Recombinant DNA Technology Overview

Recombinant DNA Technology

• Recombinant DNA is artificially created DNA from two or more sources, combined into a single molecule.
• This creates new genetic combinations useful for science, medicine, agriculture, and industry.
• Recombinant DNA technology is also known as genetic engineering.
• It emerged in 1968 with the discovery of restriction enzymes by Werner Arber.

Process of Recombinant DNA Technology

• DNA Extraction: Scientists extract DNA from the organism containing the gene of interest.

• Gene Cloning: Scientists isolate and create many copies of the desired gene.

• Gene Design: Genetic engineers modify the gene to function in a new organism. This is done in a test tube, cutting and rearranging gene sections.

• Transformation: The modified gene is inserted into the desired recipient cell using techniques like gene guns, agrobacterium, microfibers, or electroporation.

• Maintaining the recombinant DNA in the host and passing it to offspring is crucial.

• Inserting the desired gene into a host organism is challenging and requires careful selection of the vector and the host.

This quiz explores the fundamentals of Recombinant DNA Technology and its processes. Discover key concepts such as DNA extraction, gene cloning, and transformation techniques. Gain insights into the applications of genetic engineering in science and industry.