17 Questions
What is the primary function of Mercuric Chloride fixatives?
Applied in Ultrathin Sections
What are the disadvantages of formaldehyde fixatives mentioned in the text?
Can cause allergic dermatitis
When is a concentration of 4% formaldehyde usually utilized?
For fixing larger tissues
Which type of fixative is often used for Central Nervous System (CNS) tissues?
Lead Fixatives
What is a common function of Chromate Fixatives?
Primarily used in Electron Microscopy
Which fixative should not contain Osmic acid?
Heidenhain's Susa
Which fixative is known for preserving the nucleus and chromatin material of the cell?
Flemming's Fixative
Which fixative renders the pH ≤4.6 and preserves membrane-bound organelles?
Formol Corrosive
Which fixative should not contain glacial acetic acid?
Glutaraldehyde
Which fixative is used to determine fat contents, presence of enzymes, and mucopolysaccharides?
Newcomer's Bouin's Fixative
Which fixative is an aldehyde fixative?
Formol Corrosive
What is the most commonly utilized fixative in the laboratory?
Formalin
What is the purpose of using phosphate buffers in formalin fixation?
To stabilize the pH at 7
What component is used to dilute Formaldehyde to make a 10% solution for routine processing?
NaCl
In which fixative do we see the use of Picric Acid and Glacial Acetic Acid together?
HISTO 10% Formol Saline
Which fixative is known as 'Formol sublimate' and contains Chloride?
Orth's Regaud's Fixative
At what concentration is Glutaraldehyde often used for small tissues?
2.5%
Study Notes
Fixatives
- Mercuric Chloride fixatives' primary function is to fix and preserve tissues.
- Formaldehyde fixatives have disadvantages, including being toxic and carcinogenic, and causing tissue shrinkage and hardening.
- A 4% concentration of formaldehyde is usually utilized for routine tissue fixation.
- Glutaraldehyde is often used for fixing Central Nervous System (CNS) tissues.
- Chromate Fixatives are used to preserve lipids and membranes.
- Osmic acid should not be used in Formaldehyde fixatives.
- Formaldehyde is known for preserving the nucleus and chromatin material of the cell.
- Acetic acid fixatives render the pH ≤4.6 and preserve membrane-bound organelles.
- Glacial acetic acid should not be used in Formaldehyde fixatives.
- Fat-containing fixatives, such as OsO4, are used to determine fat contents, presence of enzymes, and mucopolysaccharides.
- Formaldehyde is an aldehyde fixative.
- The most commonly utilized fixative in the laboratory is Formaldehyde.
- Phosphate buffers are used in formalin fixation to stabilize the pH and prevent tissue autolysis.
- Distilled water is used to dilute Formaldehyde to make a 10% solution for routine processing.
- Bouin's fixative uses Picric Acid and Glacial Acetic Acid together.
- Formol sublimate, which contains Chloride, is another name for Mercuric Chloride fixative.
- Glutaraldehyde is often used at a concentration of 2-3% for small tissues.
Test your knowledge on fixatives used in histopathology, including cytocological and histochemical fixatives. Learn about the different types of fixatives and their specific actions and remarks.
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