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Questions and Answers
Which of the following is NOT a major class of lipids?
Which of the following is NOT a major class of lipids?
What is a significant feature of essential fatty acids?
What is a significant feature of essential fatty acids?
Which of the following fatty acids contains three double bonds?
Which of the following fatty acids contains three double bonds?
What role do waxes play in plants?
What role do waxes play in plants?
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Which class of secondary metabolites are known for their role in plant color and attracting pollinators?
Which class of secondary metabolites are known for their role in plant color and attracting pollinators?
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Which sugars are commonly found in glycosides produced by plants?
Which sugars are commonly found in glycosides produced by plants?
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Which of the following secondary metabolites is selectively produced by specific plants?
Which of the following secondary metabolites is selectively produced by specific plants?
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What are epicuticular waxes known for?
What are epicuticular waxes known for?
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Which of the following is classified as a primary metabolite in plants?
Which of the following is classified as a primary metabolite in plants?
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What role do primary metabolites play in plants?
What role do primary metabolites play in plants?
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Which of the following statements about primary metabolites is true?
Which of the following statements about primary metabolites is true?
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Carbohydrates are considered primary metabolites because they fulfill which of the following functions?
Carbohydrates are considered primary metabolites because they fulfill which of the following functions?
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Which type of carbohydrate is a polymer made up of many sugar units?
Which type of carbohydrate is a polymer made up of many sugar units?
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What is the primary role of structural polysaccharides like cellulose in plants?
What is the primary role of structural polysaccharides like cellulose in plants?
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Which of the following is NOT a primary metabolite?
Which of the following is NOT a primary metabolite?
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What distinguishes secondary metabolites from primary metabolites in plants?
What distinguishes secondary metabolites from primary metabolites in plants?
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What characteristic distinguishes primary metabolites from secondary metabolites?
What characteristic distinguishes primary metabolites from secondary metabolites?
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Which of the following is an example of a secondary metabolite?
Which of the following is an example of a secondary metabolite?
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During which phase of cell growth are primary metabolites produced?
During which phase of cell growth are primary metabolites produced?
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How do secondary metabolites contribute to plant survival?
How do secondary metabolites contribute to plant survival?
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Which of the following statements is true regarding the production of primary metabolites?
Which of the following statements is true regarding the production of primary metabolites?
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What is a notable feature of secondary metabolites in plants?
What is a notable feature of secondary metabolites in plants?
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Which biotechnology strategy directly involves the regeneration of plants from plant cells?
Which biotechnology strategy directly involves the regeneration of plants from plant cells?
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How do primary metabolites impact the metabolic pathways of plants?
How do primary metabolites impact the metabolic pathways of plants?
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Study Notes
Biotechnology for Plantation Crops - Chapter 6
- Metabolism & Metabolites: Metabolism encompasses the chemical processes within a living organism to sustain life. Metabolites are the intermediates and products of this metabolism. They are typically small molecules/compounds with diverse functions like structure, signaling, stimulation, and defense.
Metabolic Engineering in Plants
- Plant Factories for Small Molecules: Plants are highly prolific producers of small molecules.
- Metabolite Count (2004): Over 100,000 metabolites were identified in 2004, but this is estimated to be only 10% of the total.
- Secondary Metabolites in Plants: Among various organisms, plants possess the largest quantities of secondary metabolites, ranging from 5,000 to 25,000 per plant.
Plant Metabolism
- Photosynthesis, Respiration, and Photorespiration: A diagram illustrates these processes, highlighting the inputs (CO2, H2O, light energy) and outputs (sugars, O2, H20) of each within the plant.
Plant Metabolism Outline
- Photosynthesis: This process uses carbon dioxide, water, and light energy to produce sugars, which are stored as starch in storage organs.
- Respiration: The process uses sugars and oxygen to release energy. Also shown in the diagram that respiration doesn't involve photorespiration.
- Outputs/Products: Sugars, Starch (stored food), Pectin, Cellulose (cell walls), Lignin, Cutin, Fats, Membranes, Proteins (enzymes), Pigments, Hormones, Vitamins, Alkaloids, tannins, and other protective substances.
- Inputs/Requirements: Water, Carbon Dioxide, Oxygen, Soil Minerals, light energy, and energy produced by respiration.
Primary Metabolites
- Role in Plant Growth: Primary metabolites are compounds involved in the normal growth, development, and reproduction of all plants.
- Main Types: Carbohydrates, proteins, nucleic acids, and lipids.
- Precursors for Secondary Production: Some primary metabolites act as precursors for the production of secondary metabolites.
Secondary Metabolites
- Not Directly Involved in Growth: Secondary metabolites are not involved in the plant's direct growth, development, and reproduction.
- Ecological Functions (Important): They often have important ecological functions like defense, attracting pollinators, and seed dispersal.
- Examples: Lignin, anthocyanin, phytoalexin, alkaloids, are examples.
Carbohydrates
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Types of Carbohydrates:
- Monosaccharides: Single sugar units, such as glucose and fructose.
- Disaccharides: Two sugar units combined, like sucrose (glucose + fructose).
- Polysaccharides: Multiple sugar units linked together forming polymers.
Polysaccharides
- Structural Support: Structural polysaccharides such as cellulose provide structural support to plants.
- Energy Storage: Storage polysaccharides, like starch, are used to store energy for later use by the plant.
Structural Polysaccharides ( Cellulose, etc)
- Cellulose: A major structural component in plants, contributing significantly (40-60%) to cell walls. It is also a very common polymer in the environment.
- Chemical Strength: Its strength stems from its particular chemical structure, involving long beta-glucose chains.
- Beta-glucose Chains: These long beta-glucose molecules (100 to 15,000) are linked together
Proteins
- Plant Biomass: Proteins make up a substantial portion of the remaining biomass in plants.
- Amino Acid Building Blocks: Proteins are chains (one or more polypeptides) of amino acids.
- Photosynthesis for Amino Acid Production: Plants synthesize amino acids from the products of photosynthesis through complex processes involving nitrogen acquisition (often in the form of NH3). Energy (ATP and NADPH) is required.
- Structural Proteins: Structural proteins comprise 2-10% of cell walls. Expansins, extensins, act to increase surface area and help protect or repair cell walls. Plant cell membranes are composed of about 50 % structural proteins.
- Storage Proteins: These proteins are primarily stored in seeds and provide nutrition to seedlings during early development. Different plant species produce different storage proteins (ex: Zein in corn, Gliadin in wheat).
Enzymes
- Biochemical Catalysts: Enzymes catalyze biochemical reactions in living cells.
- Commercial Importance: Some enzymes maintain activity when separated from plants making them commercially important. (ex: Papain and Bromelain)
Nucleic Acids
- Complex Biological Polymers: Nucleic acids (RNA and DNA) are the most complex biological polymers in organisms.
- Basic Components: Nucleic acids are composed of basic components—adenine, thymine, guanine, and cytosine—that share similar structures in all plants.
Lipids
- Non-polar Solvent Solubility: Lipids are substances that dissolve in non-polar solvents like ether, chloroform, and acetone but not in water.
- Energy Storage: Some lipids store energy.
- Membrane Components: Some lipids are the primary components of biological membranes.
- Other Functions: Some lipids also function as hormones, antioxidants, and pigments.
Lipids (continued)
- Hydrocarbon Groups: Most lipids are made of non-polar hydrocarbon groups like CH3, CH2, and CH.
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Major Lipids:
- Fatty Acids
- Triacylglycerols
- Waxes
- Phospholipids
Oils
- Plant Component: Oils occur in all plant parts, but are typically most abundant in seeds.
- Commercial Harvesting: Some seeds have sufficient oil content for commercial harvesting, like palm oil, cottonseed oil, sesame oil etc.
- Common Fatty Acid Components: Common fatty acids in seed oils include oleic (one double bond), linoleic (two double bonds), and linolenic (three double bonds) acid .
- Essential Fatty Acids: Linoleic and linolenic acid are considered essential fatty acids.
Waxes
- Complex Mixtures: Waxes are complex mixtures of fatty acids and long-chain alcohols.
- Plant Protection: Waxes form the outer layer of leaves, fruits, and herbaceous stems in plants, protecting them from water loss. These waxes are often called epicuticular waxes and cutin.
- Different Types in Plants: Plant's also have cuticular waxes, suberin ( a wax found in bark and plant roots) which also help with water preservation.
Plant Secondary Metabolites (Part 2)
- Variety of Compounds: Plants produce diverse compounds (e.g., morphine, caffeine, nicotine, menthol, rubber) that are not as widely distributed as primary metabolites.
- Ecological Interactions: Certain secondary metabolites play crucial roles in plant-to-plant, and plant-to-organism interactions and ecology.
Plant Secondary Metabolites (continued)
- Classification Based on Structure and Pathways: Secondary compounds are categorized based on similar structures, biosynthetic pathways, or the specific types of plants.
- Common Classes: The major classes of secondary metabolites often include alkaloids, terpenoids, and phenolics.
- Glycosides as Compounds: Many secondary metabolites are often found in combination with sugars, forming compounds called glycosides. The sugars are typically glucose, galactose, or rhamnose; however, some plant species have unique sugars.
Functions of Secondary Metabolites
- Pigments: Many secondary compounds are colorful pigments (e.g., anthocyanins), aiding in attracting pollinators and dispersing seeds.
- Protection Against Herbivores/Microbes: Secondary metabolites like nicotine and other toxic compounds help safeguard plants from herbivores and microbes.
- Support and Transport in Plants: Lignin is a complex polymer that provides support to vascular tissue allowing water and nutrients to move throughout the plant.
Alkaloids
- Natural Compounds (with Nitrogen): Alkaloids are naturally occurring chemical compounds that contain mostly basic nitrogen atoms.
- Varied Toxicity: Many alkaloids are toxic to other organisms, while some are medicinally used (e.g., morphine) or as recreation drugs. They often have pharmacological effects.
- Large Class: Over 6500 types of alkaloids are known.
Terpenoids
- Isoprene-based: Terpenoids are dimers and polymers of isoprene (C5H8).
- Plant Flavors and Smells: They contribute considerable aroma, scents (eucalyptus), flavors (cinnamon, cloves, ginger) and colors (yellow in sunflowers, red in tomatoes) in plants.
- Important Examples: Citral, menthol, camphor are examples of well-known terpenoids.
Phenolics
- Hydroxyl-aromatic: These chemical compounds have a hydroxyl group (-OH) directly attached to an aromatic hydrocarbon ring or benzene ring.
- Flavor/Other Plant Functions: Various phenolics contribute to flavors (nutmeg) colors, and often have astringent tastes.
- Important Examples: Myristicin, Flavonoids (rutin), Anthocyanins, and Tannins are illustrative examples.
- Lignin (structural): A major constituent of wood and is a complex polymer that supports the plant structures, playing a role in structural support and transport of fluids, allowing water/nutrients to pass throughout.
Enhancing Product Yield in Major Crops Through Biotechnology
- Breeding Strategies: Techniques to select and propagate plants with high yield traits, ranging from basic techniques to sophisticated molecular approaches like markers (DNA fingerprinting).
- Plant Cloning via Tissue Culture: Producing copies of plants through cloning techniques to quickly create numerous copies of crops with high yields. This can also decrease the spread of pathogens and disease in plants.
- Genetic Engineering/Modification: Introducing new traits (e.g., higher yields) into plants through genetic engineering; for example, engineering oil palm crops to enhance their yield.
- Biofertilizers: Enhancing nutrient availability to the plant. Biological fertilizers; such as those using Mycorrhiza use symbiosis to provide larger root surface area to absorb nutrients more efficiently.
- Biocontrol: Using biological methods to control pests and diseases (ex: Bt, Metarhizium, Trichoderma, Ganoderma).
Primary vs Secondary Metabolites
- Primary Metabolites (Essential): Essential for growth, implicated directly in metabolic processes like respiration and photosynthesis. They are accumulated in substantial quantities. Primary metabolites lack involvement in defense activities.
- Secondary Metabolites (Additional): Involved in defense reactions. They are accumulated in smaller quantities. They have roles that go beyond basic plant function such as contributing colors and flavors and are not necessary for basic survival, however, play important roles in plant-plant and plant-organism interactions.
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Test your knowledge about lipids and secondary metabolites in plants. This quiz covers essential fatty acids, glycosides, and the roles of various plant compounds. Challenge yourself to see how well you understand the features and classifications of these important biological molecules.