What is cell culture? What is the difference between primary culture and sub-culture? What are the steps of the cell culture process? Explain the following of how each type of cell... What is cell culture? What is the difference between primary culture and sub-culture? What are the steps of the cell culture process? Explain the following of how each type of cell looks like: Fibroblast-like, epithelial-like, lymphoblast-like. What is the temperature for optimal growth of human and mammalian cell line?
Understand the Problem
The question is asking for definitions and differences in cell culture techniques, as well as specifics about the characteristics of different cell types and optimal growth temperature for human and mammalian cell lines.
Answer
Cell culture is the growth of cells in controlled conditions. Primary and sub-cultures differ in the initial isolation and transfer for continued growth. Steps are isolation, sterilization, growth, sub-culture, and harvesting. Cell types look fibroblast-like (elongated), epithelial-like (polygonal), and lymphoblast-like (round). Optimal temperature: 37°C.
Cell culture is the process of growing cells in controlled conditions. Primary culture involves the initial growth of cells taken directly from tissues, whereas sub-culture involves transferring cells from one vessel to another for continued growth. The steps of cell culture include isolation, sterilization, growth, sub-culture, and harvesting. Fibroblast-like cells are elongated and spindle-shaped, epithelial-like cells are polygonal and tightly packed, and lymphoblast-like cells are round and in suspension. The optimal temperature for the growth of human and mammalian cell lines is 37°C.
Answer for screen readers
Cell culture is the process of growing cells in controlled conditions. Primary culture involves the initial growth of cells taken directly from tissues, whereas sub-culture involves transferring cells from one vessel to another for continued growth. The steps of cell culture include isolation, sterilization, growth, sub-culture, and harvesting. Fibroblast-like cells are elongated and spindle-shaped, epithelial-like cells are polygonal and tightly packed, and lymphoblast-like cells are round and in suspension. The optimal temperature for the growth of human and mammalian cell lines is 37°C.
More Information
Cell culture is a fundamental technique in biological research and biotechnology. Understanding cell morphologies helps in identifying cell types and their state of health.
Tips
A common mistake is confusing primary culture with sub-culture. Remember that primary culture is the initial step directly from tissues, while sub-culture is about transferring cells to keep them growing.
Sources
- Introduction to Cell Culture | Thermo Fisher Scientific - US - thermofisher.com
- CELL CULTURE BASICS Handbook - vanderbilt.edu
- Primary Cell Culture Basics - Sigma-Aldrich - sigmaaldrich.com
