4th Level Biotechnology Lecture 5: Recombinant DNA (rDNA) Technology

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What is the purpose of PCR in rDNA technology?

To sequence DNA

To amplify specific DNA sequences (correct)

To express genes

To synthesize genes

What is the role of primers in PCR?

To mark the beginning and end of the DNA fragment to be amplified (correct)

To amplify DNA sequences

To clone genes

To synthesize DNA

What is the advantage of using PCR in DNA analysis?

It allows researchers to generate sufficient quantities of DNA for analysis even from limited starting material (correct)

It reduces the quantity of DNA required

It generates insufficient quantities of DNA for analysis

It is a time-consuming process

What is the outcome of PCR?

Millions to billions of copies of a specific segment of DNA Signup and view all the answers

What is the process of obtaining the gene of interest apart from PCR?

Gene synthesis Signup and view all the answers

What is the common method of cloning used in vitro?

PCR Signup and view all the answers

What is recombinant DNA technology used for?

To manipulate and alter DNA sequences Signup and view all the answers

Who developed the recombinant DNA technology in 1973?

Boyer and Cohen Signup and view all the answers

What is one of the applications of recombinant DNA technology in medicine?

Gene therapy Signup and view all the answers

What is the term for the direct manipulation of an organism's genes?

All of the above Signup and view all the answers

What is an example of a therapeutic protein produced using recombinant DNA technology?

Insulin Signup and view all the answers

What is the primary function of ZOLGENSMA?

To deliver a functional copy of the SMN1 gene to motor neurons Signup and view all the answers

What is the name of the treatment developed by Novartis for spinal muscular atrophy (SMA) using recombinant DNA technology?

Zolgensma Signup and view all the answers

What is the purpose of inserting genes encoding antigens from pathogens into vectors?

To stimulate an immune response without causing disease Signup and view all the answers

What is the first step in recombinant DNA production?

Generation of the gene of interest Signup and view all the answers

What is the role of a vector in recombinant DNA technology?

To insert the gene of interest Signup and view all the answers

What is the result of using the gene presenting the spike protein found on the surface of the SARS-CoV-2 virus?

Stimulation of an immune response against COVID-19 Signup and view all the answers

What is the purpose of gene selection and expression in recombinant DNA production?

To express the gene and produce the desired protein Signup and view all the answers

What is the primary function of a plasmid vector?

To introduce a gene of interest into a host cell Signup and view all the answers

What is the purpose of a selection marker in a plasmid?

To identify and eliminate non-transformants Signup and view all the answers

What is the function of the origin of replication (Ori) in a plasmid?

To enable the plasmid to replicate independently of the host genome Signup and view all the answers

What is the purpose of PCR amplification in phylogenetic analysis?

To identify and classify organisms Signup and view all the answers

What is the purpose of a multiple cloning site (MCS) in a plasmid?

To provide multiple unique restriction enzyme recognition sites Signup and view all the answers

What type of vector carries a dual function, introducing a gene of interest and expressing the DNA insert for producing a specific protein?

Expression vector Signup and view all the answers

What is molecular cloning?

A collection of methods to create copies of DNA fragments Signup and view all the answers

What is a characteristic feature of plasmids?

They are physically separate from chromosomal DNA Signup and view all the answers

What is the purpose of a vector in molecular cloning?

To transfer foreign DNA fragments into a new cell Signup and view all the answers

What is site-directed mutagenesis used for?

To study the effects of mutations on gene function Signup and view all the answers

What is DNA amplification used for in archeological remains?

To improve the quality and quantity of DNA samples Signup and view all the answers

What is cloning?

The process of creating an identical copy of an organism Signup and view all the answers