25 Questions
What is the color of the diketohydrin complex formed at the end of the reaction?
Deep purple
Which of the following amino acids yields an iminium salt in this test?
Proline and Hydroxyproline
What is the color of the iminium salt formed in proline and hydroxyproline?
Yellow-orange
What solution is taken in one test tube?
Standard solution
What is the concentration of the standard protein solution?
1% protein solution
What is formed at the end of the reaction?
Diketohydrin complex
Which of the following statements is true about the reaction?
It forms a deep purple complex
Which of the following is NOT a name for the standard solution?
Sample solution
In which tube is the sample solution taken?
Another test tube
What is the composition of the sample solution?
Amino acid solutions
What is the primary function of a water bath in the protein test?
To provide a gentle and consistent heat source
What is observed during the protein test?
Formation of color
What is the purpose of a beaker with heated water in the protein test?
To provide a gentle and consistent heat source
What is the goal of the protein test?
To identify the type of protein
What is the role of heat in the protein test?
To provide a gentle and consistent heat source
What is the purpose of a test tube stand in the experiment?
To support the test tubes
What is added to the test tubes in step 2 of the experiment?
A few drops of ninhydrin reagent
What is the primary function of the test tubes in the experiment?
To hold the reaction mixture
What is not a material required for the experiment?
Gloves
How many test tubes are used in the experiment?
2
What happens to the color of the nitro derivative salts when alkali is added?
It changes from yellow to orange
What is the purpose of adding alkali to the nitro derivative salts?
To change the color of the solution
What type of salts are involved in the reaction described?
Nitro derivative salts
What is observed when alkali is added to the nitro derivative salts?
A change in color
What is the role of alkali in the reaction described?
It induces a color change
Study Notes
Ninhydrin Test for Proteins
- The reaction forms a diketohydrin complex, which has a deep purple color.
Amino Acids like Proline and Hydroxyproline
- In these amino acids, the test yields an iminium salt, which is yellow-orange in color.
Materials Required
- 1 mL of standard protein solution (1% protein solution)
- 1 mL of the test sample (amino acids solutions)
- Test tubes
- Test tube stand
- Water bath (or beaker with heated water)
- Ninhydrin reagent
Procedure
- Add a few drops of ninhydrin reagent to both test tubes.
- Observe the formation of color and note down the result.
Color Change
- On adding alkali to these nitro derivative salts, the color changes from yellow to orange.
This quiz assesses understanding of the reaction test for proline and hydroxyproline, resulting in the formation of a diketohydrin complex and a yellow-orange iminium salt.
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