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Veterinary Medicine: Phosphorus and Nutrient Absorption

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What is the main factor affecting the availability of phosphorus in food?

Presence of phytases

What happens to the availability of mineral elements as an animal's diet changes from milk-based to solid foods?

It declines

What is the primary purpose of metabolizing proteins, carbohydrates, and lipids in animal feed?

To synthesize animal products like eggs and milk

What type of energy conversion occurs during the metabolism of proteins, carbohydrates, and lipids?

Chemical energy to chemical energy

What is the primary source of phosphorus in many foods?

Phytic acid

What is the significance of phytases in phosphorus availability?

They break down phytic acid, increasing phosphorus availability

What is the formula to calculate the efficiency of ME utilization (k)?

k = Net Energy output/ME intake

What is a factor that affects the variation of 'k' values?

The process for which ME is being used

What is the reason for ruminants having lower 'k' values than non-ruminants?

Heat of fermentation

Which type of foods tend to have higher 'k' values?

Foods with high ME values

What is the purpose of the subscript letter in 'k' values?

To indicate the function for which ME is being used

What is the source of the information about 'k' values?

McDonald et al. (2022). Animal Nutrition, 8th ed.

What is the primary consideration when formulating diets for animals?

Satisfying the animals' energy requirements

What is the system used for ruminants in the British system?

Metabolisable energy system

What is the unit of measurement for net energy requirement for growth?

MJ/kg

What is the formula to calculate the efficiency of ME utilization?

k = NE output / ME intake

If an animal growing at 1.0 kg/day stores 15.0 MJ of energy, what is its net energy requirement for growth?

15.0 MJ/kg

What is the purpose of the additional factor in the Blaxter system?

To account for the variation in energy utilization

If food has an ME content of 10.0 MJ/kg and the efficiency of ME utilization for growth is 0.5, what is the NE content of the food?

5.0 MJ/kg

If the food to be fed contains 5.0 MJ/kg (NE), how much quantity is required to meet the animal's energy requirement?

3.0 kg

What is the basis for the efficiency of Metabolisable Energy (ME) utilization?

The metabolisability (qm) of the diet

What is the value of kg in relation to km for high-quality foods?

74% of km

What is the unit of measurement for Metabolisable Energy (ME)?

No specific unit mentioned

What is the purpose of using efficiency factors (k) in animal nutrition?

To calculate the nutritional requirements of animals

What is the relationship between ME and BE in the equation 𝑞𝑚 = 𝑀𝐸 / 𝐵𝐸?

ME is directly proportional to BE

What is the reference for the equation to predict the efficiencies of ME utilization?

McDonald et al. (2022)

What is the value of kg in relation to km for low-quality foods?

34% of km

What is the purpose of calculating the ME requirements of animals?

To ensure the optimal energy supply for different productive processes

What is the formula for RDP (Rapidly Degraded Protein) in terms of CP (Crude Protein)?

RDP = a x CP

What is the purpose of the nylon bag technique in determining the degradability of a food?

To determine the rapidly degradable fraction of a food

What is the meaning of the parameter 'a' in the formula for RDP and SDP?

Rapidly degradable fraction

What is the formula for ERDP (Effective Rumen Degradable Protein)?

ERDP = CP x [0.8 a + (c / (c + r))]

What is the meaning of the parameter 'c' in the formula for RDP and SDP?

Utilization yield by microorganisms

What is the purpose of determining the degradability of a food?

To determine the nutritional value of a food for ruminants

What is the volume of one gram-molecule of oxygen?

22.4 litres

If an animal obtains all its energy from the oxidation of glucose, what is the thermal equivalent of oxygen?

20.98 kJ/litre

What is the average thermal equivalent of oxygen for a mixture of carbohydrates?

21.12 kJ/litre

Why is it necessary to have different approaches to evaluate protein sources in animals?

Because the nitrogen requirements of animals and the nitrogen status of foods are stated in terms of protein

What is the thermal equivalent of oxygen for lipids?

19.61 kJ/litre

What is the primary purpose of measuring the protein content of a food?

To ensure the food provides sufficient essential and nonessential amino acids

What is the thermal equivalent of oxygen for proteins?

18.8 kJ/litre

What is the energy production from 1 litre of oxygen when an animal obtains all its energy from the oxidation of glucose?

20.98 kJ

What is the basis for calculating the protein content of a food?

The nitrogen content of the food determined by the Kjeldahl technique or Dumas method

How do animals normally obtain energy?

From a mixture of carbohydrates, lipids, and proteins

What is the significance of essential and nonessential amino acids in animal nutrition?

They are necessary for the synthesis of protein

What is the significance of thermal equivalents of oxygen in animal nutrition?

To determine the energy production from oxygen

Why is protein synthesis important in animal nutrition?

Because most of the nitrogen required by the animal is used for protein synthesis

What is the relationship between the nitrogen content of a food and its protein content?

The protein content is calculated from the nitrogen content

What is the purpose of evaluating protein sources in animal nutrition?

To ensure the food provides sufficient essential and nonessential amino acids

Why is it necessary to consider the type of animal being fed when evaluating protein sources?

Because the nitrogen requirements of animals and the nitrogen status of foods are stated in terms of protein

What is the current limitation of mechanistic models in predicting animal requirements and responses to changes in nutrient supply?

Inability to accurately predict energy values of foods

What happens to protein in the rumen of ruminant animals?

It undergoes considerable degradation and synthesis

Why do mechanistic models include equations for predicting energy values of foods?

Because energy values are variable and depend on chemical composition

How do simple-stomached animals obtain amino acids from food?

Through breakdown of food proteins during digestion and absorption

What is the purpose of determining the degradability of a food in sacco studies?

To determine the rapidly degradable, slowly degradable, and undegradable fractions of a food

Why is it important to consider the chemical composition of foods in animal nutrition?

All of the above

What is the unit of measurement for RDP (Rapidly Degraded Protein)?

g/kg DM

What is the formula for SDP (Slowly Degraded Protein) in terms of CP (Crude Protein)?

SDP = b x CP / (c + r)

What is the expected outcome of using computers in animal nutrition in the future?

More accurate prediction of animal requirements

What is the meaning of the parameter 'b' in the formulas for RDP and SDP?

Slowly degradable fraction

What is the formula for ERDP (Effective Rumen Degradable Protein)?

ERDP = CP x [0.8a + (c+r) / (c+r)]

What is the purpose of the nylon bag technique in determining the degradability of a food?

To determine the rapidly degradable, slowly degradable, and undegradable fractions of a food

What is the basis for the Essential Amino Acid Index (EAAI)?

The geometric mean of the ratios of the essential amino acids

What are the two sources of protein for ruminant animals?

Microbial and food

What is the advantage of using the Essential Amino Acid Index (EAAI)?

It predicts the effect of supplementation in combinations of proteins

What is the relationship between the Chemical/Amino Acid Score and the Essential Amino Acid Index (EAAI)?

They are both measures of protein quality based on amino acid composition

What is the reference pattern used for the Essential Amino Acid Index (EAAI)?

The pattern of essential amino acids in egg protein

What is the unit of measurement for protein quality?

There is no unit of measurement for protein quality

What is the purpose of measuring protein quality?

To evaluate the nutritional value of protein sources

What is the difference between the Chemical/Amino Acid Score and the Essential Amino Acid Index (EAAI)?

The Chemical/Amino Acid Score is based on the ratios of all amino acids, while the EAAI is based on the ratios of the essential amino acids

Study Notes

Mineral Availability

  • Phosphorus (P) is present in many foods as a constituent of phytic acid, and its availability depends on the presence of phytases of animal, microbial, or exogenous origin.
  • Mineral availability is generally high in young animals fed on milk-based diets, but generally declines as the diet changes to solid foods (trapped in food matrix).

Energy Content and Partition

  • Energy from feed is used to synthesize animal products like eggs and milk through metabolism of proteins, carbohydrates, and lipids.
  • Efficiency of ME (Metabolizable Energy) utilization (k) varies depending on animal species, process for which ME is being used, and nature of ME supply (= feed type).
  • Foods with high ME values tend to have higher k values because they are digested and metabolized with less energy expenditure.
  • Ruminants have lower k values than non-ruminants, mainly because of the heat arising from microbial metabolism (heat of fermentation).

Diets Formulation

  • First, ensure that the animals' energy requirements are satisfied.
  • Second, maintain the correct balance between energy and other nutrients.

Systems to Express Feed Energy Supply

  • The British system used for ruminants is based on Metabolizable Energy (ME) and considers an additional factor, the efficiency of ME utilization (k).
  • Animal requirements are expressed in terms of Net Energy (NE).
  • Food energy values are expressed as Metabolizable Energy (ME).
  • Efficiency factors (k) are used for different productive processes (e.g., maintenance, lactation, and growth), which depend on the ME concentration of the diet (ME/DE).

Blaxter System

  • Animal ME requirements are calculated using efficiency factors (k) for different productive processes.
  • For low-quality foods (qm = 0.4), kg is only 34% of km.
  • For high-quality foods (qm = 0.7), kg is 74% of km.

Efficiency of Metabolizable Energy (ME) Utilization

  • The efficiency of ME utilization (k) is based on the metabolisability (qm).
  • ME utilization (k) is predicted using a series of equations.

RDP (Rumen Degradable Protein)

  • RDP (g/kg DM) = a x CP, where a is the rapidly degradable fraction (water-soluble N).
  • SDP (g/kg DM) = bc x CP, where b is the slowly degradable fraction (N potentially degraded).
  • ERDP = CP x [0.8 a + (c+r) / (c+r)], where c is the utilization yield by m.o.

Oxidation of Glucose

  • One gram-molecule of oxygen has a volume of 22.4 liters (normal temperature and pressure)
  • The overall reaction for oxidation of glucose is: C6H12O6 + 6O2 -> 6CO2 + 6H2O + 2820 kJ
  • The utilization of 1 liter of oxygen leads to the production of 2820/ (6 x 22.4) = 20.98 kJ heat

Thermal Equivalents of Oxygen

  • Carbohydrates: 21.12 kJ/ liter
  • Lipids: 19.61 kJ/ liter
  • Proteins: 18.8 kJ/ liter

Energy Content and Partition

  • Animals do not normally obtain energy exclusively from carbohydrate or fat, but tend to oxidize a mixture of these (and protein)
  • Mechanistic models may be able to predict animal requirements and responses to changes in nutrient supply in the future
  • The energy value of some foods is variable and can be predicted from their chemical composition

Measurements of Food Protein

  • Simple-stomached animals obtain amino acids from the breakdown of food proteins during digestion and absorption
  • Ruminant animals have a more complex situation, with considerable degradation and synthesis of protein occurring in the rumen
  • Different approaches are necessary for evaluating protein sources for ruminant and non-ruminant animals

Protein Evaluation

  • The nitrogen requirements of animals and the nitrogen status of foods are stated in terms of protein
  • The protein content of a food is calculated from its nitrogen content determined by a modification of the classical Kjeldahl technique or the Dumas method

Amino Acid Profile

  • The amino acid profile of a food is important for its efficient use by the animal
  • Essential amino acid index (EAAI) is the geometric mean of the egg, or standard pattern, ratios of the essential amino acids

Protein Quality for Ruminant Animals

  • Protein comes from two sources: microbial (obtained by microbial metabolism in the rumen) and food (not degradable in the rumen but is digestible in the abomasum)
  • RDP (rapidly degradable protein), SDP (slowly degradable protein), and ERDP (effective rumen degradable protein) are measures of protein quality for ruminant animals

This quiz covers the role of phosphorus in animal nutrition, its availability, and the importance of phytases in absorption. Relevant for Master Degree in Veterinary Medicine students.

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