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Questions and Answers
What staining technique is used to visualize mycobacteria in specimens?
What staining technique is used to visualize mycobacteria in specimens?
- Ziehl-Neelsen staining (correct)
- Auramine staining
- Gram staining
- Acid-fast staining
At what concentration of mycobacteria per ml can they be visualized under direct microscopy?
At what concentration of mycobacteria per ml can they be visualized under direct microscopy?
- 5 x 105
- 5 x 106
- 5 x 104 (correct)
- 5 x 103
How do mycobacteria appear when stained with Ziehl-Neelsen stain?
How do mycobacteria appear when stained with Ziehl-Neelsen stain?
- Green rods on a white background
- Red rods on a blue background (correct)
- Yellow rods on a black background
- Blue rods on a red background
Which type of tuberculosis usually yields a large number of mycobacteria in specimens?
Which type of tuberculosis usually yields a large number of mycobacteria in specimens?
What is the colonial morphology of M.tuberculosis on glycerol-containing media described as?
What is the colonial morphology of M.tuberculosis on glycerol-containing media described as?
Which fluorescent dye can be used to examine mycobacteria under a UV microscope when small numbers are present?
Which fluorescent dye can be used to examine mycobacteria under a UV microscope when small numbers are present?
What is the term used to describe the sparse, thin growth of M.bovis on glycerol-containing media?
What is the term used to describe the sparse, thin growth of M.bovis on glycerol-containing media?
Which type of mycobacteria produces yellowish-orange carotenoid pigments?
Which type of mycobacteria produces yellowish-orange carotenoid pigments?
What is the term applied to mycobacteria that produce pigment only if exposed to light?
What is the term applied to mycobacteria that produce pigment only if exposed to light?
What is one procedure for isolating and cultivating mycobacteria from clinical material like nodules?
What is one procedure for isolating and cultivating mycobacteria from clinical material like nodules?
What is the principal basis for identifying mycobacteria?
What is the principal basis for identifying mycobacteria?
What method is being used to replace the complex and time-consuming phenotypic approach for identifying mycobacteria?
What method is being used to replace the complex and time-consuming phenotypic approach for identifying mycobacteria?
What is the purpose of the acid-fast stain technique?
What is the purpose of the acid-fast stain technique?
Why must the stain (carbol fuchsin) be driven into the cells with heat in the acid-fast stain technique?
Why must the stain (carbol fuchsin) be driven into the cells with heat in the acid-fast stain technique?
What makes acid-fast cell walls more durable compared to other bacteria?
What makes acid-fast cell walls more durable compared to other bacteria?
How are non acid-fast bacteria affected by decolorization with acid-alcohol in the staining process?
How are non acid-fast bacteria affected by decolorization with acid-alcohol in the staining process?
What is the primary method used to differentiate between acid-fast and non acid-fast bacteria after staining?
What is the primary method used to differentiate between acid-fast and non acid-fast bacteria after staining?
How does mycolic acid contribute to the pathogenicity of some bacteria?
How does mycolic acid contribute to the pathogenicity of some bacteria?
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