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Mastering Cell Lysis and Protein Denaturation
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Mastering Cell Lysis and Protein Denaturation

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Questions and Answers

Which method is used for denaturation and precipitation of proteins from nucleic acid solution?

  • Nonionic detergent
  • Organic solvents (correct)
  • RNAse treatment
  • Protease treatment

    • What is the purpose of RNAse treatment in this method?

  • To denature proteins
  • To remove cell debris
  • To concentrate DNA
  • To remove unwanted RNA (correct)

    • Which step follows cell lysis in this method?

  • RNAse treatment
  • Precipitation with ethanol
  • Centrifugation (correct)
  • Protease treatment

    • What is the purpose of precipitation with ice-cold ethanol in this method?

    <p>To concentrate DNA</p> Signup and view all the answers

    What can be used to recover the nucleic acid precipitate in this method?

    <p>Centrifugation</p> Signup and view all the answers

    Which of the following is used for cell lysis in this method?

    <p>Sodium dodecyl sulfate</p> Signup and view all the answers

    What is the purpose of protease treatment in this method?

    <p>To denature proteins</p> Signup and view all the answers

    Which of the following is NOT used for denaturation and precipitation of proteins from nucleic acid solution?

    <p>Sodium dodecyl sulfate</p> Signup and view all the answers

    What is the purpose of RNAse treatment in this method?

    <p>To remove unwanted RNA</p> Signup and view all the answers

    What is used to recover the nucleic acid precipitate in this method?

    <p>Ethanol</p> Signup and view all the answers

    Which step in the method involves annealing short primers to the single-stranded DNA template?

    <p>Denaturation</p> Signup and view all the answers

    What determines the temperature for the annealing step in this method?

    <p>The melting temperature of the primer</p> Signup and view all the answers

    What is the purpose of annealing in this method?

    <p>To denature the DNA template</p> Signup and view all the answers

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