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5 Questions

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Histopathology: Fixation Process

Created by

@SweetheartHarpsichord

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Questions and Answers

What is the main aim of fixation in tissue preservation?

To maintain the tissue in a semi-solid state (correct)

To liquefy the tissue

To allow bacterial action on the tissue

To produce a foul odor in the tissue

What is the effect of fixation on the tissue?

Causes liquefaction with a foul odor

Promotes tissue decomposition

Induces bacterial action on the tissue

Maintains the tissue in a semi-solid state (correct)

What would happen if fresh tissue is kept at room temperature without fixation?

It will become dry and shrunken

It will become liquefied with a foul odor due to bacterial action (correct)

It will remain in a semi-solid state

It will repel bacterial action

Why is preservation of tissue in a semi-solid state important?

<p>To make the tissue easier to handle</p> Signup and view all the answers

What is the role of fixation in preventing putrefaction and autolysis?

<p>To maintain the tissue in a semi-solid state</p> Signup and view all the answers

Study Notes

Fixation in Histopathology

Fixation is a complex series of chemical events that bring about changes in the various chemical constituents of cells, resulting in hardening, while preserving cell morphology and structural detail.
It is essential to fix tissues soon after removal from the body to prevent degenerative changes due to autolysis and putrefaction, which would lead to loss of individual cell morphology.

Principle of Fixation

The fixative brings about crosslinking of proteins, resulting in denaturation or coagulation of proteins.
This process converts the semifluid state of cells into a semisolid state, maintaining the in vivo relationships between cells.
The semisolid state facilitates easy manipulation of tissue, making it ideal for histopathological analysis.

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Description

Learn about the complex process of fixation in histopathology, which involves chemical events to preserve cell morphology and structural detail. Understand the importance of timely tissue fixation to prevent degenerative changes and maintain tissue morphology.