Podcast
Questions and Answers
What is the main purpose of gel electrophoresis?
What is the main purpose of gel electrophoresis?
- To visualize RNA strands
- To separate DNA fragments by size (correct)
- To replicate DNA segments in host cells
- To link foreign DNA to plasmids
Which of the following is commonly used as a medium for gel electrophoresis?
Which of the following is commonly used as a medium for gel electrophoresis?
- Agarose (correct)
- Silica gel
- Sodium alginate
- Polyacrylamide
What is a characteristic of plasmids as cloning vectors?
What is a characteristic of plasmids as cloning vectors?
- They can have one to a hundred or more copies per cell (correct)
- They cannot replicate within bacterial cells
- They always contain multiple insert sites
- They have a low copy number in host cells
How do DNA fragments appear after being visualized using ethidium bromide?
How do DNA fragments appear after being visualized using ethidium bromide?
Why is it important to cut the vector and source DNA with the same restriction enzyme?
Why is it important to cut the vector and source DNA with the same restriction enzyme?
Flashcards
Gel electrophoresis
Gel electrophoresis
A technique used to separate DNA fragments based on size.
Cloning vector
Cloning vector
A DNA molecule used to carry foreign DNA into a host cell.
Agarose gel
Agarose gel
A gel matrix used in gel electrophoresis to separate DNA fragments.
DNA fragment separation
DNA fragment separation
Signup and view all the flashcards
Recombinant vector
Recombinant vector
Signup and view all the flashcards
