18 Questions
What is the function of restriction enzymes in producing rDNA?
Cut DNA in specific locations
What role does DNA ligase enzyme play in creating rDNA?
Pastes human DNA to the gap in the bacteria plasmid DNA
Explain the process of creating recombinant DNA using bacteria as vectors.
Donor DNA is fused with the vector's plasmid DNA, creating a new DNA combination added to the organism.
What is the significance of mass-producing rDNA in host cells?
Useful only if it can be mass-produced as the host cell multiplies
Explain the concept of cloning in the context of producing identical copies of rDNA.
Cloning simply means making identical copies of the rDNA.
What is the function of CRISPR (Cas9) in genetic engineering?
CRISPR (Cas9) is used for precise gene editing.
What are the key differences in shape between DNA and RNA molecules?
DNA has a double helix shape, while RNA is single-stranded.
Which bases are unique to DNA and not found in RNA?
Thymine is unique to DNA and is not found in RNA.
What is the enzyme responsible for forming RNA through transcription?
RNA polymerase is the enzyme responsible for forming RNA through transcription.
Where does DNA replication occur in a cell?
DNA replication occurs in the nucleus of a cell.
Which enzyme is involved in DNA replication?
DNA polymerase is the enzyme involved in DNA replication.
Why does DNA remain in the nucleus and not leave to perform protein synthesis?
DNA remains in the nucleus to maintain its integrity and regulate the rate of protein synthesis.
What are the 3 main components of DNA?
Phosphate group, deoxyribose sugar, nitrogen base
What are the two purine bases found in DNA?
Adenine (A) and Guanine (G)
What is the function of Messenger RNA (mRNA) in protein synthesis?
Carries DNA's message out of the nucleus to the ribosomes
What is the main purpose of DNA replication?
To provide an exact copy of the DNA when cells divide
What is the main difference between DNA and RNA?
DNA is double-stranded with thymine, while RNA is single-stranded with uracil
What is the significance of the 3 types of RNA (mRNA, rRNA, tRNA) in protein synthesis?
They play roles in carrying the genetic message, forming ribosomes, and delivering amino acids
Test your knowledge on the use of bacteria as vectors for creating recombinant DNA. Learn about the process of inserting donor DNA into bacterial plasmids using restriction enzymes and DNA ligase. Understand the importance of mass-producing recombinant DNA in host cells.
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