Recombinant DNA and Bacterial Vectors
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Questions and Answers

What is the function of restriction enzymes in producing rDNA?

Cut DNA in specific locations

What role does DNA ligase enzyme play in creating rDNA?

Pastes human DNA to the gap in the bacteria plasmid DNA

Explain the process of creating recombinant DNA using bacteria as vectors.

Donor DNA is fused with the vector's plasmid DNA, creating a new DNA combination added to the organism.

What is the significance of mass-producing rDNA in host cells?

<p>Useful only if it can be mass-produced as the host cell multiplies</p> Signup and view all the answers

Explain the concept of cloning in the context of producing identical copies of rDNA.

<p>Cloning simply means making identical copies of the rDNA.</p> Signup and view all the answers

What is the function of CRISPR (Cas9) in genetic engineering?

<p>CRISPR (Cas9) is used for precise gene editing.</p> Signup and view all the answers

What are the key differences in shape between DNA and RNA molecules?

<p>DNA has a double helix shape, while RNA is single-stranded.</p> Signup and view all the answers

Which bases are unique to DNA and not found in RNA?

<p>Thymine is unique to DNA and is not found in RNA.</p> Signup and view all the answers

What is the enzyme responsible for forming RNA through transcription?

<p>RNA polymerase is the enzyme responsible for forming RNA through transcription.</p> Signup and view all the answers

Where does DNA replication occur in a cell?

<p>DNA replication occurs in the nucleus of a cell.</p> Signup and view all the answers

Which enzyme is involved in DNA replication?

<p>DNA polymerase is the enzyme involved in DNA replication.</p> Signup and view all the answers

Why does DNA remain in the nucleus and not leave to perform protein synthesis?

<p>DNA remains in the nucleus to maintain its integrity and regulate the rate of protein synthesis.</p> Signup and view all the answers

What are the 3 main components of DNA?

<p>Phosphate group, deoxyribose sugar, nitrogen base</p> Signup and view all the answers

What are the two purine bases found in DNA?

<p>Adenine (A) and Guanine (G)</p> Signup and view all the answers

What is the function of Messenger RNA (mRNA) in protein synthesis?

<p>Carries DNA's message out of the nucleus to the ribosomes</p> Signup and view all the answers

What is the main purpose of DNA replication?

<p>To provide an exact copy of the DNA when cells divide</p> Signup and view all the answers

What is the main difference between DNA and RNA?

<p>DNA is double-stranded with thymine, while RNA is single-stranded with uracil</p> Signup and view all the answers

What is the significance of the 3 types of RNA (mRNA, rRNA, tRNA) in protein synthesis?

<p>They play roles in carrying the genetic message, forming ribosomes, and delivering amino acids</p> Signup and view all the answers

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