rDNA Technology: Steps and Enzymes

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What is the purpose of using ice-minus Pseudomonas syringae in genetic engineering?

To produce a protein that forms a nucleus for the formation of ice crystals

To enhance the growth rate of plants

To increase the resistance of plants to frost damage (correct)

To produce antibiotics

What is the primary goal of genetic fusion in genetic engineering?

To delete a DNA segment

To transfer genes from one location to another on a chromosome (correct)

To produce antibiotics

To amplify genes

Which of the following is an example of gene amplification?

Rapid reproduction of bacteriophages to increase antibiotic yields (correct)

Removing cell walls of two strains and mixing protoplasts

Coupling of two operons

Transposition of genes from one location to another on a chromosome

What is the result of protoplast fusion in genetic engineering?

New strains that have desirable characters Signup and view all the answers

Which of the following is NOT a method of genetic engineering?

Cell wall synthesis Signup and view all the answers

What is the primary purpose of restriction endonucleases in recombinant DNA technology?

To cut DNA at specific locations Signup and view all the answers

Which of the following organisms is used in genetic engineering to increase the resistance of plants to frost damage?

Pseudomonas syringae Signup and view all the answers

What is the characteristic of Type II restriction endonucleases that makes them ideal for gene cloning?

They cut DNA in a predictable and consistent manner Signup and view all the answers

What is the purpose of the ligase enzyme in recombinant DNA technology?

To join DNA fragments Signup and view all the answers

What is the typical length of recognition sequences for Type II restriction endonucleases?

4 to 8 nucleotides Signup and view all the answers

What is the name of the restriction endonuclease that was first characterized in Escherichia coli?

EcoRI Signup and view all the answers

Why are Type I and Type III restriction endonucleases of little practical use in gene cloning?

They cut DNA unpredictably and require ATP Signup and view all the answers

What is the primary purpose of recombinant DNA technology?

To obtain enhanced and desired characteristics in living organisms or their products Signup and view all the answers

What is involved in recombinant DNA technology?

Inserting DNA fragments from various sources into a vector Signup and view all the answers

What is the result of combining DNA from different species using recombinant DNA technology?

Genes with new functions Signup and view all the answers

What is the purpose of genetic engineering in Xanthomonas campestris?

To utilize lactose from whey for xanthan production Signup and view all the answers

What is the problem associated with whey in the dairy industry?

Disposal of large quantities of whey is a major problem Signup and view all the answers

What is the role of enzymes in recombinant DNA technology?

To manipulate and isolate DNA segments of interest Signup and view all the answers