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Questions and Answers
What was the purpose of digesting the nucleic acids with RNAase A?
What was the purpose of digesting the nucleic acids with RNAase A?
- To separate DNA from RNA fragments
- To remove RNA from the DNA preparation (correct)
- To ensure the DNA was in a supercoiled form
- To degrade any remaining RNA in the sample
What was the purpose of passing the digested nucleic acids through the Bio-Gel A50m column?
What was the purpose of passing the digested nucleic acids through the Bio-Gel A50m column?
- To further purify the DNA from the RNA fragments (correct)
- To concentrate the DNA for downstream applications
- To remove any residual RNAase A from the sample
- To separate the plasmids based on their size
What was the composition of the DNA preparation used in these experiments?
What was the composition of the DNA preparation used in these experiments?
- Mostly supercoiled plasmids with a small amount of nicked circles and linear fragments
- 1/3 supercoiled plasmids, 1/3 nicked circles, and 1/3 larger oligomers of the plasmid (correct)
- 1/2 supercoiled plasmids and 1/2 nicked circles
- 1/3 supercoiled plasmids, 1/3 nicked circles, and 1/3 linear DNA fragments
What was the purpose of using cytochalasin B during the injection of the DNA into the pronucleus?
What was the purpose of using cytochalasin B during the injection of the DNA into the pronucleus?
What was the volume of DNA solution injected into the male pronucleus?
What was the volume of DNA solution injected into the male pronucleus?
What was the purpose of washing the ova free of cytochalasin B after the injection?
What was the purpose of washing the ova free of cytochalasin B after the injection?
What was the purpose of the thymidine kinase assay described in the text?
What was the purpose of the thymidine kinase assay described in the text?
What was the main purpose of the nucleic acid isolation procedure described in the text?
What was the main purpose of the nucleic acid isolation procedure described in the text?
What was the purpose of the $eta$-mercaptoethanol included in the thymidine kinase assay reaction mixture?
What was the purpose of the $eta$-mercaptoethanol included in the thymidine kinase assay reaction mixture?
What was the purpose of the proteinase K used during the nucleic acid isolation procedure?
What was the purpose of the proteinase K used during the nucleic acid isolation procedure?
What was the purpose of the DE-81 paper used in the thymidine kinase assay?
What was the purpose of the DE-81 paper used in the thymidine kinase assay?
What was the purpose of the NaF included in the thymidine kinase assay reaction mixture?
What was the purpose of the NaF included in the thymidine kinase assay reaction mixture?
What was used to label DNA probes in the described experiment?
What was used to label DNA probes in the described experiment?
Which enzyme was NOT mentioned in the text for restriction digestion?
Which enzyme was NOT mentioned in the text for restriction digestion?
What was the purpose of using RNAase A in the restriction digests?
What was the purpose of using RNAase A in the restriction digests?
What specific conditions were used for the hybridizations in the described experiment?
What specific conditions were used for the hybridizations in the described experiment?
Which fragment was used to prepare the 32P-cDNA?
Which fragment was used to prepare the 32P-cDNA?
What method was used to transfer DNA onto nitrocellulose in the experiment?
What method was used to transfer DNA onto nitrocellulose in the experiment?
