Podcast
Questions and Answers
What staining technique is used to visualize mycobacteria in specimens?
What staining technique is used to visualize mycobacteria in specimens?
At what concentration of mycobacteria per ml can they be visualized under direct microscopy?
At what concentration of mycobacteria per ml can they be visualized under direct microscopy?
How do mycobacteria appear when stained with Ziehl-Neelsen stain?
How do mycobacteria appear when stained with Ziehl-Neelsen stain?
Which type of tuberculosis usually yields a large number of mycobacteria in specimens?
Which type of tuberculosis usually yields a large number of mycobacteria in specimens?
Signup and view all the answers
What is the colonial morphology of M.tuberculosis on glycerol-containing media described as?
What is the colonial morphology of M.tuberculosis on glycerol-containing media described as?
Signup and view all the answers
Which fluorescent dye can be used to examine mycobacteria under a UV microscope when small numbers are present?
Which fluorescent dye can be used to examine mycobacteria under a UV microscope when small numbers are present?
Signup and view all the answers
What is the term used to describe the sparse, thin growth of M.bovis on glycerol-containing media?
What is the term used to describe the sparse, thin growth of M.bovis on glycerol-containing media?
Signup and view all the answers
Which type of mycobacteria produces yellowish-orange carotenoid pigments?
Which type of mycobacteria produces yellowish-orange carotenoid pigments?
Signup and view all the answers
What is the term applied to mycobacteria that produce pigment only if exposed to light?
What is the term applied to mycobacteria that produce pigment only if exposed to light?
Signup and view all the answers
What is one procedure for isolating and cultivating mycobacteria from clinical material like nodules?
What is one procedure for isolating and cultivating mycobacteria from clinical material like nodules?
Signup and view all the answers
What is the principal basis for identifying mycobacteria?
What is the principal basis for identifying mycobacteria?
Signup and view all the answers
What method is being used to replace the complex and time-consuming phenotypic approach for identifying mycobacteria?
What method is being used to replace the complex and time-consuming phenotypic approach for identifying mycobacteria?
Signup and view all the answers
What is the purpose of the acid-fast stain technique?
What is the purpose of the acid-fast stain technique?
Signup and view all the answers
Why must the stain (carbol fuchsin) be driven into the cells with heat in the acid-fast stain technique?
Why must the stain (carbol fuchsin) be driven into the cells with heat in the acid-fast stain technique?
Signup and view all the answers
What makes acid-fast cell walls more durable compared to other bacteria?
What makes acid-fast cell walls more durable compared to other bacteria?
Signup and view all the answers
How are non acid-fast bacteria affected by decolorization with acid-alcohol in the staining process?
How are non acid-fast bacteria affected by decolorization with acid-alcohol in the staining process?
Signup and view all the answers
What is the primary method used to differentiate between acid-fast and non acid-fast bacteria after staining?
What is the primary method used to differentiate between acid-fast and non acid-fast bacteria after staining?
Signup and view all the answers
How does mycolic acid contribute to the pathogenicity of some bacteria?
How does mycolic acid contribute to the pathogenicity of some bacteria?
Signup and view all the answers
