30 Questions
What is the purpose of PCR in rDNA technology?
To amplify specific DNA sequences
What is the role of primers in PCR?
To mark the beginning and end of the DNA fragment to be amplified
What is the advantage of using PCR in DNA analysis?
It allows researchers to generate sufficient quantities of DNA for analysis even from limited starting material
What is the outcome of PCR?
Millions to billions of copies of a specific segment of DNA
What is the process of obtaining the gene of interest apart from PCR?
Gene synthesis
What is the common method of cloning used in vitro?
PCR
What is recombinant DNA technology used for?
To manipulate and alter DNA sequences
Who developed the recombinant DNA technology in 1973?
Boyer and Cohen
What is one of the applications of recombinant DNA technology in medicine?
Gene therapy
What is the term for the direct manipulation of an organism's genes?
All of the above
What is an example of a therapeutic protein produced using recombinant DNA technology?
Insulin
What is the primary function of ZOLGENSMA?
To deliver a functional copy of the SMN1 gene to motor neurons
What is the name of the treatment developed by Novartis for spinal muscular atrophy (SMA) using recombinant DNA technology?
Zolgensma
What is the purpose of inserting genes encoding antigens from pathogens into vectors?
To stimulate an immune response without causing disease
What is the first step in recombinant DNA production?
Generation of the gene of interest
What is the role of a vector in recombinant DNA technology?
To insert the gene of interest
What is the result of using the gene presenting the spike protein found on the surface of the SARS-CoV-2 virus?
Stimulation of an immune response against COVID-19
What is the purpose of gene selection and expression in recombinant DNA production?
To express the gene and produce the desired protein
What is the primary function of a plasmid vector?
To introduce a gene of interest into a host cell
What is the purpose of a selection marker in a plasmid?
To identify and eliminate non-transformants
What is the function of the origin of replication (Ori) in a plasmid?
To enable the plasmid to replicate independently of the host genome
What is the purpose of PCR amplification in phylogenetic analysis?
To identify and classify organisms
What is the purpose of a multiple cloning site (MCS) in a plasmid?
To provide multiple unique restriction enzyme recognition sites
What type of vector carries a dual function, introducing a gene of interest and expressing the DNA insert for producing a specific protein?
Expression vector
What is molecular cloning?
A collection of methods to create copies of DNA fragments
What is a characteristic feature of plasmids?
They are physically separate from chromosomal DNA
What is the purpose of a vector in molecular cloning?
To transfer foreign DNA fragments into a new cell
What is site-directed mutagenesis used for?
To study the effects of mutations on gene function
What is DNA amplification used for in archeological remains?
To improve the quality and quantity of DNA samples
What is cloning?
The process of creating an identical copy of an organism
This quiz covers the basics of Recombinant DNA (rDNA) technology, a technique used to manipulate and alter DNA sequences by combining DNA molecules from different sources. It was developed by Boyer and Cohen in 1973.
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