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Questions and Answers
Which method is used for denaturation and precipitation of proteins from nucleic acid solution?
Which method is used for denaturation and precipitation of proteins from nucleic acid solution?
- Nonionic detergent
- Organic solvents (correct)
- RNAse treatment
- Protease treatment
What is the purpose of RNAse treatment in this method?
What is the purpose of RNAse treatment in this method?
- To denature proteins
- To remove cell debris
- To concentrate DNA
- To remove unwanted RNA (correct)
Which step follows cell lysis in this method?
Which step follows cell lysis in this method?
- RNAse treatment
- Precipitation with ethanol
- Centrifugation (correct)
- Protease treatment
What is the purpose of precipitation with ice-cold ethanol in this method?
What is the purpose of precipitation with ice-cold ethanol in this method?
What can be used to recover the nucleic acid precipitate in this method?
What can be used to recover the nucleic acid precipitate in this method?
Which of the following is used for cell lysis in this method?
Which of the following is used for cell lysis in this method?
What is the purpose of protease treatment in this method?
What is the purpose of protease treatment in this method?
Which of the following is NOT used for denaturation and precipitation of proteins from nucleic acid solution?
Which of the following is NOT used for denaturation and precipitation of proteins from nucleic acid solution?
What is the purpose of RNAse treatment in this method?
What is the purpose of RNAse treatment in this method?
What is used to recover the nucleic acid precipitate in this method?
What is used to recover the nucleic acid precipitate in this method?
Which step in the method involves annealing short primers to the single-stranded DNA template?
Which step in the method involves annealing short primers to the single-stranded DNA template?
What determines the temperature for the annealing step in this method?
What determines the temperature for the annealing step in this method?
What is the purpose of annealing in this method?
What is the purpose of annealing in this method?
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