What is the Roll tube method?
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The question is asking about the 'Roll tube method', which is likely a specific technique or process used in a particular field. The user is seeking information or details regarding this method.
Answer
Distributing agar medium inside a test tube under anaerobic conditions for anaerobic bacteria isolation.
The roll-tube method involves distributing agar medium as a thin layer over the inside surface of a test tube under an anaerobic atmosphere, mainly used for isolating obligately anaerobic bacteria.
Answer for screen readers
The roll-tube method involves distributing agar medium as a thin layer over the inside surface of a test tube under an anaerobic atmosphere, mainly used for isolating obligately anaerobic bacteria.
More Information
The method is particularly useful for studying obligate anaerobes, which are difficult to culture using standard techniques that do not exclude oxygen.
Tips
A common mistake is not maintaining strict anaerobic conditions, which can kill obligate anaerobes.
Sources
- The roll-tube method - sciencedirect.com
- Improved isolation of anaerobic bacteria - pubmed.ncbi.nlm.nih.gov
- The Roll-Tube Method for Cultivation of Strict Anaerobes - jstor.org
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