plant population frequency by quadrant method Exercise 25: Study of homologous and analogous organs in plants and animals Investigatory Project Work ---- 2  2023-24 2ND YEAR PUC PRACTICAL EXAMINATION SUBJECT: BIOLOGY (36) QUESTION PAPER TIME: 2 HOURS MAXIMUM MARKS: 30 1. Prepare a temporary slide to show pollen germination from the given material “A1” and calculate the percentage of pollen germination. OR Prepare a temporary slide to show pollen tube growth on the stigma from the given material “A2” and draw a labeled diagram of your observation. OR Prepare a temporary slide of given material “A3” by taking a transverse section of ovary and report the number of locules and type of placentation. OR Prepare a temporary slide to show nucleic acid staining from the given material “A4” and report the shape of the cell and number of nuclei observed. 5 marks 2. Prepare a temporary slide of the given material “B” and identify any one stage of mitosis and comment on the stage observed. 5 marks 3. Identify and comment on the given pedigree chart “C”. 3 marks 4. Identify “D” and comment. 3 marks 5. Identify “E”and comment. 2 marks 6. Identify “F”and comment. 2 marks 7. Viva voce 4 marks 8. Practical Records 6 marks 3  ND 2023-24 2 YEAR PUC PRACTICAL EXAMINATION SUBJECT: BIOLOGY (36) SCHEME OF EVALUATION TIME: 2 HOURS MAXIMUM MARKS: 30 QUE.NO SUBJECT SCHEME OF EVALUATION NOTE TO EXAMINERS 1* Pollen germination experiment Preparation of the slide 3 marks *Any one of the experiments to be performed by the student on the Calculating the % pollen basis of selection by lottery system. Of the four experiments, one germination 2 marks will be selected by each student. Pollen tube growth on stigma Preparation of the slide 3 marks All the materials for each and every experiment should be made experiment Labeled diagram 2marks available for the student. Transverse Section of ovary Preparation of the slide 3 marks Only raw material has to be provided. Conduction of experimental Reporting the number of locules procedures such as sectioning, staining, slide preparation, etc., in ovary 1 mark should be done by the student only, wherever necessary. Reporting the type of placentation 1 mark Nucleic acid staining experiment Preparation of the slide 3 marks Reporting the shape of the cell- 1 mark Reporting the number of nuclei 1 mark 2 Prepare a temporary mount of onion Preparation of the slide 3 marks Note: Root tip should be provided to the student. Processing of root root tip to study mitosis. Identifying any one stage 1 mark tip such as treatment, staining, slide preparation etc., should be Commenting on the stage done by the student only. observed 1 mark 3 Pedigree Chart Identification of type pedigree* Pedigree chart analysis 1 mark One of the Pedigree charts prepared during practical classes is to be given. Comments 2 marks *Autosome Linked Dominant traits 4  *Autosomal Recessive traits *X-linked Dominant traits *X-linked Recessive traits *Y-chromosome linked traits 4 Identification and commenting Identification 1 mark One of the following has to be given for question no.4 Comments 2 marks Slide showing T.S of testis/ T.S of ovary/ V.S of ovary showing female gametophyte/V.S of blastula. (If the slides are not available, then only suitable photograph/model that were shown in the practical classes can be given for identification and commenting) 5 Identification and commenting on the Identification with its scientific One of the following has to be given for question no.5 disease causing organisms name 1 mark Common disease causing organisms like Ascaris, Entamoeba*, Comments 1 mark Plasmodium*, Ring-worm fungus(Trichophyton rubrum*) through permanent slides, models or virtual images or specimens. (If the slides* are not available, then only suitable models or virtual images or specimen that were shown in the practical classes can be given for identification and commenting). 6 Identification and commenting Identification 1 mark One of the following has to be given for question no.6 Comments 1 mark i) Controlled pollination - emasculation, tagging and bagging ii) Homologous and analogous organs 1. Homologous Organs in Plants (i) Tendrils of passion flower and thorns of pomegranate (ii) Tendrils of Vitis and thorns of Carissa (iii) Tendrils of baloon vine (Cardiospermum) and bulbils of Agave. (iv) Scale leaves of onion and spines of prickly pear (Opuntia) 2. Analogous Organs in Plants (i) Stem tendrils and leaf tendrils (ii) Thorns and spines 5  (iii) Modified underground stems and modified roots (iv) Phylloclade, cladode and leaves 3. Homologous Organs in Animals Wings of birds, and forelimb of mammals/reptiles/frog: All have the same bony elements (humerus, radio-ulna, carpals, metacarpals and phalanges), but perform different (flying in birds, for holding or walking etc. in other) functions. 4. Analogous Organs in Animals (i) Wings of dragonfly/cockroach/butterfly and of birds. (ii) Mandible of cockroach and mandible (lower jaw) of a vertebrate. *(If the specimens not available, then only Flash cards /specimen/models showing examples of can be given for identification and commenting) 7 Viva Voce 4 marks Questions pertaining to the experiments given in that particular batch of practical exam only to be asked. Viva Voce should be conducted in the presence of both external and internal examiner. 8 Practical record 6 marks Refer the table below* *Marks to be allotted to practical record Sl.No No. of experiments to be Maximum Marks performed and recorded 1 Less than 75% of exercises Not eligible 2 75% to 79% of exercises* 3 3 80% to 84% of exercises* 4 4 85% to 89% of exercises* 5 5 More than 90% of exercises* 6 *Investigatory projects must be considered for evaluation. 6